Rhizopus oligosporus and yeast co-cultivation during barley tempeh fermentation—Nutritional impact and real-time PCR quantification of fungal growth dynamics

Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production

“…The population of yeasts and LAB for tempeh production were dynamic over time and differed between the two methods of tempeh production. Regardless the method of tempeh production, the occurrence of yeast during tempeh production was reported by Ashenafi and Busse (1991);Feng et al (2007). However the studied the not on the yeast dynamic during tempeh production.…”

“…However the studied the not on the yeast dynamic during tempeh production. Yeast population were detected in water of soaking soybean nearly reach 10 6 cfu/ml in acidified water and 10 8 cfu/ml in unacidified water (Ashenafi & Busse 1991) and numerous yeast species grew in barlay tempeh (Feng et al 2007). This study found the yeast population was maximum at final product and amounted to 9.70 log cfu/g in tempeh production method A and 6.85 log cfu/g in method B.…”

“…At stage T5, these yeast and LAB phylotypes were dominated by a few phylotypes. These phylotypes found at T5 were phylotypes that could survive from various previous treatments, including their ability to grow together with mold dominated by Rhizopus oligosporus, their biochemical ability to use soybean component from catabolic products or mold metabolism products, including the production of antimicrobe (Mulyowidarso et al 1990;Feng et al 2005Feng et al , 2007.…”

“…Nout and Kiers (2005) stated that these two microbes were commonly found in tempeh. Yeast has not been studied comprehensively during soybean tempeh production, but Feng et al (2007) report numerous yeast species grew in barley tempeh. LAB were found in the raw materials and during production of tempeh in home industry in Malaysia (Moreno et al 2002), starting from raw materials, in the stage of soybean soaking, soybean boiling and in fresh tempeh.…”

“…The role of yeast during tempeh production is still unclear. Feng et al (2005Feng et al ( , 2007 showed that in barley tempeh, the inoculation of yeasts and LAB with R. oligosporus simultaneously in certain concentration does not inhibit the growth of R. oligosporus.…”