Rhamnolipid biosurfactant adsorption on a plasma-treated polypropylene surface to induce antimicrobial and antiadhesive properties

Hajfarajollah, Hamidreza; Mehvari, Saeid; Habibian, Mahmoud; Mokhtarani, Babak; Noghabi, Kambiz Akbari

doi:10.1039/c5ra01233c

Cited by 45 publications

(23 citation statements)

References 26 publications

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“…However, published studies are mainly focused on the evaluation of antimicrobial activity on planktonic cells [69,70]. A limited number of studies addressed the potential of rhamnolipid biosurfactants in preventing microbial adhesion and bio lm formation on conditioned surfaces [41,[71][72][73][74], and to our knowledge, this is the rst time that an anti-bio lm rhamnolipid-coating was applied on medical-grade titanium.…”

Section: Discussionmentioning

confidence: 99%

Rhamnolipid Coating Reduces Microbial Biofilm Formation on Titanium Implants: an in-vitro Study

Tambone

Bonomi

Ghensi

et al. 2020

Preprint

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Background: Peri-implant mucositis and peri-implantitis are biofilm-related diseases causing major concern in oral implantology, requiring complex anti-infective procedures or implant removal. Microbial biosurfactants emerged as new of anti-biofilm agents for coating implantable devices preserving biocompatibility. This study aimed to assess the efficacy of rhamnolipid biosurfactant R89 (R89BS) to reduce Staphylococcus aureus and Staphylococcus epidermidis biofilm formation on titanium. Methods: R89BS was physically adsorbed on titanium discs (TDs) and the ability of coated TDs to inhibit biofilm formation was evaluated by quantifying biofilm biomass and cell metabolic activity, at different time-points, with respect to uncoated controls. A qualitative analysis of sessile cells was also performed by scanning electron microscopy. Results: R89BS-coated discs showed no cytotoxic effects on normal lung fibroblasts (MRC5). TDs coated with 4 mg/mL R89BS inhibited the biofilm biomass of S. aureus by 98%, 49% and 10% and of S. epidermidis by 53%, 29%, and 10% at 24, 48 and 72 h respectively. A significant reduction of the biofilm metabolic activity was also documented. The same coating applied on three commercial implant surfaces resulted in a biomass inhibition higher than 90% for S. aureus, and up to 75% for S. epidermidis at 24 h. Conclusions: R89BS-coating was effective in reducing Staphylococcus biofilm formation at the titanium implant surface. The anti-biofilm action can be obtained on several different commercially available implant surfaces, independently of their surface morphology.

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Section: Discussionmentioning

confidence: 99%

Rhamnolipid Coating Reduces Microbial Biofilm Formation on Titanium Implants: an in-vitro Study

Tambone

Bonomi

Ghensi

et al. 2020

Preprint

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“…1) [10,17]. There is a clear consensus on the antimicrobial and anti-adhesive properties potential displayed by the more commonly investigated biosurfactants, such as rhamnolipids and sophorolipids, tested against both planktonic and biofilm physiologies [9,[18][19][20]. In this work, the antimicrobial effects of MELs on Staphylococcus aureus biofilms were identified; having a positive impact on the disruption of biomass, biofilm metabolic activity and relative biofilm oxygen uptake.…”

Section: Introductionmentioning

confidence: 95%

Production of Mannosylerythritol Lipids (MELs) to be Used as Antimicrobial Agents Against S. aureus ATCC 6538

et al. 2020

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Antimicrobial resistance (AMR) is a current major health issue, both for the high rates of resistance observed in bacteria that cause common infections and for the complexity of the consequences of AMR. Pathogens like Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Mycobacterium tuberculosis among others are clear examples of antibiotic-resistant threats. Biosurfactants have recently emerged as a potential new generation of anti-adhesive and anti-biofilm agents; mannosylerythritol lipids (MELs) are biosurfactants produced by a range of fungi. A range of structural variants of MELs can be formed and the proportion of each isomer in the fermentation depends on the yeast used, the carbon substrate used for growth and the duration of the fermentation. In order to allow assessment of the possible functions of MELs as antimicrobial molecules, small quantities of MEL were produced by controlled fermentation. Fermentations of the yeast Pseudozyma aphidis using rapeseed oil as a carbon source yielded up to 165 g MELs /kg Substrate. The MELs formed by this strain was a mixture of MEL-A, MEL-B, MEL-C and MEL-D. The MELs produced were tested against S. aureus ATCC 6538 on pre-formed biofilm and on co-incubation biofilm experiments on silicone discs; showing a disruption of biomass, reduction of the biofilm metabolic activity and a bacteriostatic/bactericidal effect confirmed by a release of oxygen uptake p O 2 , the reduction of citrate synthase activity and scanning electron microscopy. The results show that MELs are promising antimicrobial molecules for biomedical technological applications that could be studied in detail in large-scale systems and in conjunction with animal tissue models.

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“…S.aureus, B. subtilis and K. pneumonia, in food and pharmaceutical industries. 31 Corrosion never stops but its scale and severity could be reduced. One of the best known methods for protection against corrosion is to use biocide.…”

Section: Antibacterial Effects Of Oil-cell-free Fermentation Brothmentioning

confidence: 99%

“…Additionally, rhamnolipid as a promising biosurfactant has been investigated to have the antibacterial capability in the areas of food and healthcare. [30][31][32][33][34] Its surfaceactive compound has also been tested for very good antiadhesive properties to prevent contamination of stainless steel surfaces by different pathogenic bacteria. 31 However, there is hardly a literature reporting about the effects of environmental biosurfactant (rhamnolipid) on the most harmful bacteria corroding the steel on oileld such as sulphatereducing bacteria (SRB) and saprophytic bacteria (TGB).…”

Section: Introductionmentioning

confidence: 99%

Corrosion of carbon steel induced by a microbial-enhanced oil recovery bacterium Pseudomonas sp. SWP-4

et al. 2017

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Rhamnolipid biosurfactant adsorption on a plasma-treated polypropylene surface to induce antimicrobial and antiadhesive properties

Abstract: A glycolipid type of biosurfactant (rhamnolipid), which is obtained from Pseudomonas aeruginosa MA01, was adsorbed on a polypropylene film to produce an antimicrobial and antiadhesive polymeric surface for the first time.

Cited by 45 publications

References 26 publications

Rhamnolipid Coating Reduces Microbial Biofilm Formation on Titanium Implants: an in-vitro Study

Rhamnolipid Coating Reduces Microbial Biofilm Formation on Titanium Implants: an in-vitro Study

Production of Mannosylerythritol Lipids (MELs) to be Used as Antimicrobial Agents Against S. aureus ATCC 6538

Corrosion of carbon steel induced by a microbial-enhanced oil recovery bacterium Pseudomonas sp. SWP-4

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