Rhizobium Impacts on Seed Productivity, Quality, and Protection of Pisum sativum upon Disease Stress Caused by Didymella pinodes: Phenotypic, Proteomic, and Metabolomic Traits

Sistani, Nima Ranjbar; Kaul, Hans‐Peter; Desalegn, Getinet; Wienkoop, Stefanie

doi:10.3389/fpls.2017.01961

Cited by 50 publications

(45 citation statements)

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“…In line with processes carried out before (Ranjbar Sistani et al, 2017) and according to Begum et al (2001), the disinfected pea seeds (ethanol 95%) were washed by ultrapure water and then, were kept (20 min) in bleach 5% and rinsed by ultrapure water several times. Finally, the soaked seeds (4 h) were considered for planting (Begum et al, 2001).…”

Section: Performing Experiments and Plantingmentioning

confidence: 99%

“…According to our previous study (Ranjbar Sistani et al, 2017) and in detail: the prepared pathogen (D. pinodes) by Rubiales Lab in Spain was multiplied on Potato Dextrose Agar (PDA) and incubated upon 12-h photoperiod and 22°C (Davidson et al, 2012). Incubated colonies of pathogen after a week was used to make fungal suspension by adding Milli-Q (ultrapure) water and scratching of colonies upon sterile conditions (Zimmer and Sabourin, 1986;Carrillo et al, 2013).…”

Section: Pathogen Inoculation and Disease Severity Estimationmentioning

confidence: 99%

“…Then, inoculated seedlings were kept under transparent covers during 48 h (Carrillo et al, 2013;Okorska et al, 2014) and after this period, infected seedlings were set apart for one week (12-h photoperiod, 21 ± 2°C) (Garry et al, 1998). Afterward, all seedlings were arranged as described in Performing Experiments and Planting, the aerial parts of infected pea plants by D. pinodes were regularly and periodic investigated by using a USB digital microscope (25X-400X, BMSCI, Japan) to image and computing the lesions (Ranjbar Sistani et al, 2017). The captured dimensions of lesions were applied to calculate the area of lesions and finally disease severity assay (Hwang et al, 2006;Carrillo et al, 2013).…”

Section: Pathogen Inoculation and Disease Severity Estimationmentioning

confidence: 99%

“…In compliance with prior work (Ranjbar Sistani et al, 2017), the total weight of pea seeds per each pot was recorded and the seed yield (kg/ha) formula that was mentioned in Sajid et al (2012) work was adapted to determine the seed yield per treatment (kg pot −1 ). The physical quality of pea seeds was evaluated through quantification of absorption and hardness rates of pea seeds per treatment that were kept in water (16 h) and then were assessed by hydration coefficient (%) and non-soakers (%) formulas (Abdelgani et al, 1999).…”

Section: Investigation Of Physical Properties and Yield Of Pea Seedsmentioning

confidence: 99%

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Seed Metabolism and Pathogen Resistance Enhancement in Pisum sativum During Colonization of Arbuscular Mycorrhizal Fungi: An Integrative Metabolomics-Proteomics Approach

et al. 2020

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Pulses are one of the most important categories of food plants, and Pea (Pisum sativum L.) as a member of pulses is considered a key crop for food and feed and sustainable agriculture. Integrative multi-omics and microsymbiont impact studies on the plant's immune system are important steps toward more productive and tolerant food plants and thus will help to find solutions against food poverty. Didymella pinodes is a main fungal pathogen of pea plants. Arbuscular mycorrhizal fungi (AMF) promote plant growth and alleviate various stresses. However, it remained unclear as to how the AMF effect on seed metabolism and how this influences resistance against the pathogen. This study assesses the AMF impacts on yield components and seed quality upon D. pinodes infection on two different P. sativum cultivars, susceptible versus tolerant, grown in pots through phenotypic and seed molecular analyses. We found that AMF symbiosis affects the majority of all tested yield components as well as a reduction of disease severity in both cultivars. Seeds of mycorrhizal pea plants showed strong responses of secondary metabolites with nutritional, medicinal, and pharmaceutical attributes, also involved in pathogen response. This is further supported by proteomic data, functionally determining those primary and secondary metabolic pathways, involved in pathogen response and induced upon AMF-colonization. The data also revealed cultivar specific effects of AMF symbiosis that increase understanding of genotype related differences. Additionally, a suite of proteins and secondary metabolites are presented, induced in seeds of P. sativum upon AMF-colonization and pathogen attack, and possibly involved in induced systemic resistance against D. pinodes, useful for modern breeding strategies implementing microsymbionts toward increased pathogen resistance.

