Rheology and microstructural studies of regenerated silk fibroin solutions

Rheology of interfacial layers

“…It was assumed that the gel-like layer was formed by ordered ß-sheet structures. A similar behavior was reported by Nisal et al [48] for regenerated silk fibroin solutions.…”

In vitro study of novel microparticle based silk fibroin scaffold with osteoblast-like cells for load-bearing osteo-regenerative applications

“…It allows easy chemical modication 17 that is required to obtain required functionality and can also be processed into 3D materials such as hydrogels, sponges, bers, microspheres, and electrospun ber mats. 1,[18][19][20][21][22] The unique mechanical properties and tunable bioresorption rate of these 3D scaffolds allows attachment, proliferation and differentiation of mesenchymal stem cells along the osteogenic lineage for BTE. The efficacy of these 3D scaffolds from SF gets enhanced when they are surface functionalized with integrin binding RGD motifs, collagen, chitosan, gelatin and growth factors like transforming growth factor-b (TGF-b), VEG-F and bone morphogenic protein (BMPs).…”

Mixing and Flow-Induced Silk Fibroin Self-Assembly in Microfluidic and Semi-Batch Nanoprecipitation

“…Each experiment was repeated in triplicate using three different silk precursor stocks. Calculations for needle residence time and shear rate were based on the literature value for dynamic viscosity (27 mPas) of regenerated 3% aqueous silk 42 and the calculated density (1.02 g mL -1 ) for the 3% w/v aqueous silk solution, with an assumed Newtonian flow (Fig. 1b).…”

“…1b). 42 The Reynolds number was estimated as 2, based on the internal diameter of the needle 43 (330 μm) and indicated laminar flow. An upper limit of the residence time was estimated using the linear velocity (1.94 mm s -1 ) and the needle length.…”

“…50 Calculations were based on the literature viscosity value (3.14 mPas) and density (0.837 g mL - 1) values for the 5:1 v/v isopropanol/water mixture measured at 20 °C, 51 and Newtonian flow was assumed. 42 The mixing time was estimated at 1-21 ms, according to the manufacturer's guidelines and based on an analytical model for a similar system published elsewhere, by calculating the Peclet number to achieve a coefficient of variation of < 0.1 (Fig. 1c).…”