Rheological Properties of a Two Phase Lipid Monolayer at the Air/Water Interface: Effect of the Composition of the Mixture

Wilke, Natalia; Mercado, Franco Vega; Maggio, Bruno

doi:10.1021/la100552j

Cited by 47 publications

(88 citation statements)

References 41 publications

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“…agreement with the fact that the studied monolayers are located in the regime of the condensed area fraction where the lever rule is valid 5 , and hence no φ -dependence of σ is expected. Furthermore, the dipolar repulsion obtained is of the same order of magnitude as that obtained with the parallel plate capacitor model.…”

Section: Discussionsupporting

confidence: 86%

“…At room temperature, the mixed monolayers show LC micrometer sized domains dispersed in a LE continuous phase, at a lateral pressure of 10 mN/m and for composition of DSPC higher than 24 mol% 5 .…”

Section: Methodsmentioning

confidence: 99%

“…When the denser phase is not the continuous phase, domains (named rafts for certain lipid composition) are observed, moving in the more fluid phase. The domains interact with each other [1][2][3][4] , and these interactions affect their own movement 4,5 as well as that of other species present in the membrane 6,7 .…”

Section: Introductionmentioning

confidence: 99%

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Dipolar interactions between domains in lipid monolayers at the air–water interface

2016

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A great variety of biologically relevant monolayers present phase coexistence characterized by domains formed by lipids in an ordered phase state dispersed in a continuous, disordered phase. The difference in surface densities between these phases originates inter-domain dipolar interactions, which are relevant for the determination of the spacial distribution of domains, as well as their dynamics. In this work, we propose a novel manner of estimating the dipolar repulsion using a passive method that involves the analysis of images of the monolayer with phase coexistence. The method is based on the comparison of the pair correlation function obtained from experiments with that obtained from Brownian dynamics simulations of a model system. As an example, we determined the difference in dipolar density of a binary monolayer of DSPC/DMPC at the air-water interface from the analysis of the radial distribution of domains, and the results are compared with those obtained by surface potential determinations. A systematic analysis for experimentally relevant parameter range is given, which may be used as a working curve for obtaining the dipolar repulsion in different systems.

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Section: Discussionsupporting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

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Dipolar interactions between domains in lipid monolayers at the air–water interface

2016

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“…[46]. The studies reported in the literature, involving DMPC, CHOL and DMPC:CHOL (7:3) mixture show slight differences that can be assigned to different experimental conditions such as buffer solution and temperature [47][48][49][50] The results obtained in this work remain within the dispersion found for the reported studies.…”

Section: Dsc Analysissupporting

confidence: 84%

Effect of tetracaine on DMPC and DMPC+cholesterol biomembrane models: Liposomes and monolayers

Serro

Galante

Kozica

et al. 2014

Colloids and Surfaces B: Biointerfaces

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Different types of lipid bilayers/monolayers have been used to simulate the cellular membranes in the investigation of the interactions between drugs and cells. However, to our knowledge, very few studies focused on the influence of the chosen membrane model upon the obtained results. The main objective of this work is to understand how do the nature and immobilization state of the biomembrane models influence the effect of the local anaesthetic tetracaine (TTC) upon the lipid membranes. The interaction of TTC with different biomembrane models of dimyristoylphosphatidylcholine (DMPC) with and without cholesterol (CHOL) was investigated through several techniques. A quartz crystal microbalance with dissipation (QCM-D) was used to study the effect on immobilized liposomes, while phosphorus nuclear magnetic resonance ( 31 P-NMR) and differential scanning calorimetry (DSC) were applied to liposomes in suspension. The effect of TTC on Langmuir monolayers of lipids was also investigated through surface pressure-area measurements at the air-water interface. The general conclusion was that TTC has a fluidizing effect on the lipid membranes and, above certain concentrations, induced membrane swelling or even solubilization. However, different models led to variable responses to the TTC action. The intensity of the disordering effect caused by TTC increased in the following order: supported liposomes < liposomes in solution < Langmuir monolayers. This means that extrapolation of the results obtain in in vitro studies of the lipid/anaesthetic interactions to in vivo conditions should be done carefully.

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“…In this manner, the elastic properties of the film do not change under the incorporation of a more solid state-prone lipid such as 14:0 Cer and, as long as the LE phase is able to accommodate the molecules, no phase separation occurs. The condensed domains in equilibrium with the expanded phase impose dynamic rigidity or stiffening upon compression but retain a global state typical of expanded phases (Wilke and Maggio 2009;Wilke et al 2010). In this manner, the increased amount of domains in the 18:0/10:0 Cer as compared to the 14:0/10:0 Cer mixture can also explain the higher values of the compressibility modulus by imposing more resistance to compression.…”

Section: Ceramide Mixturesmentioning

confidence: 99%

The many faces (and phases) of ceramide and sphingomyelin II – binary mixtures

Fanani

Maggio

2017

Biophys Rev

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A rather widespread idea on the functional importance of sphingolipids in cell membranes refers to the occurrence of ordered domains enriched in sphingomyelin and ceramide that are largely assumed to exist irrespective of the type of N-acyl chain in the sphingolipid. Ceramides and sphingomyelins are the simplest kind of two-chained sphingolipids and show a variety of species, depending on the fatty acyl chain length, hydroxylation, and unsaturation. Abundant evidences have shown that variations of the N-acyl chain length in ceramides and sphingomyelins markedly affect their phase state, interfacial elasticity, surface topography, electrostatics, and miscibility, and that even the usually conceived Bcondensed^sphingolipids and many of their mixtures may exhibit liquid-like expanded states. Their lateral miscibility properties are subtlety regulated by those chemical differences. Even between ceramides with different acyl chain length, their partial miscibility is responsible for a rich twodimensional structural variety that impacts on the membrane properties at the mesoscale level. In this review, we will discuss the miscibility properties of ceramide, sphingomyelin, and glycosphingolipids that differ in their N-acyl or oligosaccharide chains. This work is a second part that accompanies a previous overview of the properties of membranes formed by pure ceramides or sphingomyelins, which is also included in this Special Issue.

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Rheological Properties of a Two Phase Lipid Monolayer at the Air/Water Interface: Effect of the Composition of the Mixture

Cited by 47 publications

References 41 publications

Dipolar interactions between domains in lipid monolayers at the air–water interface

Dipolar interactions between domains in lipid monolayers at the air–water interface

Effect of tetracaine on DMPC and DMPC+cholesterol biomembrane models: Liposomes and monolayers

The many faces (and phases) of ceramide and sphingomyelin II – binary mixtures

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