Rhamnolipid Surfactants: An Update on the General Aspects of These Remarkable Biomolecules

Nitschke, Márcia; Costa, Siddhartha G. V. A. O.; Contiero, Jonas

doi:10.1021/bp050239p

Cited by 269 publications

(191 citation statements)

References 68 publications

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“…The increasing ecological concern with using synthetic chemical surfactants has led us to propose rhamnolipid as environmentally benign substitute, although it will be necessary to reduce production costs. The use of renewable low-cost substrates, such as plant oil and grain starch, and even lignocellulosic biomass, could dramatically increase the economics of rhamnolipid production (Nitschke et al, 2005;Mukherjee et al, 2006). Many bacteria, especially Pseudomonads, can utilize efficiently renewable low-cost substrates, but they either lack the ability to biosynthesize the rhamnolipid, or only have very low yield of rhamnolipid.…”

Section: Technical Barriers and Need Of Our Researchmentioning

confidence: 99%

Bio-Engineering High Performance Microbial Strains for MEOR

Fang¹,

Wang²,

Shuler³

2007

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The main objectives of this three-year research project are: 1) to employ the latest advances in genetics and bioengineering, especially Directed Protein Evolution technology, to improve the effectiveness of the microbial enhanced oil recovery (MEOR) process. 2) to improve the surfactant activity and the thermal stability of bio-surfactant systems for MEOR; and 3) to develop improved laboratory methods and tools that screen quickly candidate bio-systems for EOR.Biosurfactants have been receiving increasing attention as Enhanced Oil Recovery (EOR) agents because of their unique properties (i.e., mild production conditions, lower toxicity, and higher biodegradability) compared to their synthetic chemical counterparts. Rhamnolipid as a potent natural biosurfactant has a wide range of potential applications, including EOR and bioremediation.During the three-year of the project period, we have successfully cloned the genes involved in the rhamnolipid bio-synthesis. And by using the Transposon containing Rhamnosyltransferase gene rhlAB, we engineered the new mutant strains P. aeruginosa PEER02 and E. coli TnERAB so they can produce rhamnolipid biosurfactans. We were able to produce rhamnolipds in both P. aeroginosa PAO1-RhlA -strain and P. fluorescens ATCC15453 strain, with the increase of 55 to 175 fold in rhamnolipid production comparing with wild type bacteria strain. We have also completed the first round direct evolution studies using Error-prone PCR technique and have constructed the library of RhlAB-containing Transposon to express mutant gene in heterologous hosts. Several methods, such as colorimetric agar plate assay, colorimetric spectrophotometer assay, bioactive assay and oil spreading assay have been established to detect and screen rhamnolipid production.Our engineered P. aeruginosa PEER02 strain can produce rhamnolipids with different carbon sources as substrate. Interfacial tension analysis (IFT) showed that different rhamnolipids from different substrates gave different performance. Those rhamnolipids with plant oil as substrateshowed as low an IFT as 0.05mN/m in the buffer solution with pH5.0 and 2% NaCl. Core flooding tests showed that rhamnolipids produced by our engineered bacteria are effective agents for EOR.At 250ppm rhamnolipid concentration from P. aeruginosa PEER02, 42% of the remaining oil after waterflood was recovered. These results were therefore significant towards considering the exploration of the studied rhamnolipids as EOR agents.2

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Section: Technical Barriers and Need Of Our Researchmentioning

confidence: 99%

Bio-Engineering High Performance Microbial Strains for MEOR

Fang¹,

Wang²,

Shuler³

2007

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“…Moreover, the reuse of wastes can represent a sustainable solution to the lack of raw material to be used for the production of energy, fuel and chemicals than can be integrated again in the industry. The production of high added value materials as bioproducts, nanomaterials and bio-polymers starting from wastes underlines, even more, the interest for their reutilization [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Valorization of tannery wastes: Lipoamino acid surfactant mixtures from the protein fraction of process wastewater

Bautista

Pérez

Garcı́a

et al. 2015

Chemical Engineering Journal

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The first stages of the transformation process of hides into leather (beamhouse process) generate an important waste in the tanning industry, since a considerable fraction of solubilised proteins ends up in waste water with the corresponding increase in contamination parameters, especially when the process is carried out without hair recovery (hair-pulping process). The objective of this work was the valorisation of this waste (the separated protein fraction) which conveniently hydrolyzed to amino acid level constituted the starting material for the production of biodegradable surfactants.The lipoamino acid surfactants were obtained by acylation of the amino acids from the protein hydrolisate. These surfactants were characterized and their physico-chemical and biological properties evaluated. They exhibit very low cmc values (about 40 mg/L).These surfactants are readily biodegradable and present an aquatic toxicity significantly lower than many common commercial surfactants derived whether from renewable or petrochemical feedstock. The mixtures of surfactants obtained are able to form oil/water emulsions that remain stable for at least one year. The results obtained in this work confirmed that it is possible the production of biodegradable and efficient lipoamino acid surfactant mixtures from the protein fraction present in beamhouse process wastewaters. This study constitutes a promising approach for the reduction of the pollution load from industrial tannery wastes and its valorisation as raw material for the production of surfactants with excellent environmental properties and good technical properties.

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“…Biosurfactants become more and more popular in soil remediation applications. Biosurfactants, which are produced by a variety of microorganisms (also those, used in bioremediation), show better than synthetic surfactants environmental biodegradability and higher activity at extreme temperature, pH and salinity [5,6]. At the present time, biosurfactants are widely used with the great advantage of bioremediation processes [7].…”

Section: Introductionmentioning

confidence: 99%

Wetting properties of biosurfactant (rhamnolipid) with synthetic surfactants mixtures in the context of soil remediation

Hallmann¹,

Mędrzycka²

2015

Annales UMCS, Chemia

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Wetting properties of synthetic surfactant Rokanol NL6, biosurfactant JBR 425 and their mixture have been investigated. On the basis of these investigations, the ability of used surfactants to remove the synthetic base oil (PAO6) from sandy soil and clay loam was evaluated. Surfactant solutions were applied for soil flushing in batch experiments. The results show that synthetic surfactant addition worsens physicochemical properties of pure biosurfactant but exhibits much higher oil removal efficiency than biosurfactant does.

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Rhamnolipid Surfactants: An Update on the General Aspects of These Remarkable Biomolecules

Cited by 269 publications

References 68 publications

Bio-Engineering High Performance Microbial Strains for MEOR

Bio-Engineering High Performance Microbial Strains for MEOR

Valorization of tannery wastes: Lipoamino acid surfactant mixtures from the protein fraction of process wastewater

Wetting properties of biosurfactant (rhamnolipid) with synthetic surfactants mixtures in the context of soil remediation

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