Rhizogenic agrobacteria as an innovative tool for plant breeding: current achievements and limitations

Desmet, Siel; Dhooghe, Emmy; Keyser, Ellen De; Huylenbroeck, Johan Van; Müller, Renate; Geelen, Danny; Lütken, Henrik

doi:10.1007/s00253-020-10403-7

Cited by 38 publications

(39 citation statements)

References 162 publications

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“…Similarly, pRi transformation of Solanum tuberosum led to oblong tubers more with prominent eyes (Ooms et al, 1985;Ondřej et al, 1989). The Ri phenotype is also known to encompass changes related to flowering time and flower morphology (Desmet et al, 2020). Both S. speciosa Ri lines exhibited delayed flowering, a feature also reported in Ri lines of Ipomoea trichocarpa, Kalanchoe blossfeldiana, and Lavandula x intermedia (Otani et al, 1996;Christensen et al, 2008;Tsuro and Ikedo, 2011).…”

Section: Discussionmentioning

confidence: 83%

“…Changes in compactness are commonly attributed to changes of more than one specific organ (Desmet et al, 2020). In Hypericum perforatum, Ri lines were obtained in which the entire shoot was miniaturized (Bertoli et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Alternative techniques that reduce or eliminate the need for chemical growth regulation have been evaluated with promising results (Lütken et al, 2012a). Natural transformation of plants with wild type rhizogenic agrobacteria is a technique, already applied for several ornamental plant species, that resulted in plants with a more compact growth habit (Desmet et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Common traits of this phenotype include dwarfing, increased branching, wrinkled leaves, decreased apical dominance and enhanced root growth (Tepfer, 1984). Characteristics of the Ri phenotype that are potentially useful for the ornamental industry have recently been reviewed in Desmet et al (2020). The Ri phenotype is not fixed and may vary depending on the individual transformation event, the positioning of the T-DNA integration in the plant genome, the T-DNA copy number and eventual fragmentation and expression levels of the associated aux (auxin biosynthesis genes) and rol (root oncogenic loci) genes.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, it has been shown that the rol genes of the Ri plasmid can be stably transmitted, by sexual means, to the progeny ( Tepfer, 1984 ; Lütken et al, 2012c ). The combination of several economically important traits, each with a range of natural variation and compatibility with existing breeding programs, favors the use of Ri lines in plant breeding to facilitate and accelerate the progress toward sustainable compact plant growth ( Desmet et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Segregation of rol Genes in Two Generations of Sinningia speciosa Engineered Through Wild Type Rhizobium rhizogenes

et al. 2020

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Rhizobium rhizogenes infects and transforms a wide range of plant species. It thereby introduces new genes located on transfer-DNA of the root inducing plasmid (pRi) into the plant genome and one of its abilities is to alter the host root system. Explants from pRi transformed roots from Sinningia speciosa were regenerated to create naturally transgenic Ri lines. The presence of rol and aux genes in the Ri lines was linked with altered growth characteristics: shorter peduncles, wrinkled leaves, delayed flowering and enhanced root growth. The potential of Ri lines for breeding was evaluated through consecutive backcrossing with the original host genotype. The progeny of reciprocal crosses showed non-Mendelian inheritance suggesting partial transmission of the of the aux and rol genes. The typical Ri phenotype observed in the primary Ri line was partially inherited. These results revealed that the Ri phenotype is a complex trait influenced by the genetic background of the Ri line.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Segregation of rol Genes in Two Generations of Sinningia speciosa Engineered Through Wild Type Rhizobium rhizogenes

et al. 2020

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The genome sequence of hairy root Rhizobium rhizogenes strain LBA9402: Bioinformatics analysis suggests the presence of a new opine system in the agropine Ri plasmid

Hooykaas

2021

MicrobiologyOpen

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Hairy root, a neoplastic plant disease with a wide host range, is characterized by the formation of adventitious roots from infected wound sites (Riker, 1930). It was originally encountered as a problem in tree nurseries, but nowadays the disease is also increasingly causing problems in the greenhouse by inducing extensive root mats, thereby reducing the harvest of cucumbers and tomatoes (Weller et al., 2000). The causal agent, a bacterium that was for long called Agrobacterium rhizogenes, contains a large, about 200 kbp

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The rolB‐transgenic Nicotiana tabacum plants exhibit upregulated ARF7 and ARF19 gene expression

et al. 2022

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Agrobacterium rhizogenes root oncogenic locus B (rolB) is known to induce hairy roots along with triggering several physiological and morphological changes when present as a transgene. However, it is still unknown how this gene triggers these changes within the plant system. In this study, the effect of rolB in-planta, when present as a transgene, was assessed on the gene expression levels of auxin response factors (ARFs)-transcription factors which are key players in auxin-mediated responses. The goal was to uncover Auxin/ARF-driven transcriptional networks potentially active and working selectively, if any, in rolB transgenic background, which might potentially be associated with hairy root development. Hence, the approach involved establishing rolB-transgenic Nicotiana tabacum plants, selecting ARFs (NtARFs) for context-relevance using bioinformatics followed by gene expression profiling. It was observed that out of the chosen NtARFs, NtARF7 and NtARF19 exhibited a consistent pattern of gene upregulation across organ types. In order to understand the significance of these selective gene upregulation, ontology-based transcriptional network maps of the differentially and nondifferentially expressed ARFs were constructed, guided by co-expression databases. The network maps suggested that NtARF7-NtARF19 might have major deterministic, underappreciated roles to play in root development in a rolB-transgenic background-as observed by higher number of "root-related" biological processes present as nodes compared to network maps for similarly constructed other non-differentially expressed ARFs. Based on the inferences drawn, it is hypothesized that rolB, when present as a transgene, might drive hairy root development by selective induction of NtARF7 and NtARF19, suggesting a functional link between the two, leading to the specialized and characteristic rolBassociated traits.

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Rhizogenic agrobacteria as an innovative tool for plant breeding: current achievements and limitations

Cited by 38 publications

References 162 publications

Segregation of rol Genes in Two Generations of Sinningia speciosa Engineered Through Wild Type Rhizobium rhizogenes

Segregation of rol Genes in Two Generations of Sinningia speciosa Engineered Through Wild Type Rhizobium rhizogenes

The genome sequence of hairy root Rhizobium rhizogenes strain LBA9402: Bioinformatics analysis suggests the presence of a new opine system in the agropine Ri plasmid

The rolB‐transgenic Nicotiana tabacum plants exhibit upregulated ARF7 and ARF19 gene expression

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