Rhein Inhibits the Progression of Chemoresistant Lung Cancer Cell Lines via the Stat3/Snail/MMP2/MMP9 Pathway

Liu, Jinxin; Ding, Derong; Liu, Feiye; Chen, Yizhi

doi:10.1155/2022/7184871

Cited by 13 publications

(14 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, we found that MMP2 was signi cantly up-regulated in SMV-treated HBECs and it was involved in many functions such as tissue repair, in ammation, vasculature remodeling, angiogenesis, and tumor invasion (Table 4). The nding was consistent with the previous reports [22][23][24]. MMP2 might be closely associated with the lung cancer cell proliferation and metastasis [22].…”

Section: Discussionsupporting

confidence: 93%

See 1 more Smart Citation

Gene Expression Responses in Bronchial Epithelial Cells Exposed to Vanadium

Abdel-Moneim

Yang

2022

Preprint

View full text Add to dashboard Cite

Vanadium exposure has the negative effect on lung health in human, whereas the detailed mechanisms of vanadium exposure-induced pulmonary toxicity are limited. Hence, the present study aimed to investigate the hub genes and signaling pathways related to sodium metavanadate (SMV)-induced pulmonary toxicity. The transcript expression profile GSE36684 was downloaded from Gene Expression Omnibus. The profile contained eight human bronchial epithelial cell (HBEC) samples including five SMV-treated and three control HBECs samples. Totally 455 differentially expressed genes (DEGs) were screened, especially 201 and 254 genes were up- and down-regulated in the HBECs treated with SMV. Gene ontology analysis suggested that the DEGs were mainly involved in signal transduction, the response to drug, cell proliferation, adhesion and migration. Pathway analysis demonstrated the DEGs were primarily participated in NF-κB, Wnt, MAPK, and PI3K-Akt signaling pathways. Moreover, the hub genes, including ITGA5, ITGB3, ITGA2, LAMC2, MMP2, and ITGA4, might contribute to SMV-induced pulmonary toxicity. Our study improves the understanding of the molecular mechanisms by which SMV induced the pulmonary toxicity.

show abstract

Section: Discussionsupporting

confidence: 93%

“…The nding was consistent with the previous reports [22][23][24]. MMP2 might be closely associated with the lung cancer cell proliferation and metastasis [22]. MMP2 also played a main regulatory role in the cytotoxicity induced by amyloid-beta in lung cancer cells [23].…”

Section: Discussionsupporting

confidence: 92%

Gene Expression Responses in Bronchial Epithelial Cells Exposed to Vanadium

Abdel-Moneim

Yang

2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Tumor metastasis is a complex process involving cell migration, invasion, and adhesion, and the ability of cell migration and invasion is usually detected using Transwell assays [ 51 ] ( Figure 9 C,D). MMP2 and MMP9 are metalloprotein kinases which degrade tissue barriers; furthermore, they are also related to epithelial–mesenchymal transition and are associated with cell migration and invasion [ 52 ]. At the cellular protein expression level, it can be found that the expression of MMP2 and MMP9, the key metalloprotein kinases associated with migration, was down-regulated in a concentration-dependent manner.…”

Section: Resultsmentioning

confidence: 99%

Isorhamnetin Induces Apoptosis and Suppresses Metastasis of Human Endometrial Carcinoma Ishikawa Cells via Endoplasmic Reticulum Stress Promotion and Matrix Metalloproteinase-2/9 Inhibition In Vitro and In Vivo

Zhang

et al. 2022

Foods

View full text Add to dashboard Cite

Endometrial cancer (EC) is a very common female cancer which has attracted more and more attention. According to the individual patient’s condition, the current treatment of EC patients is mainly based on surgery, which is supplemented by chemotherapy, radiotherapy, and endocrine intervention. However, these existing treatment strategies also have some inevitable limitations. Therefore, it is particularly important to find an active ingredient with low toxicity and a high safety profile against EC. Isorhamnetin is a flavonoid known to be present in a variety of plants, such as sea buckthorn, dry willow, and wolfberry. In recent years, the anti-tumor effects of isorhamnetin have been reported. In our study, isorhamnetin was shown to induce apoptosis in Ishikawa cells by inducing the endogenous mitochondrial apoptotic pathway and exogenous death receptor pathway, promoting the endoplasmic reticulum stress-related pathway, and activating the corresponding markers of UPR response. In addition, isorhamnetin affected the expression of MMP2 and MMP9-related proteins in vitro and in vivo and eventually repressed metastasis. Therefore, isorhamnetin can be used as a promising medicinal material for the treatment of EC.

show abstract

“…Yuan et al showed that 100 and 200 μM of rhein had cytotoxicity to colorectal cancer cells (Yuan et al, 2018). Recently, Liu et al used 25 and 50 μg/mL (i.e., 87.96 and 175.92 μM) of rhein to evaluate the anti‐proliferative and anti‐metastatic effects of rhein on the progression of chemoresistant lung cancer cells (Liu et al, 2022). Gao et al used 10, 20, 40, 80, and 160 μM of rhein to study the anticancer effect of rhein on gastric cancer cells (Gao et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of hypoxia‐induced HIF‐1α‐mediated autophagy enhances the in vitro antitumor activity of rhein in pancreatic cancer cells

Yao

Wang

2022

J of Applied Toxicology

View full text Add to dashboard Cite

A hypoxic microenvironment results in significantly elevated hypoxia-inducible factor-1 (HIF-1) level in pancreatic cancer. HIF-1 functions to maintain the survival of cancer cells. The present study was performed to investigate whether inhibition of HIF-1α expression was involved in the in vitro antitumor effect of rhein in pancreatic cancer cells and to explore the underlying mechanism. sh-RNA knockout technique and western blotting were used to investigate the role of HIF-1α in autophagy activation in MiaPaCa-2 and PANC-1 cells. The survival and glycolysis were assessed using MTT assay and colorimetric kits, respectively. Apoptosis was evaluated by detecting the levels of apoptosis-related proteins using western blotting. Among the five pancreatic cancer cell lines, MiaPaCa-2 and PANC-1 cells were more sensitive to hypoxia-induced autophagy. HIF-1α regulated hypoxia-induced autophagy in MiaPaCa-2 and PANC-1 cells. Treatment with rhein inhibited the survival and suppressed glycolysis in MiaPaCa-2 and PANC-1 cells exposed to hypoxia. Bafilomycin A1 enhanced the suppressive effects of rhein on cell survival and glycolysis under hypoxia. Treatment with rhein, but not bafilomycin A1, significantly reduced HIF-1α expression. In conclusion, inhibition of HIF-1α-mediated autophagy enhances the in vitro antitumor activity of rhein in pancreatic cancer cells under hypoxia.

show abstract

Rhein Inhibits the Progression of Chemoresistant Lung Cancer Cell Lines via the Stat3/Snail/MMP2/MMP9 Pathway

Cited by 13 publications

References 34 publications

Gene Expression Responses in Bronchial Epithelial Cells Exposed to Vanadium

Gene Expression Responses in Bronchial Epithelial Cells Exposed to Vanadium

Isorhamnetin Induces Apoptosis and Suppresses Metastasis of Human Endometrial Carcinoma Ishikawa Cells via Endoplasmic Reticulum Stress Promotion and Matrix Metalloproteinase-2/9 Inhibition In Vitro and In Vivo

Inhibition of hypoxia‐induced HIF‐1α‐mediated autophagy enhances the in vitro antitumor activity of rhein in pancreatic cancer cells

Contact Info

Product

Resources

About