RFLP of the human placental alkaline phosphatase gene (PLAP)

Abstract

SOURCE AND DESCRIPTION OF CLONE: ALP-1, a 1.1 kb EcoRI/ApaI fragment of a PLAP cDNA subcloned into pBR322 from a XgtlO cDNA library of the cell line HEp-2 (Martin et al., 1987). POLYMORPHISM: RsaI identifies a two allele polymorphism with polymorphic bands at 1.6 kb and 1.8 kb as well as a constant band at 2.2 kb (Figure). TaqI reveals a

Correlation between Rsal Restriction Fragment Length Polymorphism and Electrophoretic Types of Human Placental Alkaline Phosphatase

“…The Southern blot revealed polymor phic bands at 1.6 and 1.8-kb as described earlier by Martin et al [14] and constant bands at 2.4, 2.2, 1.3, 1.2 and 1.1 kb. The three Rsal genotypes are shown in figure 1.…”

“…Table 1 shows the relationship between the Rsal genotypes and the electropho retic PLAP phenotypes. The frequencies of the 1.6 (Al)-and 1.8 (A2)-kb alleles were 0.46 and 0.54, respectively, which is not significantly different from the fre quencies (0.38 and 0.63) found by Martin et al [14] in 20 European Whites. The ob served distribution of Rsal genotypes showed no significant departure from the expected distribution assuming a Hardy-Weinberg equilibrium (x: = 2.54, p>0.05).…”

“…Restriction fragment length polymor phism (RFLP) has been reported for PLAP [12][13][14] and allele frequencies have been given for PstI [12], Rsal and TaqI [14]. Tsavaler et al [12] studied the rela tionship between PstI restriction patterns and electrophoretic PLAP phenotypes in a series of 27 placentae.…”

A New PstI Restriction Fragment Length Polymorphism (RFLP) of Placental Alkaline Phosphatase

“…Martin et al [14] found poly morphic DNA fragments after cleavage with Rsal. In a series of 20 European Whites the frequencies of the 1.6-kb (Al) and 1.8-kb (A2) alleles were 0.38 and 0.63, respectively.…”

Ethnic Differences in Enzyme and DNA Polymorphisms of Human Tissue-Specific Alkaline Phosphatases

“…By means of starch gel electrophore sis of placental extracts and histochemical staining, three common alleles (1,2 and 3 [or S. F and 1 in older nomenclature]) and a large number of rare alleles have been discovered [9][10][11]. Additional genetic variation has been found using immunochemical methods [12,13], and on the DNA level restriction fragment length polymorphisms (RFLPs) of PLAP have been found after cleavage with Pstl [14,15] and Rsal [16]. Genetic polymorphism has also been detected in GCAP using immunochem ical methods [17] and restriction enzyme anal ysis [18,19].…”