This chapter investigates integrating agricultural biosecurity measures with environmental sustainability practices to bolster food safety in Saudi Arabia. It emphasizes the synergy between cutting-edge technologies, effective stakeholder engagement, and adaptive strategies to climate change, underscoring their pivotal roles in developing resilient agricultural systems. Through a comparative analysis with global standards, the chapter elucidates Saudi Arabia’s position and advancements in agricultural biosecurity, presenting an array of innovative technologies—from precision agriculture tools to AI-driven pest detection systems—that exemplify the country’s commitment to sustainable food production. Specific initiatives, such as the National Center for Prevention and Control of Plant Pests and Animal Diseases (NCPCPPAD), are highlighted to illustrate practical applications. The chapter offers a multifaceted view of the challenges and successes of implementing biosecurity measures by incorporating comprehensive stakeholder perspectives, including those from farmers, policymakers, and scientists. Furthermore, it explores the impacts of climate change on agricultural biosecurity and delineates Saudi Arabia’s proactive approaches to mitigating these effects. Key findings include significant improvements in water consumption efficiency and crop yields. By weaving together interdisciplinary research, case studies, and policy analysis, the chapter seeks to contribute to the global discourse on securing food systems against biosecurity threats while advancing environmental sustainability. Future directions for research and policy are also discussed.