Rhodamine B-conjugated encrypted vipericidin nonapeptide is a potent toxin to zebrafish and associated with in vitro cytotoxicity

Wang, Liang; Chan, Judy Yuet-Wa; Rêgo, Juciane Vaz; Chong, Cheong-Meng; Ai, Nana; Falcão, Cláudio Borges; Rádis‐Baptista, Gandhi; Lee, Shuncheng

doi:10.1016/j.bbagen.2015.02.013

Cited by 26 publications

(17 citation statements)

References 48 publications

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“…Pb-CATH1 contains the previously reported amphipathic nonapeptide, KRFKKFFKK, which is nearly identical in all snake venom cathelin-related antimicrobial peptides (svCRAMPs), including crotalicidin, lachesicidin, batroxicidin, lutzicidin, BF-CRAMP, OH-CRAMP, NA-CRAMP, Pt-CRAMP1, and Pt-CRAMP2 (19,31,32) (see Fig. S2 in the supplemental material), suggesting that Pb-CATH1 is a member of a cathelicidin family common to snakes.…”

Section: Discussionmentioning

confidence: 87%

Genomewide Analysis of the Antimicrobial Peptides in Python bivittatus and Characterization of Cathelicidins with Potent Antimicrobial Activity and Low Cytotoxicity

Kim

Soundrarajan

Lee

et al. 2017

Antimicrob Agents Chemother

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In this study, we sought to identify novel antimicrobial peptides (AMPs) in through bioinformatic analyses of publicly available genome information and experimental validation. In our analysis of the python genome, we identified 29 AMP-related candidate sequences. Of these, we selected five cathelicidin-like sequences and subjected them to further analyses. The results showed that these sequences likely have antimicrobial activity. The sequences were named Pb-CATH1 to Pb-CATH5 according to their sequence similarity to previously reported snake cathelicidins. We predicted their molecular structure and then chemically synthesized the mature peptide for three putative cathelicidins and subjected them to biological activity tests. Interestingly, all three peptides showed potent antimicrobial effects against Gram-negative bacteria but very weak activity against Gram-positive bacteria. Remarkably, ΔPb-CATH4 showed potent activity against antibiotic-resistant clinical isolates and also was observed to possess very low hemolytic activity and cytotoxicity. ΔPb-CATH4 also showed considerable serum stability. Electron microscopic analysis indicated that ΔPb-CATH4 exerts its effects via toroidal pore preformation. Structural comparison of the cathelicidins identified in this study to previously reported ones revealed that these Pb-CATHs are representatives of a new group of reptilian cathelicidins lacking the acidic connecting domain. Furthermore, Pb-CATH4 possesses a completely different mature peptide sequence from those of previously described reptilian cathelicidins. These new AMPs may be candidates for the development of alternatives to or complements of antibiotics to control multidrug-resistant pathogens.

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Section: Discussionmentioning

confidence: 87%

Genomewide Analysis of the Antimicrobial Peptides in Python bivittatus and Characterization of Cathelicidins with Potent Antimicrobial Activity and Low Cytotoxicity

Kim

Soundrarajan

Lee

et al. 2017

Antimicrob Agents Chemother

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“…; Wang, Chang et al . ). The sequence and structure of ABPs are complicated, and several universal traits have been noted among different species.…”

Section: Biological Factorsmentioning

confidence: 97%

“…The quality of water within an aquaculture system is an important issue, and the appropriate concentration of chitosan increases the transparency of water due to its adsorption and flocculation capacities (Chen, Zhou, Zhou & Chen ; Zhang & Wu ). Rehmannia glutinosa root products enhance the growth and immune parameters of Cyprinus carpio (Wang, Meng, Lu, Wu, Luo, Yan, Li, Kong & Nie ; Wang, Chan, Rêgo, Chong, Ai, Falcão, Rádis‐Baptista & Lee ). The seeds of Achyranthes aspera enhance immune responses in Catla catla (Chakrabarti, Srivastava, Verma & Sharma ).…”

Section: β‐Glucansmentioning

confidence: 99%

Application of immunostimulants in aquaculture: current knowledge and future perspectives

et al. 2016

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Similar to other industries, aquaculture constantly requires new techniques to increase production yields. Modern technologies and different scientific fields, such as biotechnology and microbiology, provide important tools that could lead to a higher quality and a greater quantity of products. New feeding practices in farming typically play an important role in aquaculture, and the addition of various additives to a balanced feed formula to achieve better growth is a common practice of many fish and shrimp feed manufacturers and farmers. As 'biofriendly agents', immunostimulants, such as biological factors, probiotics and vitamins, can be introduced into the culture environment to control and kill pathogenic bacteria, as well as to promote growth of the cultured organisms. In addition, immunostimulants are non-pathogenic and nontoxic and do not produce undesirable side effects when administered to aquatic organisms. In this review, we summarize previous studies performed with both traditional immunostimulants and the most promising new generation of immunostimulants, such as polysaccharides, nutrients, oligosaccharides, herbs, microorganisms, prebiotics and different biological factors. This review primarily focuses on their protective efficacies and on what is known concerning their effects on the immune systems of aquatic organisms when delivered in vivo.

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“…The activity of Ctn against trypanosomatid protozoa was also experimentally evidenced in vitro [2]. The Ctn was structurally dissected in two pharmacophore fragments, the 14-residue, N-terminal Ctn [1][2][3][4][5][6][7][8][9][10][11][12][13][14] and the 20-residue, C-terminal Ctn [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34], retrieving the antimicrobial and antitumor activities of the full-length peptides [3]. In comparison to the fulllength Ctn, the N-and C-terminal fragments showed distinct and selective activity against Gram-negative bacteria and some lines of tumor cells.…”

Section: A9mentioning

confidence: 99%

“…Ctn [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] was also effective in vitro as an antiviral agent against infection myonecrosis virus (IMNV), a RNA virus that affects farming shrimps [6]. Interestingly, repetitive in tandem encrypted vipericidin peptides display selective structure-related cytotoxicity to zebrafish and mammary gland tumor cells [7]. Altogether, the broad spectrum of activities of these venom-and cathelicidin-derived peptide compounds, the antiproliferative activity is amenable to be expanded against selected targets and the core peptide structure tamed to be developed in innovative pharmaceutical products.…”

Section: A9mentioning

confidence: 99%

Abstracts from The International Conference on Medicinal Plants and Bioeconomy & the 1st Sino-CPLP Symposium on Natural Products and Biodiversity Resources

2018

Chin Med

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Rhodamine B-conjugated encrypted vipericidin nonapeptide is a potent toxin to zebrafish and associated with in vitro cytotoxicity

Cited by 26 publications

References 48 publications

Genomewide Analysis of the Antimicrobial Peptides in Python bivittatus and Characterization of Cathelicidins with Potent Antimicrobial Activity and Low Cytotoxicity

Genomewide Analysis of the Antimicrobial Peptides in Python bivittatus and Characterization of Cathelicidins with Potent Antimicrobial Activity and Low Cytotoxicity

Application of immunostimulants in aquaculture: current knowledge and future perspectives

Abstracts from The International Conference on Medicinal Plants and Bioeconomy & the 1st Sino-CPLP Symposium on Natural Products and Biodiversity Resources

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