Rhizoma smilacis glabrae protects rats with gentamicin-induced kidney injury from oxidative stress-induced apoptosis by inhibiting caspase-3 activation

Liu, Cuiyan; Kang, Youxi; Zhou, Xiuhong; Yang, Zhiming; Gu, Jingang; Han, Chunyang

doi:10.1016/j.jep.2016.12.034

Cited by 16 publications

(10 citation statements)

References 22 publications

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“…Baicalin, a flavonoid widely used to treat infectious and inflammatory diseases, also inhibited renal cell apoptosis in a mouse model of sepsis produced by cecal ligation and puncture (Zhu et al, 2016 ) through inhibition of the pro-apototic regulator BAX. Rats with gentamicin-induced kidney injury were also protected from oxidative stress-induced apoptosis by aqueous extracts of Rhizoma smilacis glabrae through inhibition of proapoptotic caspase-3 activation (Liu et al, 2017 ).…”

Section: Renal Apoptosis and Fibrosismentioning

confidence: 99%

Flavonoids in Kidney Health and Disease

et al. 2018

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This review summarizes the latest advances in knowledge on the effects of flavonoids on renal function in health and disease. Flavonoids have antihypertensive, antidiabetic, and antiinflammatory effects, among other therapeutic activities. Many of them also exert renoprotective actions that may be of interest in diseases such as glomerulonephritis, diabetic nephropathy, and chemically-induced kidney insufficiency. They affect several renal factors that promote diuresis and natriuresis, which may contribute to their well-known antihypertensive effect. Flavonoids prevent or attenuate the renal injury associated with arterial hypertension, both by decreasing blood pressure and by acting directly on the renal parenchyma. These outcomes derive from their interference with multiple signaling pathways known to produce renal injury and are independent of their blood pressure-lowering effects. Oral administration of flavonoids prevents or ameliorates adverse effects on the kidney of elevated fructose consumption, high fat diet, and types I and 2 diabetes. These compounds attenuate the hyperglycemia-disrupted renal endothelial barrier function, urinary microalbumin excretion, and glomerular hyperfiltration that results from a reduction of podocyte injury, a determinant factor for albuminuria in diabetic nephropathy. Several flavonoids have shown renal protective effects against many nephrotoxic agents that frequently cause acute kidney injury (AKI) or chronic kidney disease (CKD), such as LPS, gentamycin, alcohol, nicotine, lead or cadmium. Flavonoids also improve cisplatin- or methotrexate-induced renal damage, demonstrating important actions in chemotherapy, anticancer and renoprotective effects. A beneficial prophylactic effect of flavonoids has been also observed against AKI induced by surgical procedures such as ischemia/reperfusion (I/R) or cardiopulmonary bypass. In several murine models of CKD, impaired kidney function was significantly improved by the administration of flavonoids from different sources, alone or in combination with stem cells. In humans, cocoa flavanols were found to have vasculoprotective effects in patients on hemodialysis. Moreover, flavonoids develop antitumor activity against renal carcinoma cells with no toxic effects on normal cells, suggesting a potential therapeutic role in patients with renal carcinoma.

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Section: Renal Apoptosis and Fibrosismentioning

confidence: 99%

Flavonoids in Kidney Health and Disease

et al. 2018

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“…To further investigate the mechanism of the extract's inhibitory effect on apoptosis, signaling cascades involving the caspase pathway, specifically caspase-3, caspase-8, caspase-9, Bax, and Bcl-2 were measured [11]. It has been proposed that the activating caspases, caspase-3, -8, and -9, serve as biochemical hallmarks of apoptosis [24]. The extract inactivated the expression of caspase-3 and 8, markedly at 24 h after treatment.…”

Section: Discussionmentioning

confidence: 99%

<i>Artemisia anomala</i> extracts enhance the viability and antioxidation capacity of human keratinocytes

