RF heating of magnetic nanoparticles improves the thawing of cryopreserved biomaterials

Etheridge, Michael L.; Xu, Yansheng; Rott, Leoni; Choi, Jeunghwan; Glasmacher, Birgit; Bischof, John C.

doi:10.1142/s2339547814500204

Cited by 101 publications

(140 citation statements)

References 57 publications

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“…Previously, we utilized RF excited magnetic nanoparticles within 1 mL cylindrical vials to demonstrate the possibility of nanowarming in non-biological systems with standard cryoprotectants (3). Nanowarming is dependent on heat generation from iron oxide nanoparticles (IONPs) that can be excited in a RF field.…”

Section: Resultsmentioning

confidence: 99%

“…Our study builds on previous low volume physical (3) and theoretical work (4) to demonstrate that nanoparticle heating can improve tissue viability and prevent physical failure during warming after cryopreservation of larger volumes compared to the gold standard, convection. The ability of metallic nanoparticles to transduce radiofrequency (RF) and light energy for cancer therapy is already well established (5, 6).…”

Section: Introductionmentioning

confidence: 85%

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Improved tissue cryopreservation using inductive heating of magnetic nanoparticles

et al. 2017

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Vitrification, a kinetic process of liquid solidification into glass, poses many potential benefits for tissue cryopreservation including indefinite storage, banking, and facilitation of tissue matching for transplantation. To date, however, successful rewarming of tissues vitrified in VS55, a cryoprotectant solution, can only be achieved by convective warming of small volumes on the order of one mL. Successful rewarming requires both uniform and fast rates in order to reduce thermal mechanical stress and cracks, and to prevent rewarming phase crystallization. Here we present a scalable nanowarming technology for 1 to 80 mL samples using radiofrequency-excited mesoporous silica coated iron oxide nanoparticles in VS55. Advanced imaging including Sweep Imaging with Fourier Transform and micro computed tomography were used to verify loading and unloading of VS55 and nanoparticles and successful vitrification of human dermal fibroblast cells, porcine arteries, and porcine aortic heart valve leaflet tissue. Nanowarming was then used to demonstrate uniform and rapid rewarming at > 130 °C/min in both physical (1 to 80 mL) and biological systems (1 to 50 mL). Nanowarming yielded viability that matched control and/or exceeded gold standard convective warming in 1 to 50 mL systems, and improved viability compared to slow warmed (crystallized) samples. Finally, biomechanical testing displayed no significant biomechanical property changes in blood vessel length or elastic modulus after nanowarming compared to untreated fresh control porcine arteries. In aggregate, these results demonstrate new physical and biological evidence that nanowarming can improve the outcome of vitrified cryogenic storage of tissues in larger sample volumes.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 85%

Improved tissue cryopreservation using inductive heating of magnetic nanoparticles

et al. 2017

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“…Thus, magnetic fluid hyperthermia continues to be of considerable interest for cancer treatment and controlled warming of vitrified systems for regenerative medicine applications [266][267][268]. For this reason, it is imperative to identify biocompatible, reproducible, and high magnetic heating particles.…”

Section: Magnetic Nanoparticle Heatingmentioning

confidence: 99%

Evaluating Broader Impacts of Nanoscale Thermal Transport Research

Shi

Dames

Lukes

et al. 2015

Nanoscale and Microscale Thermophysical Engineering

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The past two decades have witnessed the emergence and rapid growth of the research field of nanoscale thermal transport. Much of the work in this field has been fundamental studies that have explored the mechanisms of heat transport in nanoscale films, wires, particles, interfaces, and channels. However, in recent years there has been an increasing emphasis on utilizing the fundamental knowledge gained toward understanding and improving Manuscript 128 L. SHI ET AL.device and system performances. In this opinion article, an attempt is made to provide an evaluation of the existing and potential impacts of the basic research efforts in this field on the developments of the heat transfer discipline, workforce, and a number of technologies, including heat-assisted magnetic recording, phase change memories, thermal management of microelectronics, thermoelectric energy conversion, thermal energy storage, building and vehicle heating and cooling, manufacturing, and biomedical devices. The goal is to identify successful examples, significant challenges, and potential opportunities where thermal science research in nanoscale has been or will be a game changer.

