RH genotypes among Malaysian blood donors

Musa, Rozi Hanisa; Hassan, Afifah; Ayob, Yasmin; Yusoff, Narazah Mohd

doi:10.5372/1905-7415.0804.319

Cited by 1 publication

(3 citation statements)

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“…Rh system antigens had the highest mismatches (80.4%) among all 4 blood group systems in the present study with highest discrepancies found for E antigen (46/112 samples) as shown in Table 1. Prevalence E [14]. Similar findings were reported by Reid et al as one of E + samples had mixed field reaction in e typing and genotyped as RHE/RHE [16].…”

Section: Discussionsupporting

confidence: 86%

“…After completion of cross match procedures remaining EDTA samples were used for serological phenotyping of Rh, Kell, Duffy and Kidd system antigens and genotyping by molecule methods for the same antigens. Serological investigations were done by tube agglutination technique using conventional antisera for particular antigens from IMMUCOR, INC, U.S. Molecular investigation of Rh system antigens were carried out using allele specific PCR for RHCc [13,14] and RHEe [15] alleles and for investigating antigens of Kell, Duffy and Kidd antigens PCR-RFLP techniques were used [16]. Two ml of EDTA and 3 ml of clotted blood was taken from the recipients for extended grouping and cross matching just before next transfusion was due.…”

Section: Methodsmentioning

confidence: 99%

“…The extracted DNA was then analyzed for quality on 0.8% agarose gel and quantitation by nanospectrophometry (IMPLAN, Germany) at a wavelength of 260/280 nm before molecular analysis was performed. a Molecular genotyping of Rh blood group system b RhC and Rhc allele identification by multiplex PCR [13,14]. 8 pairs of primers were used for multiplex PCR to detect presence of RHD exon 7 and intron 4 and RHC and RHc alleles.…”

Section: Molecular Investigationsmentioning

confidence: 99%

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Comparison of serology and molecular detection of common red cell antigens in multitransfused thalassemia major and sickle cell disease patients

Shah

Patel

et al. 2020

Transfusion and Apheresis Science

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Background: Serological testing for extended RHCcEe, Kell, Kidd and Duffy blood grouping from multitransfused patients may not give correct blood grouping of the recipient. Hence molecular testing for these blood groups was compared with serological groups in a cohort of multitransfused thalassemia mjor and sickle cell anaemia patients. Objective: Molecular genotyping of antigens of Rh (D, C, c, E, e), Kell (K, k), Duffy (Fy a , Fy b) and Kidd (Jk a , Jk b) blood group antigens by PCR and PCR-RFLP methods and comparison of predicted genotypes with their serological phenotypes. Materials and methods: A cohort of multitransfused thalassemia and sickle cell anemia patient were serologically and molecularly tested for RHCc, RHEe, K, k Fy a , Fy b , Jk a and Jk b antigens and compared. Serological testing was done by tube agglutination and molecular testing was done either by allele specific PCR or by RFLP technique just before next transfusion. Results: In more than 80% of the cases recipient's molecular testing blood groups were at variance with serologically tested blood groups (p < 0.0001). Mixed field reactions in serological typing were common. In sickle cell anemia patients no discrepancy was found. Molecular technique results were checked by Sanger's sequencing. Discussion: Extended phenotyping in multitransfused thalassemia patients by serological technique often donot detect the exact red cell phenotype of the recipient and molecular techniques for such grouping is preferable, especially in multitransfused thalassemia patients where red cells from previous transfusions continues to be present in significant numbers whenever the testing is done.

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Section: Discussionsupporting

confidence: 86%