Rhizobium mesosinicum sp. nov., isolated from root nodules of three different legumes

Lin, Dong Xu; Chen, Wen Feng; Wang, Feng Qin; Hu, Daogong; Wang, En Tao; Sui, Xin; Chen, Wen Xin

doi:10.1099/ijs.0.006387-0

Cited by 34 publications

(15 citation statements)

References 28 publications

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“…We report here that while the isolates 10L4.2 and 10L5.3 clustered close to the type strain M. plurifarium LGM 11892 that was known to nodulate Acacia spp. and L. leucocephala [43], [44]; isolate 10L11.4 proved to be related to the previously reported R. alamii GBV016T (isolated from the rhizosphere of Arabidopsis thaliana ) [45] and CCBAU15292 (isolated from Albizia julibrissin ) [46], and to R. mesosinicum CCBAU25010 (isolated from Glycine soja ) [47]. These data and the results from the literature taken together indicate that different Desmanthus spp.…”

Section: Discussionsupporting

confidence: 74%

Phenotypic, Molecular and Symbiotic Characterization of the Rhizobial Symbionts of Desmanthus paspalaceus (Lindm.) Burkart That Grow in the Province of Santa Fe, Argentina

et al. 2014

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Desmanthus paspalaceus (Lindm.) Burkart belongs to the D. virgatus complex, subfamily Mimosoidae. The known potential as livestock fodder of several of these legumes prompted us to undertake a phenotypic, molecular, and symbiotic characterization of the D. paspalaceus symbionts in the Santa Fe province, Argentina. The rhizobia collected—containing isolates with different abiotic-stress tolerances—showed a remarkable genetic diversity by PCR fingerprinting, with 11 different amplification profiles present among 20 isolates. In selected isolates 16S-rDNA sequencing detected mesorhizobia (60%) and rhizobia (40%) within the collection, in contrast to the genus of the original inoculant strain CB3126—previously isolated from Leucaena leucocephala—that we typified here through its 16S rDNA as Sinorhizobium terangae. The results revealed the establishment by diverse bacterial genera -rhizobia, sinorhizobia, and mesorhizobia- of full N2-fixing symbiotic associations with D. paspalaceus. This diversity was paralleled by the presence of at least two different nodC allelic variants. The identical nodC alleles of the Mesorhizobia sp. 10.L.4.2 and 10.L.5.3 notably failed to group within any of the currently described rhizo-/brady-/azorhizobial nodC clades. Interestingly, the nodC from S. terangae CB3126 clustered close to homologs from common bean nodulating rhizobia, but not with the nodC from S. terangae WSM1721 that nodulates Acacia. No previous data were available on nod-gene phylogeny for Desmanthus symbionts. A field assay indicated that inoculation of D. paspalaceus with the local Rhizobium sp. 10L.11.4 produced higher aerial-plant dry weights compared to S. teranga CB3126–inoculated plants. Neither the mesorhizobia 10.L.4.2 or 10.L.5.3 nor the rhizobium 10L.11.4 induced root nodules in L. leucocephala or P. vulgaris. The results show that some of the local isolates have remarkable tolerances to several abiotic stresses including acidity, salt, and temperature; while exhibiting prominent N2 fixation; thus indicating suitability as candidates for inoculation of D. paspalaceus.

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Section: Discussionsupporting

confidence: 74%

Phenotypic, Molecular and Symbiotic Characterization of the Rhizobial Symbionts of Desmanthus paspalaceus (Lindm.) Burkart That Grow in the Province of Santa Fe, Argentina

et al. 2014

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“…Mesorhizobium species are usually isolated from root nodules (Lin et al, 2009;Chen et al, 2010). Recently, some species of Mesorhizobium have been linked to sulfur oxidization (Ghosh and Roy, 2006), nitrile biotransformation (Feng et al, 2008), oxamyl degradation (Osborn et al, 2010), high arsenic resistance (Huang A.H. et al, 2010), chromium reducing (Wani et al, 2009), and acetonitrile biodegradation (Feng and Lee, 2009).…”