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Section: Performing Experiments and Plantingmentioning

confidence: 99%

Section: Pathogen Inoculation and Disease Severity Estimationmentioning

confidence: 99%

Section: Pathogen Inoculation and Disease Severity Estimationmentioning

confidence: 99%

Section: Investigation Of Physical Properties and Yield Of Pea Seedsmentioning

confidence: 99%

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Seed Metabolism and Pathogen Resistance Enhancement in Pisum sativum During Colonization of Arbuscular Mycorrhizal Fungi: An Integrative Metabolomics-Proteomics Approach

et al. 2020

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“…The details of proteins known to be either modified and/or altered during the symbiotic process are presented in Figure . Proteomics has provided valuable information regarding the early stages of nodule infection upon the perception of NOD factor as well as subsequent nodulation processes and their response to environmental stimuli, which has been reviewed in depth elsewhere . Due to the dynamics of symbiosis, monitoring and analysis of the cellular and subcellular proteome may give clear ideas as to how symbionts modulate the cell at the different stages of symbiosis .…”

Section: The Role Of Proteomics In Legume–rhizobium Symbiosismentioning

confidence: 99%

Plant–Microbe Symbiosis: What Has Proteomics Taught Us?

et al. 2019

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Beneficial microbes have a positive impact on the productivity and fitness of the host plant. A better understanding of the biological impacts and underlying mechanisms by which the host derives these benefits will help to address concerns around global food production and security. The recent development of omics-based technologies has broadened our understanding of the molecular aspects of beneficial plant-microbe symbiosis. Specifically, proteomics has led to the identification and characterization of several novel symbiosis-specific and symbiosis-related proteins and post-translational modifications that play a critical role in mediating symbiotic plant-microbe interactions and have helped assess the underlying molecular aspects of the symbiotic relationship. Integration of proteomic data with other "omics" data can provide valuable information to assess hypotheses regarding the underlying mechanism of symbiosis and help define the factors affecting the outcome of symbiosis. Herein, an update is provided on the current and potential applications of symbiosis-based "omic" approaches to dissect different aspects of symbiotic plant interactions. The application of proteomics, metaproteomics, and secretomics as enabling approaches for the functional analysis of plant-associated microbial communities is also discussed. 1 of 20) www.advancedsciencenews.com www.proteomics-journal.com to refer to the nodule-forming bacteria of the Rhizobium-and Frankia-type as true symbionts for plants. Other types of beneficial bacteria are referred to as plant growth promoting bacteria (PGPBs), which include endophytes or associative ectophytes that are much more variable in terms of their beneficial contributions. Over the past decades, proteomic approaches have been particularly successful in identifying numerous sets of host and symbiont-related and/or responsive proteins, and components of the signaling factors, which regulate and promote symbiosisrelated processes. Proteomics has also facilitated the discovery of the molecular basis of symbioses initiation and maintenance, and nutrient exchange between the symbiotic partners. (This review focuses on current proteomic applications used to investigate plant-microbe symbiosis such as the large-scale analysis of key regulators of symbiotic-related proteins and their post-translational modifications (PTMs). We also review protein changes at the cellular level, PTMs, and the associated interactions with other molecules during plant symbiotic relationships, with a particular emphasis on the developmental steps of legume-rhizobia symbiosis. The review also links data from proteomics and other "omic" approaches to provide a comprehensive understanding of the molecular basis of plant-microbe symbiosis.

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