Gao¹,

Yuan²,

Liang³

et al. 2019

Trop. J. Pharm Res

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Purpose: To investigate the effect of extracts of Artemisia anomala S. Moore tissues on viability, apoptosis and antioxidant capacity of human keratinocytes. Methods: Human keratinocyte cell line HaCaT were treated with extracts of A. anomala for 12 h or 24 h. Cell viability, level of reactive oxygen species (ROS), and incidence of apoptosis were measured by flow cytometry. Levels of mRNA and key proteins in the mitogen-activated protein kinase (MAPK) pathway were determined by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Key proteins of caspase pathways were assessed by western blot. The influence of the extract on the MAPK pathway was further probed by treating cells with MAPK activator in the presence and absence of the extract. Results: Treatment of cells with extracts of A. anomala enhanced viability and reduced apoptosis in a time-dependent manner, and increased ROS level, compared with control. mRNA and protein expressions of c-Jun N-terminal kinase (JNK), extracellular regulated protein kinase (ERK), and p38 MAPK decreased in extract-treated cells. The extracts also reversed the inhibitory effects of the MAPK pathway activator, actinomycin, on cell viability and ROS, and inhibited protein-cleaved caspase-8 and cleaved caspase-3. Conclusion: A. anomala extract increases cell viability and antioxidant capacity via inactivation of MAPK pathway, and also inhibits cell apoptosis via inactivation of caspase pathways. Hence, the extract may serve as a promising drug for the treatment of psoriasis.

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“…Furthermore, oxidative stress is known as the main mechanism involved in many toxicological procedures and contributed to immunotoxicity of various agents. 29 For example, relation between oxidative stress and immunotoxicity following exposure to cadmium was shown in zebra fish. 30 Also, previous studies reported increased oxidative stress and decreased antioxidant capacity in some ACR toxicological effects, such as neurotoxicity, reproductive, and genotoxicity.…”

Section: Discussionmentioning

confidence: 99%

Acrylamide attenuated immune tissues’ function via induction of apoptosis and oxidative stress: Protection by l-carnitine

et al. 2017

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Acrylamide (ACR), with high prevalence in starchy food, has been associated with the development of several organ toxicities such as immunotoxicity. This study aimed to demonstrate the role of oxidative stress and apoptosis as the mechanisms involved in ACR-induced immunotoxicity in mice. Mice were randomly assigned to six groups and treated as follows: control (normal saline), cyclophosphamide (200 mg kg), ACR groups (12.5, 25 and 50mg kg, orally), and l-carnitine (l-CAR; 100 mg kg) + ACR (50 mg kg). After 30 days of exposure, mice were killed and immunotoxic response was evaluated via immune blood cells count and body/organ weights. Oxidative stress parameters and pathological examination were done in thymus and spleen. Also, the apoptosis was evaluated via flow cytometric by annexin V/FITC kit in the splenocytes. Our results indicated that ACR could induce immunotoxicity characterized by reduction in immune blood cells, body/organ weights, and pathological changes in spleen. The assessment of oxidative stress markers revealed increase in lipid peroxidation, protein carbonyl content, and depletion of glutathione level. Also, increased apoptosis was observed in splenocytes after ACR administration compared to the control group. These alterations were markedly normalized by coadministration of l-CAR (as a potent antioxidant). Taken together, the results of this study showed the potential of ACR to induce immunotoxicity through provoking oxidative stress and inducing apoptosis and the protective effect of l-CAR to attenuate this toxicity. These findings will help in elucidating the toxicity mechanism induced by ACR.

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Rhizoma smilacis glabrae protects rats with gentamicin-induced kidney injury from oxidative stress-induced apoptosis by inhibiting caspase-3 activation

Cited by 16 publications

References 22 publications

Flavonoids in Kidney Health and Disease

Flavonoids in Kidney Health and Disease

<i>Artemisia anomala</i> extracts enhance the viability and antioxidation capacity of human keratinocytes

Acrylamide attenuated immune tissues’ function via induction of apoptosis and oxidative stress: Protection by l-carnitine

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