Rhizobia of chickpea from southern Portugal: symbiotic efficiency and genetic diversity

Laranjo, Marta; Rodrigues, R.; Alho, L.; Oliveira, Solange

doi:10.1046/j.1365-2672.2001.01295.x

Cited by 23 publications

(17 citation statements)

References 20 publications

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“…The present study indicates the existence of different levels of heterogeneity within natural chickpea rhizobium populations, confirming previous studies [3,4,12,13] and refuting the original idea that chickpea-infective strains should be a very homogeneous group [5,32]. The results obtained with 16S-rDNA sequencing, DAPD analysis and whole-cell protein fingerprinting provide further evidence of higher genetic diversity within the population of Beja, as previously suggested by plasmid profile analysis [12,13]. Beja isolates are distributed in clusters A, C and D. On the other hand, Evora population seems to be the most homogeneous, since most isolates are grouped within cluster A.…”

Section: Ecological Aspectssupporting

confidence: 92%

High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region

Laranjo

Machado

Young

et al. 2004

FEMS Microbiology Ecology

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Chickpea rhizobia isolated from Portuguese soils were assigned to the genus Mesorhizobium by 16S-rDNA sequencing. High species diversity was found within populations of an agricultural region in the south of Portugal. Besides the expected Mesorhizobium ciceri and M. mediterraneum, some isolates were close to M. loti or M. tianshanense and some formed a clade that may represent a new species. A new PCR-based approach, named direct amplified polymorphic DNA (DAPD) analysis, supported the 16S-based phylogeny. This suggests that this method could be used as a molecular tool to assess genetic relationships. Evaluation of genetic diversity by 16S-rDNA sequence, DAPD and protein profiles showed different levels of heterogeneity in natural populations.

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Section: Ecological Aspectssupporting

confidence: 92%

High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region

Laranjo

Machado

Young

et al. 2004

FEMS Microbiology Ecology

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“…Five isolates were selected for further study: C-1-Coimbra, C-27b-Coimbra, V-15b-Viseu, V-18-Viseu and V-20-Viseu. Four additional isolates obtained previously and identified by 16S rRNA gene analysis (Laranjo et al, 2001(Laranjo et al, , 2002(Laranjo et al, , 2004 were used.…”

Section: Methodsmentioning

confidence: 99%

dnaJ is a useful phylogenetic marker for alphaproteobacteria

Alexandre

Laranjo

Young

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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In the past, bacterial phylogeny relied almost exclusively on 16S rRNA gene sequence analysis. More recently, multilocus sequence analysis has been used to infer organismal phylogenies. In this study, the dnaJ chaperone gene was investigated as a marker for phylogeny studies in alphaproteobacteria. Preliminary analysis of G+C contents and G+C3s contents (the G+C content of the synonymous third codon position) showed no clear evidence of horizontal transfer of this gene in proteobacteria. dnaJ-based phylogenies were then analysed at three taxonomic levels: the Proteobacteria, the Alphaproteobacteria and the genus Mesorhizobium. Dendrograms based on DnaJ and 16S rRNA gene sequences revealed the same topology described previously for the Proteobacteria. These results indicate that the DnaJ phylogenetic signal is able to reproduce the accepted relationships among the five classes of the Proteobacteria. At a lower taxonomic level, using 20 alphaproteobacteria, the 16S rRNA gene-based phylogeny is distinct from the one based on DnaJ sequence analysis. Although the same clusters are generated, only the topology of the DnaJ tree is consistent with broader phylogenies from recent studies based on concatenated alignments of multiple core genes. For example, the DnaJ tree shows the two clusters within the Rhizobiales as closely related, as expected, while the 16S rRNA gene-based phylogeny shows them as distantly related. In order to evaluate the phylogenetic performance of dnaJ at the genus level, a multilocus analysis based on five housekeeping genes (atpD, gapA, gyrB, recA and rplB) was performed for ten Mesorhizobium species. In contrast to the 16S rRNA gene, the DnaJ sequence analysis generated a tree similar to the multilocus dendrogram. For identification of chickpea mesorhizobium isolates, a dnaJ nucleotide sequence-based tree was used. Despite different topologies, 16S rRNA gene-and dnaJ-based trees led to the same species identification. This study suggests that the dnaJ gene is a good phylogenetic marker, particularly for the class Alphaproteobacteria, since its phylogeny is consistent with phylogenies based on multilocus approaches. INTRODUCTIONThe phylum Proteobacteria is composed of five classes, the Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Deltaproteobacteria and Epsilonproteobacteria (Garrity et al., 2005;Stackebrandt et al., 1988). By the end of 2008, more than 380 complete genomes of proteobacteria were available in public databases (http://www. ncbi.nlm.nih.gov/genomes/lproks.cgi). Alphaproteobacteria exhibit an enormous diversity in their morphological and metabolic characteristics and the class is presently recognized solely as a clade in the 16S rRNA gene-based phylogeny (Stackebrandt et al., 1988). Based on 16S rRNA gene trees, the Alphaproteobacteria has been divided into seven orders: Caulobacterales, Rhizobiales, Rhodobacterales, Rhodospirillales, Rickettsiales, Sphingomonadales and Parvularculales (Kersters et al., 2006). The Alphaproteobacteria includes important bacteria tha...

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“…The symbiosis between chickpea and its specific rhizobia has been recently studied in several countries-Tunisia (Aouani et al 2001a), Morocco (Maatallah et al 2002), Canada (Kyei-Boahen et al 2002), Turkey (Içgen et al 2002) and Portugal (Laranjo et al 2001(Laranjo et al , 2002)-because of the promising agricultural usefulness of this crop as a grain legume for human and animal nutrition and because of the interesting extreme host specificity of its rhizobia. In Tunisian soils, chickpea is poorly nodulated (Aouani et al 2001a) and seeds must be inoculated to enhance productivity of the plant.…”

Section: Introductionmentioning

confidence: 99%

Competition for nodule formation between introduced strains of Mesorhizobium ciceri and the native populations of rhizobia nodulating chickpea (Cicer arietinum) in Tunisia

Romdhane

Tajini

Trabelsi

et al. 2007

World J Microbiol Biotechnol

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Chickpea is the most cultivated grain legume in the world and it shares the first rank with faba bean in Tunisia. However, the yield remains low, mainly due to the limited availability of N and P, and to the severe bioclimatic conditions. No inoculation trials had been conducted on chickpea in the Tunisian soils. This paper reports the yield response to inoculation by two different strains of Mesorhizobium ciceri, an exogenous type strain (UPMCa7 T ) and a selected local strain (CMG6). Field experiments were conducted in different sites in the north of Tunisia using three chickpea cultivars (cvs. Amdoun I, Chetoui and Kasseb). Rhizobia occupying field nodules were isolated and identified using 16S rDNA typing for both inoculated and non-inoculated plots. In contrast to the exogenous strain, the local strain gave a significant increase in nodule number and shoot dry yield in all the experimental fields for the three cultivars used. Monitoring of the nodule occupancy showed that the local strain competed well the native populations of rhizobia. The usefulness and the persistence of this strain in the different soils where it was introduced will be assessed further during the next years.

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Rhizobia of chickpea from southern Portugal: symbiotic efficiency and genetic diversity

Cited by 23 publications

References 20 publications

High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region

High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region

dnaJ is a useful phylogenetic marker for alphaproteobacteria

Competition for nodule formation between introduced strains of Mesorhizobium ciceri and the native populations of rhizobia nodulating chickpea (Cicer arietinum) in Tunisia

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