rGO nanomaterial-mediated cancer targeting and photothermal therapy in a microfluidic co-culture platform

Abstract

We developed the microfluidic co-culture platform to study photothermal therapy applications. We conjugated folic acid (FA) to target breast cancer cells using reduced graphene oxide (rGO)-based functional nanomaterials. To characterize the structure of rGO-based nanomaterials, we analyzed the molecular spectrum using UV-visible and Fouriertransform infrared spectroscopy (FT-IR). We demonstrated the effect of rGO-FA-based nanomaterials on photothermal therapy of breast cancer cells in the microfluidic co-culture platform. From the microfluidic co-culture platform with breast cancer cells and human umbilical vein endothelial cells (HUVECs), we observed that the viability of breast cancer cells treated with rGO-FA-based functional nanomaterials was significantly decreased after near-infrared (NIR) laser irradiation. Therefore, this microfluidic co-culture platform could be a potentially powerful tool for studying cancer cell targeting and photothermal therapy.

Near-Infrared Light-Triggered Thermo-responsive Poly(N-Isopropylacrylamide)-Pyrrole Nanocomposites for Chemo-photothermal Cancer Therapy

“…2c). While the spectra of PNIPAM-AAc and PNIPAM-ppy (without FA) did not show any absorption peak, PNI-PAM-ppy-FA showed the additional peak at 280 nm, which was distinctive in FA [32]. This result supported that FA molecules were grafted on PNIPAM-based nanocomposites.…”

“…Since LCST of PNIPAM-ppy-FA decreased slightly to 42 °C, the cumulative release at body temperature was found to be relative higher than room temperature. A large amount of Dox was released, however, the released Dox did not affect the therapeutic efficiency, because Dox@PNIPAM-ppy-FA nanocomposites could be internalized in only folate-receptor positive cells, as previously described [26,32]. At above LCST (50 °C), the amount of Dox from PNIAPM-ppy-FA nanocomposites was released four times higher than room temperature, suggesting that PNIPAM-ppy-FA nanocomposites could be used in controlled drug release system through thermal response.…”

Microfluidic System to Analyze the Effects of Interleukin 6 on Lymphatic Breast Cancer Metastasis

“…The microchannel structure of the LTB chip was fabricated using the replica molding technique. First, we designed the device in silico using a computer-aided design (CAD) program (Mun et al, 2020 ). We created a positive relief 100 μm pattern on a silicon wafer by cleaning and dehydrating a 6-inch wafer and coating it uniformly with SU-8, the photoresist, using a spin coater.…”

Probing mechanobiological role of filamin A in migration and invasion of human U87 glioblastoma cells using submicron soft pillars

“…Photolithography with reactive ion etching (RIE) was used to fabricate a mold of linearly arranged arrays of holes (900 nm, diameter; 2 μm, depth; and 1.8 μm, c-t-c distance between holes) over 5-inch silicon wafers. PDMS Sylgrad ® 184 silicon elastomeric base (Dow Corning Co.) [21] was mixed with its curing agent at either a 5:1 or 10:1 (w/w) ratio to obtain PDMS elastic modules of 4 and 2 MPa [22] and the mixture was degassed for 15 min. Next, the degassed mixture was spin-coated over the mold at 500 rpm for 10 s and subsequently at 1,800 rpm for 30 s before being degassed for 30 min to remove any trapped bubbles from the coated layer.…”