Rhizobium symbiosis modulates the accumulation of arsenic in Medicago truncatula via nitrogen and NRT3.1-like genes regulated by ABA and linalool

Ye, Liaoliao; Yang, Peizhi; Zeng, Yongmei; Li, Chun; Jian, Ni; Wang, Ruihua; Huang, Siyuan; Yang, Rongchen; 龙伟,; Zhao, Haiyan; Zheng, Qi; Gao, Huiling; Liu, Jinlong

doi:10.1016/j.jhazmat.2021.125611

Cited by 17 publications

(11 citation statements)

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“…Four‐week‐old seedlings were transplanted to a hydroponic device with an absorbent slope (HDAS) containing different phosphate concentrations for further growth analysis (Ye et al., 2021). Mtvpt2 mutant had significantly higher shoot biomass and lower percentage of yellow and withered leaves than the wild‐type R108 under low phosphate (1 μ m ) concentration (Figure 2d,e).…”

Section: Resultsmentioning

confidence: 99%

“…M. truncatula was cultured as described by Ye et al. (2021). Briefly, M. truncatula (ecotype R108) seeds were sterilized with 75% ethanol, then washed five times using ddH 2 O.…”

Section: Methodsmentioning

confidence: 99%

“…The seedlings were transferred to sponge bars and floated on 1/2 Hoagland nutrient solution. Plants with uniform growth were selected when the seedlings had four to five true leaves for culturing in a hydroponic device with an absorbent slope (HDAS) containing 1/2 Hoagland solution with different phosphate (NaH 2 PO 4 ) concentrations (Ye et al., 2021). The concentration of NO 3 2− in the hydroponic solution was reduced to 2 m m for nodulation.…”

Section: Methodsmentioning

confidence: 99%

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VPT‐like genes modulate Rhizobium–legume symbiosis and phosphorus adaptation

et al. 2023

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SUMMARYAlthough vacuolar phosphate transporters (VPTs) are essential for plant phosphorus adaptation, their role in Rhizobium–legume symbiosis is unclear. In this study, homologous genes of VPT1 (MtVPTs) were identified in Medicago truncatula to assess their roles in Rhizobium–legume symbiosis and phosphorus adaptation. MtVPT2 and MtVPT3 mainly positively responded to low and high phosphate, respectively. However, both mtvpt2 and mtvpt3 mutants displayed shoot phenotypes with high phosphate sensitivity and low phosphate tolerance. The root‐to‐shoot phosphate transfer efficiency was significantly enhanced in mtvpt3 but weakened in mtvpt2, accompanied by lower and higher root cytosolic inorganic phosphate (Pi) concentration, respectively. Low phosphate induced MtVPT2 and MtVPT3 expressions in nodules. MtVPT2 and MtVPT3 mutations markedly reduced the nodule number and nitrogenase activity under different phosphate conditions. Cytosolic Pi concentration in nodules was significantly lower in mtvpt2 and mtvpt3 than in the wildtype, especially in tissues near the base of nodules, probably due to inhibition of long‐distance Pi transport and cytosolic Pi supply. Also, mtvpt2 and mtvpt3 could not maintain a stable cytosolic Pi level in the nodule fixation zone as the wildtype under low phosphate stress. These findings show that MtVPT2 and MtVPT3 modulate phosphorus adaptation and rhizobia–legume symbiosis, possibly by regulating long‐distance Pi transport.

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Section: Resultsmentioning

confidence: 99%

“…M. truncatula was cultured as described by Ye et al. (2021). Briefly, M. truncatula (ecotype R108) seeds were sterilized with 75% ethanol, then washed five times using ddH 2 O.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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VPT‐like genes modulate Rhizobium–legume symbiosis and phosphorus adaptation

et al. 2023

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“…This has been demonstrated in faba bean plants, where a combination of Bacillus strains with those of Rhizobium increased the biosorption of Ni and improved plant growth [103]. Similarly, co-inoculation of rhizobia and non-rhizobial bacteria has been shown to improve tolerance against Pb [104], As [94,105], and Cu [106,107]. The tolerance mechanism is not well understood, but metal biosorption by rhizobia, accumulation of metals in the roots, volatilization, conversion to less toxic forms, and rhizobia-induced root uptake regulation are some of the ways the symbionts are known to alleviate the metal toxicity.…”

