Rheinheimera arenilitoris sp. nov., isolated from seashore sand

Park, Sooyeon; Park, Jimin; Won, Sung‐Min; Jung, Yong-Taek; Yoon, Jung‐Hoon

doi:10.1099/ijs.0.067504-0

“…Interestingly, Rheinheimera spp. are found in marine ( 75 ), coastal ( 76 – 78 ), freshwater ( 79 – 81 ), industrial waste ( 82 , 83 ), and alkaline environments ( 84 , 85 ). One strain produces calcite ( 86 )—potentially applicable for concrete biorepair—and another has recently been associated with stainless steel corrosion ( 87 ).…”

Section: Discussionmentioning

confidence: 99%

Bacterial Communities in Concrete Reflect Its Composite Nature and Change with Weathering

Keffer

¹

,

Maresca

²

2021

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Concrete is an extreme but common environment and is home to microbial communities adapted to alkaline, saline, and oligotrophic conditions. Microbes inside the concrete that makes up buildings or roads have received little attention despite their ubiquity and capacity to interact with the concrete. Because concrete is a composite of materials which have their own microbial communities, we hypothesized that the microbial communities of concrete reflect those of the concrete components and that these communities change as the concrete ages. Here, we used a 16S amplicon study to show how microbial communities change over 2 years of outdoor weathering in two sets of concrete cylinders, one prone to the concrete-degrading alkali-silica reaction (ASR) and the other having the risk of the ASR mitigated. After identifying and removing taxa that were likely laboratory or reagent contaminants, we found that precursor materials, particularly the large aggregate (gravel), were the probable source of ∼50 to 60% of the bacteria observed in the first cylinders from each series. Overall, community diversity decreased over 2 years, with temporarily increased diversity in warmer summer months. We found that most of the concrete microbiome was composed of Proteobacteria, Firmicutes, and Actinobacteria, although community composition changed seasonally and over multiyear time scales and was likely influenced by environmental deposition. Although the community composition between the two series was not significantly different overall, several taxa, including Arcobacter, Modestobacter, Salinicoccus, Rheinheimera, Lawsonella, and Bryobacter, appear to be associated with ASR. IMPORTANCE Concrete is the most-used building material in the world and a biologically extreme environment, with a microbiome composed of bacteria that likely come from concrete precursor materials, aerosols, and environmental deposition. These microbes, though seeded from a variety of materials, are all subject to desiccation, heating, starvation, high salinity, and very high pH. Microbes that survive and even thrive under these conditions can potentially either degrade concrete or contribute to its repair. Thus, understanding which microbes survive in concrete, under what conditions, and for how long has potential implications for biorepair of concrete. Further, methodological pipelines for analyzing concrete microbial communities can be applied to concrete from a variety of structures or with different types of damage to identify bioindicator species that can be used for structural health monitoring and service life prediction.

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“…[1] with the description of Rheinheimera baltica . At the time of writing, the genus Rheinheimera contained 25 validly published species (www.bacterio.net/genus/rheinheimera), which were reported from diverse habitats such as the Baltic Sea [1], the East Sea [2], deep seawater [3], marine sediment [4], coastal sediment [5, 6], seashore sand [7], tidal flat [8], saline lake [9], alkaline lake [10], freshwater [11], fishing hooks [12], soil [13] and rice roots [14]. Members of the genus Rheinheimera are Gram-stain-negative, rod- or cocci-shaped, aerobic or facultatively aerobic, and oxidase-positive, with Q-8 as the predominant respiratory quinone.…”

