Rheological Study of Lysozyme in Dimethyl Sulfoxide + Water Solution at 298.15 K

Kamiyama, Tadashi; Morita, Masatsugu; Kimura, Takayoshi

doi:10.1021/je049928n

Cited by 25 publications

(14 citation statements)

References 9 publications

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“…The corresponding hydrodynamic diameter [24] is 4.1 nm which is similar to the molecular size calculated by the molecular modeling of the monomeric 1 (Figure 5 b). Dynamic light scattering (DLS) clearly showed the aggregation of 1 under the same condition.…”

supporting

confidence: 79%

Self‐Assembled Nanostructures of Tailored Multi‐Metal Complexes and Morphology Control by Counter‐Anion Exchange

Yamamura

Sasaki

Kyotani

et al. 2010

Chemistry A European J

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Self-assembly is one of the most important and useful techniques for the convenient construction of nanostructures such as particles, fibers, layers, [1] which show a variety of attractive functional molecules and materials for use as a catalyst, sensor, material transporter, and optical material.[2] In most cases, the size and morphology of the nanostructure depend on the components, but the prediction of the overall structure of the nanostructure remains a difficult challenge. The supramolecular nanostructure based on dynamic and directed interactions between the components, such as hydrogen bonding, p-stacking, and coordination bonding with metal ions [3] has been widely used for the formation of shape-defined aggregations, such as wire [3] and tube [4] structures. Such a strategy showed great success for materials composed of a d-block metal complex known as metal-organic frameworks (MOFs).[5] Conversely, the number of reports on an aggregation composed of the f-block metal complex has been recently increasing because the assembly of lanthanide ions shows unique functions such as magnetic [6,7] and luminescent applications. [7] We reported the synthesis of the "trisaloph" ligand, a macrocyclic multidentate ligand composed of three saloph moieties, and its lanthanide-zinc heterotetranuclear complex.[8] Saloph ligands form planar complexes with a variety of metals, [9] which have been developed for supramolecular systems, such as anion-templated assemblies, [10] self-assembled fibers, [11] and liquid crystals.[12] The trisaloph ligand was also utilized as a module for a one-dimensional multi-metal supramolecule.[13] Alkali metal ions caused a stacked tubular aggregation of the trisaloph ligands due to p-p stacking and coordination to the metal ion.[13] The trisaloph lanthanide complex is expected to form a self-assembled aggregation due to the strong interaction between the metal ion and the counter anion of the cationic lanthanide complex that can act as a spacer ligand bridging the lanthanide because the rigid O6 site of the trisaloph fixes the vacant coordination sites of the lanthanide ion in a one-dimensional direction, that is, tailored to a fibril morphology. Herein, the unique aggregation of the trisaloph Zn II 3 La III complex bearing PEGylated adamantane units is successfully formed in an aqueous solution by complexation with b-cyclodextrin (b-CD). Additionally, the morphology of the nanostructure is controlled by coordinated anions based on the structural change in the trisaloph complex.All trisaloph ligands and their complexes reported thus far are insoluble in aqueous media. However, we designed and synthesized a trisaloph ligand bearing adamantane units as an appropriate candidate for water-soluble trisaloph metal complexes by taking advantage of the association of the adamantane units with b-CD.[14] The trisaloph complexes 1 (Figure 1) would be quite versatile because they should be soluble in organic solvents in the absence of b-CD due to high hydrophobicity of the adamantane units. Both aqueou...

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supporting

confidence: 79%

Self‐Assembled Nanostructures of Tailored Multi‐Metal Complexes and Morphology Control by Counter‐Anion Exchange

Yamamura

Sasaki

Kyotani

et al. 2010

Chemistry A European J

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“…Theoretical (Zhou, 1995) and experimental (Ahmad & Salahuddin, 1974;Lefebvre 1982;Areas et al, 1996;Monkos, 1997;Kamiyama et al, 2004;Pan et al, 2009) studies on the viscosity of various lysozyme solutions have been reported previously. According to a theoretical analysis (Zhou, 1995) the intrinsic viscosity is strongly influenced by the hydration level of the protein.…”

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confidence: 95%

Denaturation and aggregation of lysozyme in water-ethanol solution.

Szymańska

Hornowski²,

Ślósarek³

2012

Acta Biochim Pol

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We have applied rheological methods for the analysis of ethanol-lysozyme interaction during the process of denaturation and aggregation of the protein. At low concentration of ethanol a destruction of the hydration shell of lysozyme is observed. With the increase in the ethanol concentration a structural transformation takes place. It leads to the formation of a protein aggregate with an elongated structure. The rheological characteristics of lysozyme-water-ethanol solution changes from Newtonian to pseudoplastic.

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“…A high increase in the protein viscosity was also induced by other organic solvents like tetramethylurea (TMU) [29] and dimethylsulfoxide (DMSO) [29,30]. The pseudoplastic behavior of the protein in TMU indicated that the lysozyme existed in the elongated conformation and was able to reorient under shear stress.…”

Section: Resultsmentioning

confidence: 99%

The SAXS and Rheological Studies of HEWL Amyloid Formation

et al. 2008

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We performed small angle X-ray scattering and rheological experiments in order to analyze the aggregation and denaturation processes of hen egg white lysozyme initiated by the presence of ethanol molecule. At low ethanol concentrations (below 60% (v/v)) we did not observe any change of the radius of gyration of lysozyme and no drastic changes in viscosity of the protein solution. With the increase in ethanol concentration up to the final concentration of 85% (v/v) the viscosity of protein solution dramatically increased. For high ethanol concentration a pseudoplastic behavior of lysozyme solution was observed, indicating a process of aggregation and reorientation of the protein molecules. Similar effects were observed in small angle X-ray scattering experiments. We assume that the analysis of the aggregation processes of the hen egg white lysozyme could contribute to our understanding of the mechanism of lysozyme amyloid formation.

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Rheological Study of Lysozyme in Dimethyl Sulfoxide + Water Solution at 298.15 K

Cited by 25 publications

References 9 publications

Self‐Assembled Nanostructures of Tailored Multi‐Metal Complexes and Morphology Control by Counter‐Anion Exchange

Self‐Assembled Nanostructures of Tailored Multi‐Metal Complexes and Morphology Control by Counter‐Anion Exchange

Denaturation and aggregation of lysozyme in water-ethanol solution.

The SAXS and Rheological Studies of HEWL Amyloid Formation

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