Rhizospheric microbiomes of Sorghum bicolor grown on soils with anthropogenic polyelement anomalies

Abstract

Comparative study was made of the rhizospheree microbiomes of two cultivars of sorghum (Sorghum bicolor cvs. Sucro 506 and Biomass 133) grown on soils with anthropogenic polyelement anomalies and on a background (control) soil. The study used traditional culture-based and culture-independent metagenomic approaches. In soils contaminated with heavy metals, we found decreased numbers of culturable bacteria and quantitative changes in the populations of actinomycetes and micromycetes. The relative abundance of the families whose members were able to resist heavy metals was found to increase in the rhizospheric communities. The taxonomic profile of the microbial communities at the phylum level did not differ significantly between cultivars. The Shannon diversity and the abundance of actinomycete families in the rhizosphere of cv. Biomass 133were greater than those for cv. Sucro 506. Significant differences were found between cultivars for the number of rhizospheric microorganisms resistant to heavy metals.

Effect of organic and conventional farming on soil bacterial diversity of pecan tree ( Carya illinoensis K. Kosh) orchard across two phenological stages

“…3a,b), have been reported as rhizospheric actinobacteria (Muratova et al . 2020) and their abundance being affected by soil disturbance (Shange et al . 2012) and the presence of pollutant (Köhler et al .…”

Defined synthetic microbial communities colonize and benefit field-grown sorghum

“…In this study, we aimed to design a defined SynCom (dSynCom) with the ultimate goal of testing engraftment and impact on host phenotype in the bioenergy, feed, and forage crop Sorghum bicolor. Sorghum serves as an excellent host for this work as there exists extensive sequencingbased characterization of its microbiome under a range of abiotic conditions and environments (Xu et al, 2018;Muratova et al, 2020;Yukun et al, 2021;Abera et al, 2022;Qi et al, 2022). Using these datasets, and a culture collection of sorghum root-derived bacterial isolates, we selected a total of 57 strains following two complementary but orthogonal guiding principles for inclusion: 1) network-analysis based identification of strains related to abundant and common hub taxa within the sorghum rhizosphere microbiome, and 2) identification of stains capable of using sorghumspecific root exudate compounds as a carbon source.…”