Rewiring photosynthesis: a photosystem I-hydrogenase chimera that makes H<sub>2</sub><i>in vivo</i>

Kanygin, Andrey; Milrad, Yuval; Chandrasekhar, Thummala; Reifschneider, Kiera; Baker, Patricia L.; Marco, Pini; Yacoby, Iftach; Redding, Kevin

doi:10.1039/c9ee03859k

Cited by 62 publications

(74 citation statements)

References 54 publications

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“…Genetic fusions could be employed for the coupling of sink acceptors to PSI. A number of analogous fusions have been reported to increase electron transfer in the literature, including cytochrome P450 fusion to PsaM (54) or Fd (53,55), or hydrogenase fusion to PsaC (56), PsaD (57), PsaE (58), or Fd (59). Reducing the affinity of FNR (Fd-NADP + -oxidoreductase) interaction by point mutations could be used as strategy to redirect photosynthetic electrons through heterologous production pathways (60).…”

Section: Discussionmentioning

confidence: 99%

Improved photosynthetic capacity and photosystem I oxidation via heterologous metabolism engineering in cyanobacteria

Santos-Merino

Torrado

Davis

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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Cyanobacteria must prevent imbalances between absorbed light energy (source) and the metabolic capacity (sink) to utilize it to protect their photosynthetic apparatus against damage. A number of photoprotective mechanisms assist in dissipating excess absorbed energy, including respiratory terminal oxidases and flavodiiron proteins, but inherently reduce photosynthetic efficiency. Recently, it has been hypothesized that some engineered metabolic pathways may improve photosynthetic performance by correcting source/sink imbalances. In the context of this subject, we explored the interconnectivity between endogenous electron valves, and the activation of one or more heterologous metabolic sinks. We coexpressed two heterologous metabolic pathways that have been previously shown to positively impact photosynthetic activity in cyanobacteria, a sucrose production pathway (consuming ATP and reductant) and a reductant-only consuming cytochrome P450. Sucrose export was associated with improved quantum yield of phtotosystem II (PSII) and enhanced electron transport chain flux, especially at lower illumination levels, while cytochrome P450 activity led to photosynthetic enhancements primarily observed under high light. Moreover, coexpression of these two heterologous sinks showed additive impacts on photosynthesis, indicating that neither sink alone was capable of utilizing the full “overcapacity” of the electron transport chain. We find that heterologous sinks may partially compensate for the loss of photosystem I (PSI) oxidizing mechanisms even under rapid illumination changes, although this compensation is incomplete. Our results provide support for the theory that heterologous metabolism can act as a photosynthetic sink and exhibit some overlapping functionality with photoprotective mechanisms, while potentially conserving energy within useful metabolic products that might otherwise be “lost.”

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Section: Discussionmentioning

confidence: 99%

Improved photosynthetic capacity and photosystem I oxidation via heterologous metabolism engineering in cyanobacteria

Santos-Merino

Torrado

Davis

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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“…7 Recently both the [NiFe] and [FeFe] hydrogenases have been fused with photosystem I for solar H 2 production, with protons and electrons provided by photosystem II water splitting. 8,9 This perspective focuses on the biosynthesis of the [FeFe] hydrogenase, whose catalytic core is the "H-cluster" which consists of a standard [4Fe-4S] subcluster, denoted as [4Fe] H , linked to a unique binuclear [2Fe] H subcluster via a bridging cysteine residue (Fig. 1).…”

Section: Introduction To [Fefe] Hydrogenasementioning

confidence: 99%

Biosynthesis of the catalytic H-cluster of [FeFe] hydrogenase: the roles of the Fe–S maturase proteins HydE, HydF, and HydG

2020

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“…This synthetic construct promoted the self-assembly of the PSI and hydrogenase portions to yield an active in vivo ensemble. Interestingly the algal cells expressing this PSI-hydrogenase chimera continued to make H2 in a light-dependent fashion for several days [94].…”

Section: Overcoming Kinetic Limitations Of Hydrogen Evolutionmentioning

confidence: 99%

Green Catalysts: Applied and Synthetic Photosynthesis

Teodor¹,

Sherman²,

Ison³

et al. 2020

Preprint

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The biological process of photosynthesis was critical in catalyzing the oxygenation of Earth’s atmosphere 2.5 billion years ago, changing the course of development of life on Earth. Recently, the fields of applied and synthetic photosynthesis have utilized the light-driven protein-pigment supercomplexes central to photosynthesis for the photocatalytic production of fuel and other various valuable products. The reaction center Photosystem I is of particular interest in applied photosynthesis due to its high stability post-purification, non-geopolitical limitation, and its ability to generate the greatest reducing power found in Nature. These remarkable properties have been harnessed for the photocatalytic production of a number of valuable products in the applied photosynthesis research field. These primarily include photocurrents and molecular hydrogen as fuels. The use of artificial reaction centers to generate substrates and reducing equivalents to drive non-photoactive enzymes for valuable product generation has been a long-standing area of interest of the synthetic photosynthesis research field. In this review, we cover advances in these areas and further speculate synthetic and applied photosynthesis as photocatalysts for the generation of valuable products.

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Rewiring photosynthesis: a photosystem I-hydrogenase chimera that makes H₂in vivo

Abstract: Photosystem I-hydrogenase chimera intercepts electron flow directly from the photosynthetic electron transport chain and directs it to hydrogen production.

Cited by 62 publications

References 54 publications

Improved photosynthetic capacity and photosystem I oxidation via heterologous metabolism engineering in cyanobacteria

Improved photosynthetic capacity and photosystem I oxidation via heterologous metabolism engineering in cyanobacteria

Biosynthesis of the catalytic H-cluster of [FeFe] hydrogenase: the roles of the Fe–S maturase proteins HydE, HydF, and HydG

Green Catalysts: Applied and Synthetic Photosynthesis

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Rewiring photosynthesis: a photosystem I-hydrogenase chimera that makes H2in vivo

Abstract: Photosystem I-hydrogenase chimera intercepts electron flow directly from the photosynthetic electron transport chain and directs it to hydrogen production.

Cited by 62 publications

References 54 publications

Improved photosynthetic capacity and photosystem I oxidation via heterologous metabolism engineering in cyanobacteria

Improved photosynthetic capacity and photosystem I oxidation via heterologous metabolism engineering in cyanobacteria

Biosynthesis of the catalytic H-cluster of [FeFe] hydrogenase: the roles of the Fe–S maturase proteins HydE, HydF, and HydG

Green Catalysts: Applied and Synthetic Photosynthesis

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Rewiring photosynthesis: a photosystem I-hydrogenase chimera that makes H₂in vivo