Rheotaxis quality index: a new parameter that reveals male mammalian <i>in vivo</i> fertility and low sperm DNA fragmentation

Yaghoobi, Mohammad Ali; Azizi, Mohammad; Mokhtare, Amir; Javi, Farhad; Abbaspourrad, Alireza

doi:10.1039/d2lc00150k

Cited by 12 publications

(14 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To quantify the sperm accumulation in the strictures, we have used the algorithm we developed for RHEOLEX. 41 This algorithm calculates the changes in pixel intensity for consecutive frames and obtains the average intensity for 200 images to generate signal contours (Fig. 3A).…”

Section: Resultsmentioning

confidence: 99%

“…Previously we developed a method of characterizing mammalian sperm rheotaxis and concluded that spermatozoa from bulls with higher fertilization rates show higher rheotaxis ability. 41 Here, we postulated that sorting spermatozoa with higher rheotaxis capability could result in better fertilization outcomes in IVF cycles. To test this, we designed and characterized a high throughput microfluidic platform to separate spermatozoa with various rheotaxis abilities within a network of parallel strictures.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we introduced the rheotaxis quality index (RHEOLEX) as a potential biomarker for fertility screening: the higher the number of spermatozoa with higher rheotaxis capacity, the higher the pregnancy outcomes. 41 We have now designed a microfluidic channel to separate motile spermatozoa based on their rheotaxis capability and then how spermatozoa selected at different flow rates impacts early embryonic development. We confirmed that the kinematic features of the separated spermatozoa (speed, beating amplitude and frequency) were tuned by the intensity of the flow rate.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Faster sperm selected by rheotaxis leads to superior early embryonic development in vitro

Yaghoobi,

Abdelhady,

Favakeh

et al. 2024

Lab Chip

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Faster sperm selected by rheotaxis leads to superior early embryonic development in vitro

Yaghoobi,

Abdelhady,

Favakeh

et al. 2024

Lab Chip

Self Cite

View full text Add to dashboard Cite

show abstract

“…Human sperm from samples showing greater positive rheotaxis were demonstrated to have more normal morphology and better genomic quality ( De Martin et al, 2017 ; Ataei et al, 2021 ; Sharma et al, 2022 ). Recently, it was reported that rheotaxis success of bull sperm was positively correlated with bull fertility ( Yaghoobi et al, 2022 ). Similarly, it has been observed that the selection of rheotactic Mus musculus mouse sperm in vitro increased fertilization success and quality of early embryonic development ( Romero-Aguirregomezcorta et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Biological benefits of collective swimming of sperm in a viscoelastic fluid

Phuyal

Suárez

Tung

2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Collective swimming is evident in the sperm of several mammalian species. In bull (Bos taurus) sperm, high viscoelasticity of the surrounding fluid induces the sperm to form dynamic clusters. Sperm within the clusters swim closely together and align in the same direction, yet the clusters are dynamic because individual sperm swim into and out of them over time. As the fluid in part of the mammalian female reproductive tract contains mucus and, consequently, is highly viscoelastic, this mechanistic clustering likely happens in vivo. Nevertheless, it has been unclear whether clustering could provide any biological benefit. Here, using a microfluidic in vitro model with viscoelastic fluid, we found that the collective swimming of bull sperm in dynamic clusters provides specific biological benefits. In static viscoelastic fluid, clustering allowed sperm to swim in a more progressive manner. When the fluid was made to flow in the range of 2.43–4.05 1/sec shear rate, clustering enhanced the ability of sperm to swim upstream. We also found that the swimming characteristics of sperm in our viscoelastic fluid could not be fully explained by the hydrodynamic model that has been developed for sperm swimming in a low-viscosity, Newtonian fluid. Overall, we found that clustered sperm swam more oriented with each other in the absence of flow, were able to swim upstream under intermediate flows, and better withstood a strong flow than individual sperm. Our results indicate that the clustering of sperm can be beneficial to sperm migrating against an opposing flow of viscoelastic fluid within the female reproductive tract.

show abstract

“…Motilitas spermatozoa merupakan parameter yang sangat penting untuk mengevaluasi tingkat fertilitas spermatozoa (Yaghoobi et al, 2022). Nilai rata-rata persentase motilitas berdasarkan hasil uji Duncan (α=0,05) menunjukkan adanya perbedaan nyata pada setiap perlakuan (Tabel 1)…”

Section: Motilitas Spermatozoaunclassified

The Effect of Sugarcane Water Subtitution as a Candidate Substitute for Fructose in CEP Diluent on Spermatozoa Quality of Friesian Holstein Bull during Frozen Storage

Rahayu

Ducha²

2022

JIPT

View full text Add to dashboard Cite

Sugarcane water contains sucrose which function as an energy source to increase ATP and motility of sprematozoa. The purpose of this study was to determine the effect of sugarcane water substitution as a candidate for fructose substitute in CEP diluent on the sperm quality of Friesian Holstein (FH) bull in frozen storage. The type of research used was experimental research with Completely Randomized Design (CRD) with 5 treatments and 4 repetitions, namely A (CEP); B (CEP without fructose + 15% sugarcane water); C (CEP without fructose + 20% sugarcane water); D (CEP without fructose + 25% sugarcane water); E (CEP without fructose + 30% sugarcane water). Parameters used were motility, viability, and membrane integrity of FH bull spermatozoa. The motility test was observed with a 400x magnification microscope. The viability test was carried out with eosin-negrosin dye and observed with a 400x magnification microscope. The membrane integrity test was carried out with HOST fluid and observed with a 400x magnification microscope. Data analysis used the Kolmogorov-Smirnov test, then the One-Way ANOVA test and Duncan’s test. The result showed that the treatment with CEP diluent had the best results in maintaining the quality of spermatozoa during frozen storage with an average motility, viability and membrane integrity of 40,11±0,41%; 46,71±0,15% and 45,88±0,77% respectively.

show abstract

Rheotaxis quality index: a new parameter that reveals male mammalian in vivo fertility and low sperm DNA fragmentation

Abstract: The female reproductive tract simultaneously guides and selects high-quality sperm using rheotaxis in mammalian species. Sperm quality, however, is traditionally evaluated only by their movement velocities and concentration using computer-assisted...

Cited by 12 publications

References 48 publications

Faster sperm selected by rheotaxis leads to superior early embryonic development in vitro

Faster sperm selected by rheotaxis leads to superior early embryonic development in vitro

Biological benefits of collective swimming of sperm in a viscoelastic fluid

The Effect of Sugarcane Water Subtitution as a Candidate Substitute for Fructose in CEP Diluent on Spermatozoa Quality of Friesian Holstein Bull during Frozen Storage

Contact Info

Product

Resources

About