Section: Discussionmentioning

confidence: 99%

Changes in bacterial community of anthracene bioremediation in municipal solid waste composting soil

Zhang

Wang

Wan

et al. 2011

J. Zhejiang Univ. Sci. B

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Polycyclic aromatic hydrocarbons (PAHs) are common contaminants in a municipal solid waste (MSW) composting site. Knowledge of changes in microbial structure is useful to identify particular PAH degraders. However, the microbial community in the MSW composting soil and its change associated with prolonged exposure to PAHs and subsequent biodegradation remain largely unknown. In this study, anthracene was selected as a model compound. The bacterial community structure was investigated using terminal restriction fragment length polymorphism (TRFLP) and 16S rRNA gene clone library analysis. The two bimolecular tools revealed a large shift of bacterial community structure after anthracene amendment and subsequent biodegradation. Genera Methylophilus, Mesorhizobium, and Terrimonas had potential links to anthracene biodegradation, suggesting a consortium playing an active role.

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“…Current taxonomy showed that all rhizobial species are in the classes of Alpha-and Beta-proteobacteria. Rhizobium, as the first described genus in the alpharhizobia, was named a century ago and now contains 30 symbiotic nitrogen-fixing species including the recently described species: Rhizobium miluonense , Rhizobium oryzae (Peng et al 2008), Rhizobium multihospitium , Rhizobium alamii (Berge et al 2009), Rhizobium tibeticum (Hou et al 2009), Rhizobium alkalisoli (Lu et al 2009), Rhizobium mesosinicum (Lin et al 2009), Rhizobium tubonense , Rhizobium vignae , Rhizobium borbori (Zhang et al 2010a) and Rhizobium herbea (Ren et al 2010b). However, the understanding of species of Rhizobia is far likely to from complete, especially considering the Electronic supplementary material The online version of this article (doi:10.1007/s10482-011-9559-0) contains supplementary material, which is available to authorized users.…”

Section: Introductionmentioning

confidence: 98%

Rhizobium sphaerophysae sp. nov., a novel species isolated from root nodules of Sphaerophysa salsula in China

Shi

Zhao

et al. 2011

Antonie van Leeuwenhoek

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Four gram-negative, aerobic, motile, non-spore, forming rods with a wide pH and temperature range for growth (pH 7.0-11.0, optimum pH 8.0; 20-45°C, optimum 28°C) strains were isolated from root nodules of Sphaerophysa salsula and characterized by means of a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the four strains formed a new lineage related to the genus Rhizobium and the sequence similarities between the isolate and the most related type strain Rhizobium giardinii was 96.5%. These strains also formed a distinctive group from the reference strains for defined Rhizobium species based on housekeeping gene sequences (atpD and recA), BOX-PCR fingerprinting, phenotypic features and symbiotic properties. The representative strain CCNWGS0238(T) has DNA-DNA relatedness of less than 33.4% with the most closely related species R. giardinii. It is therefore proposed as a new species, Rhizobium sphaerophysae sp. nov., with isolate CCNWGS0238(T) (=ACCC17498(T) = HAMBI3074(T)) as the type strain.

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Rhizobium mesosinicum sp. nov., isolated from root nodules of three different legumes

Cited by 34 publications

References 28 publications

Phenotypic, Molecular and Symbiotic Characterization of the Rhizobial Symbionts of Desmanthus paspalaceus (Lindm.) Burkart That Grow in the Province of Santa Fe, Argentina

Phenotypic, Molecular and Symbiotic Characterization of the Rhizobial Symbionts of Desmanthus paspalaceus (Lindm.) Burkart That Grow in the Province of Santa Fe, Argentina

Changes in bacterial community of anthracene bioremediation in municipal solid waste composting soil

Rhizobium sphaerophysae sp. nov., a novel species isolated from root nodules of Sphaerophysa salsula in China

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