rhG-CSF does not affect the phenotype of adult donor peripheral blood NK cells

Abstract

Summary:Considerable evidence in preclinical models as well as in human transplantation now suggests that donor-derived natural killer (NK) cells can contribute to alloimmune recognition of recipient residual tumour cells. This makes the NK cell population an attractive target for in vitro or in vivo manipulations, in order to improve the antitumour effect of allogeneic transplantation. However, conditions in which allogeneic donor cells are collected vary; several reports have emphasised the different phenotypic and functional properties of T cells derived from marrow, cord blood or mobilised peripheral blood grafts; others have demonstrated different clinical outcomes following blood or marrow transplantation after myeloablative conditioning regimens. NK cells have been examined in this setting; the availability of new tools to study the expression of a variety of surface antigens that are involved in the control of NK cell activity offered us an opportunity to extensively characterise the phenotypic properties of NK cells from donors, before and after administration of pharmacological doses of rhG-CSF used for haematopoietic progenitor mobilisation. Our study suggests that rhG-CSF does not reproducibly alter blood NK cell phenotype in normal individuals, and thus that donor-derived cells are fully equipped to exert their potential antitumour effect. Bone Marrow Transplantation (2005) 35, 25-32.

Hematopoietic, Mesenchymal, and Immune Cells Are More Enhanced in Bone Marrow than in Peripheral Blood from Granulocyte Colony-Stimulating Factor Primed Healthy Donors

“…In contrast with previously reported data showing no significant effect of G-CSF on NK subpopulations [23,39], our results show that although in PB the NK cell fractions were significantly down-regulated by G-CSF, in BM a modest increase of both CD3 − CD16 + and CD3 − CD56 + was detected. This activity of G-CSF on BM NK cells might be of particular importance in G-BM haploidentical transplant because NK cells are important effector cells in mediating the graft-versusleukemia effect.…”

Effects and safety of granulocyte colony-stimulating factor in healthy volunteers

“…One study [34] showed no effect of rhG-CSF administration on the PB NK cell phenotype (defined as CD3 - /CD56/CD16 + or CD3 - /CD56 + cells) of adult PBPC donors.…”

Hematopoietic growth factors—use in normal blood and stem cell donors: clinical and ethical issues

“…G‐CSF administration typically leads to a two‐ to threefold increase in the number of eosinophils and enhanced eosinophil adhesion 11,12 . Existing data indicate minimal or no impact of G‐CSF on natural killer cells 13 . The neutrophil kinetics after G‐CSF administration have been well characterized for many years.…”

“…11,12 Existing data indicate minimal or no impact of G-CSF on natural killer cells. 13 The neutrophil kinetics after G-CSF administration have been well characterized for many years. G-CSF administration increases polymorphonuclear (PMN) leukocyte production, expands the promyelocye-myelocyte compartment of the marrow (however, the percentage of marrow blasts remains unchanged), and reduces PMN leukocyte tissue migration.…”