Rhizopus arrhizus lipase-catalyzed interesterification of palm oil midfraction in a gas-lift reactor

Mojović, Ljiljana; Šiler-Marinković, Slavica; Kukić, Gojko; Bugarski, Branko; Vunjak‐Novakovic, Gordana

doi:10.1016/0141-0229(94)90079-5

Cited by 8 publications

(1 citation statement)

References 11 publications

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“…Thus, use of an immobilized lipase (PSL-Celite) with high activity in solid-phase reactions (glycerolysis and esterification) has been described in this paper. From these results and those reported for interesterification processes (33,34), diatomaceous earth (Hyflo Supercel and Celite) has been shown to be useful in biotransformations of fats and oils. Comparisons of the conversions, yields, and costs in this paper suggest that, while glycerolysis is the most interesting process to produce DO, esterification is better for MO preparation with this biocatalyst.…”

Section: Effect Of Enzyme Concentration On Conversionsupporting

confidence: 59%

Enzyme, medium, and reaction engineering to design a low‐cost, selective production method for mono‐ and dioleoylglycerols

Arcos

Otero

1996

J Americ Oil Chem Soc

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The selective enzymic production of mono-and diolein (MO, DO) was optimized at high yields. A comparative study of the following distinct enzymic reactions was conducted: ethyl oleate glycerolysis, triolein (TO) glycerolysis, and direct esterification. Solvent-free systems were compared with media that contained different solvents. Native, modified (with polyethylene glycol), and immobilized lipases were used. Mechanical resistance, the support effect on enzyme and glycerol dispersion and on process reproducibility, and hydrophilicity of the support were considered in the process optimization. We report the use of an immobilized lipase on an inorganic support (Celite), which has high activities in both solid-phase glycerolysis (99% reaction conversion) and esterification (100% conversion). The optimum conditions for the distinct reactions were compared by considering their selectivities, conversions, yields, and cost of the substrates. We found less costly and more selective processes in the absence of solvents for glycerolysis of triolein and direct esterification. Although glycerolysis was the most interesting process to produce diolein, esterification was better for monoolein preparation with this biocatalyst. The esterification reaction yielded 93 wt% of MO, in the absence of either TO or oleic acid (OA), at low cost because of the 100% reaction conversion. Similar costs of the substrates (10.6 and 10.1 S/g) were necessary to obtain 67 and 80 wt% of DO in esterification and glycerolysis, respectively. The glycerolysis conversion was 96%. In esterification, the product mixture was impure, with a high amount of residual OA due to the low conversion (59%). The high activity of PSL-Celite in these solid-phase reactions has an advantage over the reactions with nonimmobilized lipases due to the ease of enzyme recovery. The absence of organic solvents reduces the need for solvent removal from the reaction mixtures.JAOCS 73, 673-682 (1996).

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Section: Effect Of Enzyme Concentration On Conversionsupporting

confidence: 59%

Enzyme, medium, and reaction engineering to design a low‐cost, selective production method for mono‐ and dioleoylglycerols

Arcos

Otero

1996

J Americ Oil Chem Soc

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Production of specific-structured triacylglycerols by lipase-catalyzed reactions: a review

2000

Eur. J. Lipid Sci. Technol.

321

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Regiospecificity is one of the major advantages of using lipase technology for the modification of oils and fats to produce high‐value added products, such as cocoa butter equivalents, human milk fat substitutes, and other specific‐structured lipids. Due to the high cost of biocatalysts, the mainstream applications of lipases for normal oils and fats are still limited. Therefore, positional specificity of lipases has the priority and will be the target property to be exploited for commercial and industrial developments, because no chemical method has such a specificity and is promising or possible for this task. In this paper, encouraging products resulting from this regiospecificity are reviewed together with the critical evaluation of their reaction schemes, side reactions and by‐products, sources of substrate oils and acyl donors, and production processes.

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Principles of Bioreactor Design for Encapsulated Cells

Bugarski¹,

Goosen²,

Vunjak‐Novakovic³

1999

Cell Encapsulation Technology and Therapeutics

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Rhizopus arrhizus lipase-catalyzed interesterification of palm oil midfraction in a gas-lift reactor

Cited by 8 publications

References 11 publications

Enzyme, medium, and reaction engineering to design a low‐cost, selective production method for mono‐ and dioleoylglycerols

Enzyme, medium, and reaction engineering to design a low‐cost, selective production method for mono‐ and dioleoylglycerols

Production of specific-structured triacylglycerols by lipase-catalyzed reactions: a review

Principles of Bioreactor Design for Encapsulated Cells

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