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“…4) [148] – until recently having used nanowarming, which takes advantage of the ability of metallic nanoparticles to transform a radio frequency or “light energy” into heat [149], as evidenced by a report in Science Translational Medicine from the Bischof group [137]. In that article, the authors demonstrated their scalable and biocompatible nanowarming technology using radio frequency-excited iron oxide nanoparticles (IONPs) to uniformly warm large vitrified volumes (up to 80 mL) at over 100°C/min and avoid (a) ice crystallization and (b) fracturing while (c) decreasing the total concentration of toxic CPAs needed [137].…”

Section: Bioengineering Applicationsmentioning

confidence: 99%

New Approaches to Cryopreservation of Cells, Tissues, and Organs

Taylor¹,

Weegman²,

Baicu³

et al. 2019

Transfus Med Hemother

131

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In this concept article, we outline a variety of new approaches that have been conceived to address some of the remaining challenges for developing improved methods of biopreservation. This recognizes a true renaissance and variety of complimentary, high-potential approaches leveraging inspiration by nature, nanotechnology, the thermodynamics of pressure, and several other key fields. Development of an organ and tissue supply chain that can meet the healthcare demands of the 21st century means overcoming twin challenges of (1) having enough of these lifesaving resources and (2) having the means to store and transport them for a variety of applications. Each has distinct but overlapping logistical limitations affecting transplantation, regenerative medicine, and drug discovery, with challenges shared among major areas of biomedicine including tissue engineering, trauma care, transfusion medicine, and biomedical research. There are several approaches to biopreservation, the optimum choice of which is dictated by the nature and complexity of the tissue and the required length of storage. Short-term hypothermic storage at temperatures a few degrees above the freezing point has provided the basis for nearly all methods of preserving tissues and solid organs that, to date, have proved refractory to cryopreservation techniques successfully developed for single-cell systems. In essence, these short-term techniques have been based on designing solutions for cellular protection against the effects of warm and cold ischemia and basically rely upon the protective effects of reduced temperatures brought about by Arrhenius kinetics of chemical reactions. However, further optimization of such preservation strategies is now seen to be restricted. Long-term preservation calls for much lower temperatures and requires the tissue to withstand the rigors of heat and mass transfer during protocols designed to optimize cooling and warming in the presence of cryoprotective agents. It is now accepted that with current methods of cryopreservation, uncontrolled ice formation in structured tissues and organs at subzero temperatures is the single most critical factor that severely restricts the extent to which tissues can survive procedures involving freezing and thawing. In recent years, this major problem has been effectively circumvented in some tissues by using ice-free cryopreservation techniques based upon vitrification. Nevertheless, despite these promising advances there remain several recognized hurdles to be overcome before deep-subzero cryopreservation, either by classic freezing and thawing or by vitrification, can provide the much-needed means for biobanking complex tissues and organs for extended periods of weeks, months, or even years. In many cases, the approaches outlined here, including new underexplored paradigms of high-subzero preservation, are novel and inspired by mechanisms of freeze tolerance, or freeze avoidance, in nature. Others apply new bioengineering techniques such as nanotechnology, isochoric pressure preserv...

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RF heating of magnetic nanoparticles improves the thawing of cryopreserved biomaterials

Cited by 101 publications

References 57 publications

Improved tissue cryopreservation using inductive heating of magnetic nanoparticles

Improved tissue cryopreservation using inductive heating of magnetic nanoparticles

Evaluating Broader Impacts of Nanoscale Thermal Transport Research

New Approaches to Cryopreservation of Cells, Tissues, and Organs

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