2014
DOI: 10.1007/s00775-014-1226-2
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Rhenium and technetium tricarbonyl, {M(CO)3}+ (M = Tc, Re), binding to mammalian metallothioneins: new insights into chemical and radiopharmaceutical implications

Abstract: This paper deals with the binding of the four mammalian metallothioneins (MTs) to the organometallic metal fragment {fac-M(CO)3}(+) (M = (99)Tc, Re), which is highly promising for the preparation of second-generation radiopharmaceuticals. The study of the transmetallation reaction between zinc and rhenium in Zn7-MT1 by means of UV-vis and CD spectroscopy demonstrated the incorporation of the {fac-Re(CO)3}(+) fragment to the MTs. This reaction should be performed at 70 °C to accelerate the reaction rate, a resu… Show more

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Cited by 18 publications
(8 citation statements)
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“…It is also clear that this is much less of a problem during Zn(II) metalation because of the decreased pH sensitivity at physiological pH. MT also binds a number of exogenous heavy metals involved in chemotherapeutics [81][82][83] and water contamination 84 and the pH considerations raised in this paper likely apply to other MT-metal binding systems. Similar metalation experiments with human MT2a show a mixed mechanism at pH 7.4 30 and rabbit liver MT2a has been shown by NMR to cluster at pH 7.2, with the signal disappearing at higher pH.…”
Section: Understanding Mt1a's Multitude Of Biological Functionsmentioning
confidence: 86%
“…It is also clear that this is much less of a problem during Zn(II) metalation because of the decreased pH sensitivity at physiological pH. MT also binds a number of exogenous heavy metals involved in chemotherapeutics [81][82][83] and water contamination 84 and the pH considerations raised in this paper likely apply to other MT-metal binding systems. Similar metalation experiments with human MT2a show a mixed mechanism at pH 7.4 30 and rabbit liver MT2a has been shown by NMR to cluster at pH 7.2, with the signal disappearing at higher pH.…”
Section: Understanding Mt1a's Multitude Of Biological Functionsmentioning
confidence: 86%
“…The lack of a rigid structure allows MTs to coordinate a number of metals including essential metals like zinc and copper [11], toxic metals like cadmium [12] and mercury [13], and exotic metals like uranium [14] and technetium [15]. This flexibility, while fundamental to their function, makes MTs extremely difficult to characterize structurally.…”
Section: Metallothioneinmentioning
confidence: 99%
“…As a consequence, a large number of protein-specific Re(CO) 3 complexes have been developed as cold analogs of the hot Tc complexes, with the intent to replace Re by its 99m Tc counterpart for the final application. Various proteins have been targeted, including Translocator Protein TSPO [82], metallothioneins [83], G Protein-Coupled Estrogen Receptor GPER/GPR30 [84] or Carbonic Anhydrase IX [85]. As an example, the synthesis of a series of M(CO) 3 -based (M = Re, Tc) Carbonic Anhydrase IX inhibitors was developed using Re(CO) 3 , while the affinity for CA-IX was evaluated using the final 99m Tc complexes [77].…”
Section: Re(co) 3 As Surrogates For 99mmentioning
confidence: 99%