Section: Full-textmentioning

confidence: 99%

Rheinheimera mangrovi sp. nov., a bacterium isolated from mangrove sediment

Zhang

¹

,

Zhu

²

,

Cheng

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, rod-shaped, motile and strictly aerobic bacterium, designated LHK132T, was isolated from a mangrove sediment sample collected in Haikou, Hainan Province, PR China. Strain LHK132T was able to grow at temperatures of 10–45 °C, at salinities of 0–7.0 % (w/v) and at pH 6.0–9.0. Catalase and oxidase activities, H2S production, urease and methyl red reaction were positive. Indole, nitrate reduction, hydrolysis of gelatin, starch, casein and Tweens 20, 40, 60 and 80 were negative. The major cellular fatty acids were C16 : 0 and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c). The only respiratory quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. According to 16S rRNA gene sequence analysis, strain LHK132T had 98.3, 97.5, 97.4, 97.2 and 97.1% similarities to Rheinheimera soli BD-d46T, Rheinheimera sediminis YQF-1T, Rheinheimera tangshanensis JA3-B52T, Rheinheimera mesophila IITR-13T and Rheinheimera arenilitoris J-MS1T, respectively. Phylogenetic analyses indicated that strain LHK132T formed a distinct lineage with R. soli BD-d46T within the genus Rheinheimera . The average nucleotide identity and digital DNA–DNA hybridization values between strain LHK132T and related species of the genus Rheinheimera were well below the thresholds for species delineation. The DNA G+C content was 46.7 mol%. On the basis of its phenotypic, chemotaxonomic and genotypic data, strain LHK132T is considered a representative of a novel species in the genus Rheinheimera , for which the name Rheinheimera mangrovi sp. nov. is proposed. The type strain is LHK132T (=KCTC 62580T=MCCC 1K03529T).

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“…On 2 March 2021, the genus comprised 26 species with validly published names. Some species of this genus have been isolated from fishing hook [3], coastal sediment [4, 5], seawater [6], seashore sand and sediment [7], the central Baltic Sea [1], and deep-sea water [8]. Here, we report the taxonomic characteristics of a novel Rheinheimera species isolated from coastal sediment collcted in Jiangsu Province.…”

Section: Introductionmentioning

confidence: 99%

Rheinheimera lutimaris sp. nov., a marine bacterium isolated from coastal sediment

Yu

¹

,

Qi

²

,

Jing

³

2021

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain YQF-2T, was isolated from coastal sediment sampled in Jiangsu Province and characterized phylogenetically and phenotypically. Optimal bacterial growth occurred at 28 °C (range 4–38 °C) and pH 7 (pH 6–10). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YQF-2T was related to members of the genus Rheinheimera and shared the highest sequence identities with Rheinheimera pacifica KMM 1406T (98.6%), followed by Rheinheimera aestuarii H29T (98.4%), Rheinheimera japonica KMM 9513T (98.3%), Rheinheimera aquimaris SW-353T (98.3%), Rheinheimera hassiensis E48T (97.8%) and Rheinheimera muenzenbergensis E49T (97.7%). The 16S rRNA gene sequence identities between strain YQF-2T and other members of the genus Rheinheimera were below 97.2%. The digital DNA–DNA hybridization value between strain YQF-2T and R. pacifica KMM 1406T was 23.3±2.3%. The average nucleotide identity value between strain YQF-2T and R. pacifica KMM 1406T was 79.7%. The unique respiratory quinone was ubiquinone-8. Phosphatidylethanolamine and phosphatidylglycerol were identified as the major polar lipids. The strain had summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C16:0, C12:0 3-OH and iso-C17:0 3-OH as major fatty acids. The G+C content of the genomic DNA was 50.0 mol%. On the basis of phenotypic, genotypic and phylogenetic evidence, strain YQF-2T represents a novel species of the genus Rheinheimera , for which the name Rheinheimera lutimaris sp. nov. is proposed, with the type strain YQF-2T (=KCTC 72184T=MCCC 1K03663T).

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Rheinheimera arenilitoris sp. nov., isolated from seashore sand

Cited by 20 publications

References 34 publications

Bacterial Communities in Concrete Reflect Its Composite Nature and Change with Weathering

Bacterial Communities in Concrete Reflect Its Composite Nature and Change with Weathering

Rheinheimera mangrovi sp. nov., a bacterium isolated from mangrove sediment

Rheinheimera lutimaris sp. nov., a marine bacterium isolated from coastal sediment

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