RGD-coated titanium implants stimulate increased bone formation in vivo

Ferris, Diana; Moodie, Geoff; Dimond, P.M.; Gioranni, C. W. D.; Ehrlich, Michael G.; Valentini, Robert F.

doi:10.1016/s0142-9612(99)00161-1

Cited by 263 publications

(175 citation statements)

References 55 publications

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“…Zreiqat et al 8) reported that human bone-derived cells on GRGDSP peptide-bound Ti surfaces displayed significantly increased levels of OC gene expression when compared to uncoated Ti. Furthermore, Ferris et al 6) found that RGD-coated Ti implants stimulated new bone formation in vivo, such that there was a significant increase in bone volume around Ti implants in rat femur after implantation. Additionally, the effect of RGD peptide coating of Ti implants in dog models was studied.…”

Section: Resultsmentioning

confidence: 99%

“…It has been pointed out that synthetic peptides with a functional domain consisting of an adhesive molecule may have some advantages in the development of biomaterial implants. This is because when compared to whole FN protein, a small synthetic peptide containing a functional domain wields the twofold advantages of less immunogenicity and lower cost of production from serum or recombinant gene clone 6) . In view of the above mentioned advantages, we designed and synthesized RGD-motif-containing peptides derived from collagen, laminin, FN, and BSP.…”

Section: Introductionmentioning

confidence: 99%

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Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide

et al. 2008

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Enhanced adhesion and migration of osteoblastic cells on titanium (Ti) surface is believed to increase the success rate of implant therapy. A GRGDSP peptide derived from fibronectin was coated on Ti surfaces using a tresyl chloride activation technique, and then MC3T3-E1 osteoblastic cells were cultured on the Ti surfaces. After 15 days, total RNA was isolated from the cells and gene expression level were analyzed by Affymetrix GeneChip system. The expression levels of many genes in MC3T3-E1 cells cultured on GRGDSP-coated Ti surface were altered when compared to uncoated Ti. In particular, the elevated mRNA levels of bone sialoprotein (BSP) and osteocalcin (OC) were successfully confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. In light of the results obtained, GRGDSP-coated Ti presented the potential of evolving into a useful biomaterial for successful implant therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide

et al. 2008

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“…The most commonly used peptide sequence for surface modification is the above mentioned cell adhesion motif RGD (113)(114)(115). Additionally, various other peptide sequences have been immobilized onto implant materials (Table III) (99,112,(116)(117)(118)(119)(120).…”

Section: Ecm Proteins and Peptide Sequence Immobilizationmentioning

confidence: 99%

“…via functional groups like hydroxyl-, amino-, or carboxyl groups. RGD-functionalized materials are reported to improve early bone ingrowth and matrix mineralization in implanted constructs (113,121) and to induce more bone contact to the implant (114,122). Fig.…”

Section: Ecm Proteins and Peptide Sequence Immobilizationmentioning

confidence: 99%

Organic–Inorganic Surface Modifications for Titanium Implant Surfaces

et al. 2008

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Abstract. This paper reviews current physicochemical and biochemical coating techniques that are investigated to enhance bone regeneration at the interface of titanium implant materials. By applying coatings onto titanium surfaces that mimic the organic and inorganic components of living bone tissue, a physiological transition between the non-physiological titanium surface and surrounding bone tissue can be established. In this way, the coated titanium implants stimulate bone formation from the implant surface, thereby enhancing early and strong fixation of bone-substituting implants. As such, a continuous transition from bone tissue to implant surface is induced. This review presents an overview of various techniques that can be used to this end, and that are inspired by either inorganic (calcium phosphate) or organic (extracellular matrix components, growth factors, enzymes, etc.) components of natural bone tissue. The combination, however, of both organic and inorganic constituents is expected to result into truly bone-resembling coatings, and as such to a new generation of surface-modified titanium implants with improved functionality and biological efficacy.

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“…These findings show that RGD separated from P-RGD after ultrasonic rinsing with MilliQ water, leaving only S(PO3H2)PA bonded to the Ti surface. The degree of immobilization of RGD on Ti surfaces achieved in previous studies [12][13][14]17) has been measured under relatively static conditions. In future studies, it will be necessary to measure the degree of RGD immobilization by irrigating the modified surface dynamically and ultrasonically.…”

Section: Resultsmentioning

confidence: 99%

Degree of immobilization of synthetic RGDS(PO3H2)PA peptides on titanium surfaces

et al. 2010

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The purpose of this study was to characterize the chemical interaction between titanium surfaces and the peptide RGDS(PO3H2)PA (P-RGD) synthesized from RGD peptide (RGD) and o-phospho-L-serine (P-Ser), and to determine the degree of peptide immobilization on the titanium surface. X-ray photoelectron spectroscopy showed that the adsorption amount of RGD was significantly smaller than those of P-Ser and P-RGD (p<0.05). Furthermore, although it appeared that P-RGD bonded to the surface, ultrasonic rinsing with water caused it to dissociate, releasing RGD and leaving only S(PO3H2)PA bonded to the surface. These findings show that although it remains difficult to obtain a stable P-RGD layer, the phosphate functional group greatly improves immobilization of the molecule on titanium surfaces.

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RGD-coated titanium implants stimulate increased bone formation in vivo

Cited by 263 publications

References 55 publications

Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide

Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide

Organic–Inorganic Surface Modifications for Titanium Implant Surfaces

Degree of immobilization of synthetic RGDS(PO3H2)PA peptides on titanium surfaces

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