RFLP analysis and genomic in situ hybridization (GISH) in somatic hybrids and their progeny between Lycopersicon esculentum and Solanum lycopersicoides

Homoeologous pairing and recombination in Solanum lycopersicoides monosomic addition and substitution lines of tomato

“…Antheridia from young flower buds were fixed overnight in ethanol: acetic acid (3:1), then rinsed with distilled water and incubated in 0.01 M citrate buffer (0.01 M sodium citrate and 0.01 M citric acid, pH 4.5) for 5 min. To enhance chromosome spreading and morphology, the material was then incubated in an enzyme mixture consisting of 2% cellulase Onozuka RS (Yakult, Tokyo), 0.3% pectolyase (Sigma-aldrich) and 1.5% macerozyme R-200 (Yakult, Tokyo) in citrate buffer for 30 min at 37 (Escalante et al 1998). The partially digested antheridia were rinsed with distilled water, and individual anthers were subsequently placed on a clean slide and macerated in a drop of fresh 3:1 ethanol: acetic acid with a pair of forceps.…”

Protoplast fusion in the genus Gentiana: genomic composition and genetic stability of somatic hybrids between Gentiana kurroo Royle and G. cruciata L.

“…elimination of chloroplasts inherited from one of the fusion partners in hybrid cells was observed in most somatic hybrids (Walters and Earle 1993). Again, this process can be either random (Scowcroft and Larkin 1981;Lössl et al 1994;Escalante et al 1998) or biased (Levi et al 1988;Bonnema et al 1992;Buiteveld et al 1998), whereas the co-existence of chloroplasts from both parental species is very rare (Mohapatra et al 1998;Moreira et al 2000). Several factors have been proposed to influence biased chloroplast transmission, along with their possible modus operandi (Rose et al 1990 and references therein).…”

Protoplast Fusion Technology – Somatic Hybridization and Cybridization

“…Successful somatic hybridization in citrus rootstock improvement has allowed the creation of a rootstock breeding programme at the tetraploid level that achieves maximum genetic diversity in zygotic progeny and has great potential for controlling tree size [23]. Much of the excitement generated from somatic hybridization, has been the expanded opportunities for wide hybridization especially the production of intergeneric combinations that maximize genetic diversity [5,[24][25][26][27][28][29]. Many somatic hybrids have been produced to access genes that confer disease resistance [30,31].…”