RHD genotyping and its implication in transfusion practice

Sassi, Awatef; Ouchari, Mouna; Houissa, B.; Romdhane, H. Ben; Abdelkefi, S.; Chakroun, Tahar; Yacoub, S.

doi:10.1016/j.transci.2014.10.019

Cited by 7 publications

(8 citation statements)

References 25 publications

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“…These genetic backgrounds of RhD-negative are different from the Iranian population located in the Middle East, while the frequency of our results is more similar to the European population [ 21 ]. The frequency of D-negative phenotype with a non-deletion RHD gene allele is approximately 0.6% in Caucasians, 10% in black Africans and 30% in Asians [ 2 , 22 ]. In our study, non-deletion RHD gene allele was found in 2 (1%) D-negative donors, indicating more consistency with the Caucasian population.…”

Section: Discussionmentioning

confidence: 99%

“…In our study, the RHD gene deletion had the highest association with the D−C−E−c+e+ phenotype (92.92%) and 87.5% of the C+/E+ donors had RHD gene deletion alleles. The frequency of D-negative phenotype with the presence of RHD gene non-deletion alleles in association with C and E antigens has also been reported [ 2 , 11 ]. Both donors with non-deletion allele in this study were positive for C antigen.…”

Section: Discussionmentioning

confidence: 99%

“…The antigen D is the most important and immunogenic antigen of the Rh blood group [ 1 , 2 ]. The proper identification of RhD antigen has a high clinical significance, and can play an important role in preventing the occurrence of hemolytic anemia in patients and newborns [ 2 ]. Serology is the standard method for detection of RhD antigen, but has limitations.…”

Section: Introductionmentioning

confidence: 99%

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RHD Genotyping by Molecular Analysis of Hybrid Rhesus box in RhD-Negative Blood Donors from Iran

Khosroshahi

Oodi

Namjou

et al. 2018

Indian J Hematol Blood Transfus

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D antigen is the most important and immunogenic antigen of the Rh blood group. The RhD-negative phenotype has different genetic backgrounds with variable distribution in different populations. Hybrid Rhesus box , resulting from RHD gene deletion, is used in genotyping studies of the Rh blood group as a marker to identify the RHD gene deletion. This study for the first time identified genetic mechanisms for the occurrence of RhD-negative phenotype among the Iranian population. 200 RhD-negative blood donors were randomly selected from Tehran Blood Transfusion Center. The phenotype of D, C, Ε, e and c antigens was serologically identified, and DNA was extracted from buffy coat. The molecular analysis of hybrid Rhesus box was performed by PCR-SSP and PCR-RFLP. Moreover, the presence of different exons of RHD gene was investigated by real-time PCR on extracted DNA. Hybrid Rhesus box was detected in all samples, and PCR-RFLP confirmed that 198 (99%) were homozygous for an RHD gene deletion and 2 were heterozygous for hybrid Rhesus box in which one (0.5%) had a weak D type 11 and the other one (0.5%) had a RHD - CE (2 - 9) - D 2 hybrid allele. Similar to Caucasians, the frequency of RHD gene deletion was high among the Iranian population studied in this investigation, so hybrid Rhesus box can be used as an efficient marker to detect RHD gene deletion in our population.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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RHD Genotyping by Molecular Analysis of Hybrid Rhesus box in RhD-Negative Blood Donors from Iran

Khosroshahi

Oodi

Namjou

et al. 2018

Indian J Hematol Blood Transfus

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“…5 D antigen variants have been studied at the DNA level in any Arab population since 2009 only, notably in Gaza, 6 Tunisia, [7][8][9][10][11][12][13][14][15][16][17][18][19] Egypt, [20][21][22] and Libya. 23 The molecular causes of the serologic D-, 7,9,15,16 weak D, 7,[10][11][12][13][15][16][17][18] and partial D phenotypes 7,10,[17][18][19] have been evaluated in different sets of Tunisian samples ranging from 100 to 2000 samples each. Similar to Caucasian populations, 24,25 the prevalence of individuals carrying the RHD gene approximated 2.5% among serologically D-Tunisian individuals 7,15 and 25% among D-with C1 or E1 antigens.…”

Section: Conclusion: Among the Weak D Phenotypes Inmentioning

confidence: 99%

“…23 The molecular causes of the serologic D-, 7,9,15,16 weak D, 7,[10][11][12][13][15][16][17][18] and partial D phenotypes 7,10,[17][18][19] have been evaluated in different sets of Tunisian samples ranging from 100 to 2000 samples each. Similar to Caucasian populations, 24,25 the prevalence of individuals carrying the RHD gene approximated 2.5% among serologically D-Tunisian individuals 7,15 and 25% among D-with C1 or E1 antigens. 16 Likewise, the RHD gene deletion (RHD*01N.01) was most common (97.5%), while RHD*W (0.7%) and various RHD-CE-D hybrid alleles (1.1%) were rare, but some weak D types (0.7%), including two weak D Type 4.0 (0.2%), were also found.…”

Section: Conclusion: Among the Weak D Phenotypes Inmentioning

confidence: 99%

Transfusion strategy for weak D Type 4.0 based on RHD alleles and RH haplotypes in Tunisia

et al. 2017

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Background With more than 460 RHD alleles, this gene is the most complex and polymorphic among all blood group systems. The Tunisian population has the largest known prevalence of weak D type 4.0 alleles, occurring in 1 of 105 RH haplotypes. We aimed to establish a rationale for the transfusion strategy of weak D type 4.0 in Tunisia. Study design and methods Donors were randomly screened for the serological weak D phenotype. The RHD coding sequence and parts of the introns were sequenced. To establish the RH haplotype, the RHCE gene was tested for characteristic single nucleotide positions. Results We determined all RHD alleles and the RH haplotypes coding for the serologic weak D phenotype among 13,431 Tunisian donations. A serologic weak D phenotype was found in 67 individuals (0.50%). Among them, 60 carried a weak D type 4 allele: 53 weak D type 4.0, 6 weak D type 4.2.2 (DAR), and 1 weak D type 4.1. Another 4 donors had 1 variant allele each: DVII, weak D type 1, weak D type 3, and weak D type 100, while 3 donors showed a normal RHD sequence. The weak D type 4.0 was most often linked to RHCE*ceVS.04.01, weak D type 4.2.2 to RHCE*ceAR, and weak D type 4.1 to RHCE*ceVS.02, while the other RHD alleles were linked to one of the common RHCE alleles. Conclusions Among the weak D phenotypes in Tunisia, no novel RHD allele was found and almost 90% were caused by alleles of the weak D type 4 cluster, of which 88% represented the weak D type 4.0 allele. Based on established RH haplotypes for variant RHD and RHCE alleles and the lack of adverse clinical reports, we recommend D positive transfusions for patients with weak D type 4.0 in Tunisia.

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Transfusion management of Africans with RHD variants in China

Yin¹,

Ouchari

2023

Transfusion Clinique et Biologique

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RHD genotyping and its implication in transfusion practice

Cited by 7 publications

References 25 publications

RHD Genotyping by Molecular Analysis of Hybrid Rhesus box in RhD-Negative Blood Donors from Iran

RHD Genotyping by Molecular Analysis of Hybrid Rhesus box in RhD-Negative Blood Donors from Iran

Transfusion strategy for weak D Type 4.0 based on RHD alleles and RH haplotypes in Tunisia

Transfusion management of Africans with RHD variants in China

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