Section: Environmental Adaptabilitymentioning

confidence: 97%

“…Nevertheless, some legumes can effectively nodulate and grow in HMCS, suggesting a protective effect of rhizobial symbionts against many of these metals. The Rhizobiuminduced improvements in nodulation, root and shoot lengths, N-fixation, and seed yield were observed in legumes grown in soil contaminated with Ni (290 mg Ni kg −1 soil) [91], As [92][93][94], Hg [95], Cd [96], Cr [97], Cu [98], Al [99], and Pb [100].…”

Section: Environmental Adaptabilitymentioning

confidence: 99%

The Plant-Rhizobial Symbiotic Interactions Provide Benefits to the Host beyond Nitrogen Fixation That Promote Plant Growth and Productivity

Habtewold¹,

Goyal²

2023

Symbiosis in Nature

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Rhizobial symbiotic interactions are known for nitrogen fixation, providing commercial crops and other plants with self-sufficiency in nitrogen requirements. An enormous contribution from nitrogen fixation is vital to the global nitrogen cycle. The symbiotic nitrogen reduces the carbon footprint of crop cultivation, which underlines its importance in agricultural sustainability. Extensive research efforts have been made to understand the symbiotic relationship at molecular, physiological, and ecological levels. This led to the isolation and modification of symbiotic strains for enhanced nitrogen efficiency. During the evaluation of strains for nitrogen fixation in exchange for supporting the bacterium in terms of space and resources, it has been observed that the accrued benefits to the host plants extend well beyond the nitrogen fixation. The symbiotic interaction has been advantageous to the host for better growth and development, tolerating a stressful environment, and even keeping the pathogenic microbial enemies at bay. Additionally, it enabled the availability of the mineral nutrients, which otherwise were inaccessible to the host. In this chapter, we bring together the information with a focus on the role of rhizobial symbiotic interactions that promote plant growth and productivity through phytohormone synthesis, by facilitating the availability of mineral nutrients, and by improving the plant tolerance to sub-optimal growth conditions.

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Nodulation Signaling Pathway 1 and 2 Modulate Vanadium Accumulation and Tolerance of Legumes

Liu,

Zhang,

Lin

et al. 2024

Advanced Science

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Vanadium (V) pollution potentially threatens human health. Here, it is found that nsp1 and nsp2, Rhizobium symbiosis defective mutants of Medicago truncatula, are sensitive to V. Concentrations of phosphorus (P), iron (Fe), and sulfur (S) with V are negatively correlated in the shoots of wild‐type R108, but not in mutant nsp1 and nsp2 shoots. Mutations in the P transporter PHT1, PHO1, and VPT families, Fe transporter IRT1, and S transporter SULTR1/3/4 family confer varying degrees of V tolerance on plants. Among these gene families, MtPT1, MtZIP6, MtZIP9, and MtSULTR1;1 in R108 roots are significantly inhibited by V stress, while MtPHO1;2, MtVPT2, and MtVPT3 are significantly induced. Overexpression of Arabidopsis thaliana VPT1 or M. truncatula MtVPT3 increases plant V tolerance. However, the response of these genes to V is weakened in nsp1 or nsp2 and influenced by soil microorganisms. Mutations in NSPs reduce rhizobacterial diversity under V stress and simplify the V‐responsive operational taxonomic unit modules in co‐occurrence networks. Furthermore, R108 recruits more beneficial rhizobacteria related to V, P, Fe, and S than does nsp1 or nsp2. Thus, NSPs can modulate the accumulation and tolerance of legumes to V through P, Fe, and S transporters, ion homeostasis, and rhizobacterial community responses.

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Rhizobium symbiosis modulates the accumulation of arsenic in Medicago truncatula via nitrogen and NRT3.1-like genes regulated by ABA and linalool

Cited by 17 publications

References 77 publications

VPT‐like genes modulate Rhizobium–legume symbiosis and phosphorus adaptation

VPT‐like genes modulate Rhizobium–legume symbiosis and phosphorus adaptation

The Plant-Rhizobial Symbiotic Interactions Provide Benefits to the Host beyond Nitrogen Fixation That Promote Plant Growth and Productivity

Nodulation Signaling Pathway 1 and 2 Modulate Vanadium Accumulation and Tolerance of Legumes

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