Rhodamine 123 uptake and mitochondrial DNA content in rabbit articular chondrocytes evolve differently upon transfer from cartilage to culture conditions

Champagne, Anne‐Marie; Benel, Laurent; Ronot, Xavier; Mignotte, Françoise; Adolphe, M

doi:10.1016/0014-4827(87)90172-8

Cited by 17 publications

(21 citation statements)

References 9 publications

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“…First, chondrocyte phenotype and gene expression change significantly when cells are isolated from the native extracellular matrix and cultured in monolayer . In one study, MT metabolism and MT biogenesis were drastically altered in both primary cultured (i.e., non‐passaged) and first‐passage chondrocytes . Second, accurate modeling of mechanical trauma to cartilage requires the native extracellular matrix.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial dysfunction is an acute response of articular chondrocytes to mechanical injury

Delco

Bonnevie

Bonassar

et al. 2017

Journal Orthopaedic Research

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Section: Discussionmentioning

confidence: 99%

Mitochondrial dysfunction is an acute response of articular chondrocytes to mechanical injury

Delco

Bonnevie

Bonassar

et al. 2017

Journal Orthopaedic Research

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“…In comparison with those of other cell types, the mitochondrial activity of chondrocytes seems to be very particular (8). Effectively, chondrocytes maintain in vivo a predominantly fermentative metabolism, with a low level of respiration (oxygen consumption 20 to 50 times reduced, compared with that of other tissues).…”

mentioning

confidence: 98%

Cytofluorometric analysis of chondrotoxicity of fluoroquinolone antimicrobial agents

Hayem

Petit

Levacher

et al. 1994

Antimicrob Agents Chemother

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To better understand quinolone-related arthropathy, we conceived an experimental ex vivo model using cell cultures of articular chondrocytes issued from pretreated New Zealand White rabbits (NZW). Juvenile (4-to 5-week-old) NZW were orally dosed with ofloxacin or pefloxacin (300 mg/kg of body weight for 1 day) or with pefloxacin (300 mg/kg for 7 days). Adult (5-month-old) NZW were treated with pefloxacin (300 mg/kg for 1 day). Chondrocytes were enzymatically recovered from cartilage and were analyzed by cytofluorometry using 2',7'-dichlorofluorescein diacetate (DCFH-DA) and dihydrorhodamine 123 (DHR), reflecting cellular respiratory-burst activity, and rhodamine 123 (Rh123) and 10-N-nonyl-acridine orange (NAO), specific for the mitochondrial activity and mass, respectively. A significant increase in the respiratory burst was detected by DCFH-DA and DHR in all treated groups of young animals, compared with untreated control groups. No significant increase of respiratory burst was noted in older treated rabbits. The 7-day treatment resulted in a decrease in mitochondrial uptake of Rh123 and an increase in NAO uptake. Fluoroquinolone arthrotoxicity seems to involve in its early phase the respiratory burst of immature articular chondrocytes.

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“…The oxygen consumption rate of articular chondrocytes is many‐fold lower than that of other cell types (Bywaters, 1937; Rosenthal et al, 1941; Heywood et al, 2006). The exceptionally low oxygen consumption rate of chondrocytes parallels the low mitochondrial content of these cells (Brighton et al, 1984; Champagne et al, 1987; Eggli et al, 1988). Chondrocytes derive ∼95% of their energy from glycolysis (Lee and Urban, 1997; Heywood and Lee, 2008), which does not require oxygen for ATP generation.…”

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confidence: 99%

Both superficial and deep zone articular chondrocyte subpopulations exhibit the crabtree effect but have different basal oxygen consumption rates

Heywood

Knight

Lee

2010

Journal Cellular Physiology

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In the absence of in vivo measurements, the oxygen concentration within articular cartilage is calculated from the balance between cellular oxygen consumption and mass transfer. Current estimates of the oxygen tension within articular cartilage are based on oxygen consumption data from full-depth tissue samples. However, superficial and deep cell subpopulations of articular cartilage express intrinsic metabolic differences. We test the hypothesis that the subpopulations differ with respect to their intrinsic oxygen consumption rate. Chondrocytes from the full cartilage thickness demonstrate enhanced oxygen consumption when deprived of glucose, consistent with the Crabtree phenomena. Chondrocyte subpopulations differ in the prevailing availability of oxygen and glucose, which decrease with distance from the cartilage-synovial fluid interface. Thus, we tested the hypothesis that the oxygen consumption of each subpopulation is modulated by nutrient availability, by examining the expression of the Crabtree effect. The deep cells had a greater oxygen consumption than the superficial cells (V(max) of 6.6 compared to 3.2 fmol/cell/h), consistent with our observations of mitochondrial volume (mean values 52.0 vs. 36.4 microm(3)/cell). Both populations expressed the Crabtree phenomena, with oxygen consumption increasing approximately 2.5-fold in response to glycolytic inhibition by glucose deprivation or 2-deoxyglucose. Over 90% of this increase was oligomycin-sensitive and thus accounted for by oxidative phosphorylation. The data contributes towards our understanding of chondrocyte energy metabolism and provides information valuable for the accurate calculation of the oxygen concentration that the cells experience in vivo. The work has further application to the optimisation of bioreactor design and engineered tissues.

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Rhodamine 123 uptake and mitochondrial DNA content in rabbit articular chondrocytes evolve differently upon transfer from cartilage to culture conditions

Cited by 17 publications

References 9 publications

Mitochondrial dysfunction is an acute response of articular chondrocytes to mechanical injury

Mitochondrial dysfunction is an acute response of articular chondrocytes to mechanical injury

Cytofluorometric analysis of chondrotoxicity of fluoroquinolone antimicrobial agents

Both superficial and deep zone articular chondrocyte subpopulations exhibit the crabtree effect but have different basal oxygen consumption rates

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