RFLP mapping of the abp1 locus in maize (Zea mays L.)

Löbler, Marian; Hirsch, Ann M.

doi:10.1007/bf00019170

Cited by 9 publications

(2 citation statements)

References 10 publications

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“…Genomic DNA was isolated from M. truncatula or alfalfa leaves according to Cone (1989) with minor modifications (Lobler and Hirsch, 1990). Plasmid DNA was prepared according to established procedures (Maniatis et al, 1982).…”

Section: Nucleic Acid Isolation and Blottingmentioning

confidence: 99%

A Gene That Encodes a Proline-Rich Nodulin with Limited Homology to PsENOD12 Is Expressed in the Invasion Zone of Rhizobium meliloti-Induced Alfalfa Root Nodules

Löbler

Hirsch

1993

Plant Physiol.

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~ ~~~To define the early stages of the interaction between Rhizobium and host legumes, we have cloned and characterized three early nodulin-encoding sequences from an alfalfa (Medicago sativa 1.) cDNA library by probing with a fragment of a cDNA clone for PsENOD12, an infection-related nodulin from pea (Pisum sativum 1.). Although the coding regions of the three clones are 95 to 98% homologous to each other, they are only 43% homologous to the pea clone. However, the putative signal peptide encoded by the alfalfa cDNA clones is 100% homologous to the PsENOD12 signal peptide. The spatial and temporal expression patterns of PsENODl2 and the alfalfa clones were compared. In situ hybridization experiments detected RNA transcripts in the invasion zone of mature nitrogen-fixing nodules, the same site where PsENODl2 mRNAs are found. Transcripts were also found by in situ hybridization in cells of Rhizobium meliloti exoH mutant-induced nodules penetrated by infection threads, but northern analysis did not detect transcripts in Inf-(infection thread minus) nodules elicited by R. meliloti ex06 nodules or in pseudonodules elicited by treatment with the auxin transport inhibitor N-1-(naphthy1)phthalamic acid. In addition, the alfalfa gene represented by these cDNA clones exhibited a temporal expression pattern that differed from that of PsENOD12, which is transiently expressed. These data, plus information derived from Southern blot analysis, indicate that we have isolated cDNA clones for a novel early nodulin, which we have designated MsENODlO (Medicago sativa Early Nodulin 10).

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Section: Nucleic Acid Isolation and Blottingmentioning

confidence: 99%

A Gene That Encodes a Proline-Rich Nodulin with Limited Homology to PsENOD12 Is Expressed in the Invasion Zone of Rhizobium meliloti-Induced Alfalfa Root Nodules

Löbler

Hirsch

1993

Plant Physiol.

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“…Since then, other procedures of purification were developed (Shimomura et al 1986, Napier et al 1988). The ABP-1 eDNA was cloned, sequenced (Inohara et al 1989, Tillmann et al 1989, and mapped (L6bler and Hirsch 1990). The sequence had no homology to other sequences in data bases ).…”

Section: Introductionmentioning

confidence: 99%

Immunocytochemical localisation of auxin-binding proteins in coleoptiles and embryos ofZea mays L.

1998

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Summary. The auxin-binding protein ABP-1 was localisedimmunocytochemically in coleoptiles and immature embryos of Zea mays. Two primary polyclonal antibodies raised against ABP-1 and secondary antibodies were either labelled with FITC or 10 nm gold particles for light microscopy, and with 10 nm gold particles for transmission electron microscopy. Light microscopy revealed that ABP-1 was localised in the epidermal cells of etiolated maize coleoptiles, in subepidermal parenchymatic mesophyll cells of the coleoptile and in the companion cells of the vascular bundles. Most labelling was found in the cytoplasm, less in nuclei and vacuoles and cell walls appeared negative. The region of the plasma membrane exhibited prominent labelling. Embryos showed low labelling throughout their tissues just after excision, but after culture for 7 days intensive labelling was found in the epidermis of the scutellum. Quantitative electron microscopy confirmed that ABP-1 was present in the cytoplasm of epidermal, mesophyll, and companion cells of coleoptiles. Gold particles were neither found in cell walls nor in the cuticle. Areas with ER and dictyosomes within epidermal and mesophyll ceils of coleoptiles had a denser labelling with gold particles than elsewhere. Labelling at the plasma membrane, being the site where the auxin binds to the ABP, was observed at low levels in all cells examined, which is due to the method applied. Epidermal cells of embryos cultured for 5 days exhibited high levels of gold particles in ER and nuclei, and lower levels in the cytoplasm. The distribution is only partly in accordance with the model in which ABP is thought to cycle through the plant cell from the ER via the Golgi system towards the plasma membrane.

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The Auxin Receptor: Structure and Distribution

Napier¹,

Venis²

1992

Transport and Receptor Proteins of Plant Membranes

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RFLP mapping of the abp1 locus in maize (Zea mays L.)

Cited by 9 publications

References 10 publications

A Gene That Encodes a Proline-Rich Nodulin with Limited Homology to PsENOD12 Is Expressed in the Invasion Zone of Rhizobium meliloti-Induced Alfalfa Root Nodules

A Gene That Encodes a Proline-Rich Nodulin with Limited Homology to PsENOD12 Is Expressed in the Invasion Zone of Rhizobium meliloti-Induced Alfalfa Root Nodules

Immunocytochemical localisation of auxin-binding proteins in coleoptiles and embryos ofZea mays L.

The Auxin Receptor: Structure and Distribution

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