RFLP markers for sugar beet breeding: chromosomal linkage maps and location of major genes for rhizomania resistance, monogermy and hypocotyl colour

Abstract

SummaryAn RFLP linkage map for the nine chromosomes of sugar beet (Beta vulgaris L. ssp. vulgarisvar. altissima Doell) was constructed by using a segregating population from a cross between two plants which were heterozygous for several agronomically interesting characters. One hundred and eleven RFLP loci have been mapped to nine linkage groups using 92 genomic markers. The current RFLP map covers a total length of 540 cM. Evidence for the existence of a major gene for rhizomania resistance ( R r l ) is given, together with its map position on linkage group IV in the interval between loci GS44 and GS28a. The presence of an RFLP fragment at the GS3dlocus is, until now, the best molecular marker for rhizomania-resistant genotypes in segregating populations of sugar beet; GS3d is linked to Rrl with 6.7 cM. The gene MM, controlling the polygerm/monogerm seed type, has been mapped on linkage group IX in a distal position at 4.2 cM from the locus GS7. The gene Rcontrolling the hypocotyl colour maps to linkage group VII and does not recombine with the RFLP locus GS42. The inheritance of a group of RFLP loci revealed the possible presence of a translocation in the population used to establish the map. The data presented are discussed in relation to the possibility of using RFLP markers in sugar beet breeding.

Multiplexed, linkage group-specific SNP marker sets for rapid genetic mapping and fingerprinting of sugar beet (Beta vulgaris L.)

“…a: Barzen et al 1992, b: Barzen et al 1995, c: Schneider et al 1999, d: Schneider et al 2001, e: Schneider et al 2002, f: Hunger et al 2003; ": RFLP marker, ᮡ: marker developed from expressed gene related to carbohydrate metabolism and yield, *: marker developed from expressed RGA.…”

RFLP markers in the genus Beta: characterization of DNA sequences from a Beta vulgaris library

“…In sugar beet, two maps have been published to date (Barzen et al, 1992;Pillen et al, 1992). The construction of RFLP maps has primarily been motivated by their usefulness in the support of breeding programmes, and as a prerequisite for gene isolation and physical mapping.…”

Analysis of restriction fragment length polymorphism of Japanese sugar beet lines with incomplete monogermity.

“…Some aspects of the use of RFLP in plant genetics and breeding have been discussed earlier (Winter and Kahl, 1995). The method has been used to study the major genes of four morphological traits mapped on an RFLP map:annual habit (Boudry et al, 1994), rhizomania resistance, monogermity, and hypocotyl color (Barzen et al, 1992(Barzen et al, , 1995. The monogerm or multigerm gene M-m has been mapped on the fifth linkage group and is closely linked with the makers 7, 49, 117, and 238a (Barzen et al, 1995).…”