Rgl-exomiR-7972, a novel plant exosomal microRNA derived from fresh Rehmanniae Radix, ameliorated lipopolysaccharide-induced acute lung injury and gut dysbiosis

Exploring new avenues of health protection: plant-derived nanovesicles reshape microbial communities

“… Tartary buckwheat UC TBDNs significantly promoted E. coli and LGG , enhanced fecal microbial diversity, and increased short-chain fatty acid levels Human E.coli and LGG A total of 129 microRNAs were identified by sequencing analyses, including 11 newly discovered microRNAs. Target gene prediction indicated that some of the microRNAs were able to target functional genes in physiological processes related to E. coli and LGG [ 43 ] Tomato UC Promoting the growth of probiotic LGG while inhibiting the growth of the conditional pathogens C. difficile and F. nucleatum Smulator of intestinal microbial ecosystem model LGG ; C. diff and F. nucleatum The antimicrobial effect of tomato-derived nanovesicles is driven by the presence of specific lipids in the nanovesicles, as demonstrated by lipid depletion and reconstitution experiments [ 14 ] Bee pollen UC Inhibition of S. aureus biofilm formation – S. aureus – [ 67 ] Rehmannia UC + D Antimicrobial, inhibits biofilm formation LPS-treated mice E.coli miR-7972 down-regulates the expression of GPR 161, activates the Hedgehog pathway, and inhibits biofilm formation in E. coli by targeting the virulence gene stx 2 [ 44 ] Garlic UC + D Regulation of microbial community homeostasis DSS-induced gut microbiota dysbiosis – – [ 16 ] Ginger UC + D Regulation of microbial community homeostasis C57 BL/6 mouse/human …”

“…Conversely, Qiu et al found that 12 miRNAs were present in Remannia-derived nanovesicles, among which miR-7972 had the highest abundance. They found that miR-7972 could bind to the bacterial virulence gene shiga toxin 2 (stx2), thereby inhibiting the growth of Escherichia coli [ 44 ]. These studies show that different PDNVs exert apparently different effects due to differences in their components.…”

“…miR-7972 enriched in Remannia-derived nanovesicles may exert an inhibitory effect on the expression of the virulence gene Stx2 caused by Escherichia coli . This not only reduced the growth of Escherichia coli but also inhibited its biofilm formation [ 44 ]. Reduced biofilm formation is important for the effective application of antibiotics, as antibiotics become less effective against biofilms.…”

“…4 B, C). Also, Qiu et al found that Remannia-derived nanovesicles might enhance glycerolipid metabolism, cytoskeletal proteins, β-alanine metabolism and lipid metabolism in bacteria and inhibit nitrogen metabolism, folate biosynthesis, and cancer pathways in the gut microbiota [ 44 ]. But these may be based on data analyses that need more data to confirm.…”

Dipsacus Asperoides-Derived Exosomes-Like Nanoparticles Inhibit the Progression of Osteosarcoma via Activating P38/JNK Signaling Pathway

“… 5 Plant exosomal miRNAs extracted from fresh rehmannia can reduce LPS-induced acute lung injury in mice. 6 Xia et al showed that exosomal microRNA-31, derived from halofuginone, inhibits breast cancer cell proliferation and cell cycle mediation during tumorigenesis. 7 Taken together, plant exosome nanoparticles have been found to be beneficial in multiply diseases.…”