Rhodanine derivative LJ001 inhibits TGEV and PDCoV replication in vitro

Zhang, Yunfei; Lu, Xia; Yuan, Yixin; Li, Qianqian; Han, Li; Yang, Gangyi; Hu, Hui

doi:10.1016/j.virusres.2020.198167

Cited by 16 publications

(13 citation statements)

References 22 publications

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“…Lithium chloride and diammonium glycyrrhizinate (DG) were reported to inhibit PDCoV replication at the early stage in LLC-PK1 cells, and DG also inhibited virus attachment to the cells [ 23 ]. Rhodanine derivative LJ001 showed antiviral activity against PDCoV infection in ST cells [ 24 ]. Besides, PDCoV was shown to be susceptible to remdesivir treatment in Huh7 cells [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Antiviral effects of ergosterol peroxide in a pig model of porcine deltacoronavirus (PDCoV) infection involves modulation of apoptosis and tight junction in the small intestine

Duan

Wang

Liu

et al. 2021

Vet Res

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Porcine deltacoronavirus (PDCoV) is a newly discovered swine enteropathogenic coronavirus with worldwide distribution. However, efficient strategies to prevent or treat the infection remain elusive. Our in vitro study revealed that ergosterol peroxide (EP) from the mushroom Cryptoporus volvatus has efficient anti-PDCoV properties. The aim of this study is to evaluate the potential of EP as a treatment for PDCoV in vivo and elucidate the possible mechanisms. Seven-day-old piglets were infected with PDCoV by oral administration in the presence or absence of EP. Piglets infected with PDCoV were most affected, whereas administration of EP reduced diarrhea incidence, alleviated intestinal lesion, and decreased viral load in feces and tissues. EP reduced PDCoV-induced apoptosis and enhanced tight junction protein expressions in the small intestine, maintaining the integrity of the intestinal barrier. EP showed immunomodulatory effect by suppressing PDCoV-induced pro-inflammatory cytokines and the activation of IκBα and NF-κB p65, and upregulating IFN-I expression. Knockdown of p38 inhibited PDCoV replication and alleviated PDCoV-induced apoptosis, implying that EP inhibited PDCoV replication and alleviated PDCoV-induced apoptosis via p38/MAPK signaling pathway. Collectively, ergosterol peroxide can protect piglets from PDCoV, revealing the potential of EP for development as a promising strategy for treating and controlling the infection of PDCoV.

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Section: Discussionmentioning

confidence: 99%

Antiviral effects of ergosterol peroxide in a pig model of porcine deltacoronavirus (PDCoV) infection involves modulation of apoptosis and tight junction in the small intestine

Duan

Wang

Liu

et al. 2021

Vet Res

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“…Rhodanine derivative LJ001 possesses effective inhibitory ability at the replication stage of PDCoV life cycle in ST cells. It restricts the synthesis of viral RNA and protein, and reduces viral production ( 88 ). 25-hydroxycholesterol (25HC), the product of cholesterol oxidation by the enzyme CH25H, inhibits PDCoV infection by blocking PDCoV entry ( 70 ).…”

Section: Current Anti-pdcov Strategiesmentioning

confidence: 99%

An Updated Review of Porcine Deltacoronavirus in Terms of Prevalence, Pathogenicity, Pathogenesis and Antiviral Strategy

Duan

2022

Front. Vet. Sci.

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The recent experience with SARS-COV-2 has raised our alarm about the cross-species transmissibility of coronaviruses and the emergence of new coronaviruses. Knowledge of this family of viruses needs to be constantly updated. Porcine deltacoronavirus (PDCoV), a newly emerging member of the genus Deltacoronavirus in the family Coronaviridae, is a swine enteropathogen that causes diarrhea in pigs and may lead to death in severe cases. Since PDCoV diarrhea first broke out in the United States in early 2014, PDCoV has been detected in many countries, such as South Korea, Japan and China. More importantly, PDCoV can also infect species other than pigs, and infections have even been reported in children, highlighting its potential for cross-species transmission. A thorough and systematic knowledge of the epidemiology and pathogenesis of PDCoV will not only help us control PDCoV infection, but also enable us to discover the common cellular pathways and key factors of coronaviruses. In this review, we summarize the current knowledge on the prevalence, pathogenicity and infection dynamics, pathogenesis and immune evasion strategies of PDCoV. The existing anti-PDCoV strategies and corresponding mechanisms of PDCoV infection are also introduced, aiming to provide suggestions for the prevention and treatment of PDCoV and zoonotic diseases.

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“…For example, lithium chloride and diammonium glycyrrhizinate could inhibit PDCoV replication in LLC-PK cells in a dose-dependent manner ( Zhai et al, 2019 ). In addition, Zhang explored the inhibitory effect of LJ001 on PDCoV in three ways ( Zhang et al, 2020 ). He found that the antiviral effect of pretreatment was not significant, which may be because the receptor sites available for virus attachment on the cell membrane are altered after the cells are pretreated with the drug, which affects the fusion of virus and cells ( Zhang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, Zhang explored the inhibitory effect of LJ001 on PDCoV in three ways ( Zhang et al, 2020 ). He found that the antiviral effect of pretreatment was not significant, which may be because the receptor sites available for virus attachment on the cell membrane are altered after the cells are pretreated with the drug, which affects the fusion of virus and cells ( Zhang et al, 2020 ). Studies on the inhibition of viral replication by selenium have been reported, for example, HIV, Coxsackie virus, influenza virus, and PCV ( Beck et al, 1994 ; Schrauzer and Sacher, 1994 ; Nelson et al, 2001 ; Jaspers et al, 2007 ; Qian et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Antiviral Effect of Selenomethionine on Porcine Deltacoronavirus in Pig Kidney Epithelial Cells

Ren

Jia

et al. 2022

Front. Microbiol.

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Porcine deltacoronavirus (PDCoV) is an emerging porcine intestinal coronavirus in recent years, which mainly causes different degrees of vomiting and diarrhea in piglets and has caused great harm to the swine husbandry worldwide since its report. Selenium is an essential trace element for organisms and has been demonstrated to have antiviral effects. In this study, pig kidney epithelial (LLC-PK) cells were used to study the antiviral activity of selenomethionine (Se-Met) (2, 4, 8, and 16 μM) against PDCoV by detecting the replication of the virus, the expression of the mitochondrial antiviral signal protein (MAVS) protein, and the phosphorylation of interferon regulatory factor-3 (IRF-3), IFN-α, and IFN-β, and the changes in glutathione content, glutathione peroxidase, superoxide dismutase activity, and hydrogen peroxide content in the cells. The results showed that Se-Met at higher than physiological concentrations (16 μM) could significantly inhibit the replication of PDCoV in LLC-PK cells and enhance the expression of MAVS protein and the phosphorylation of IRF-3. In addition, Se-Met also improved the intracellular production of IFNα/β and antioxidant capacity with increasing doses. These data suggest that the availability of selenium through selenomethionine supports the antiviral response in porcine kidney cells, and the specific mechanism is attributed to the improved cellular antioxidant capacity and activation of the MAVS pathway by Se-Met.

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Rhodanine derivative LJ001 inhibits TGEV and PDCoV replication in vitro

Abstract: Highlights LJ001 have the antiviral activity against TGEV or PDCoV infection in vitro. LJ001 inhibits TGEV or PDCoV infection at the replication stages of viral life cycle. LJ001 may serve as a new candidate for treatment of swine enteric coronavirus infection.

Cited by 16 publications

References 22 publications

Antiviral effects of ergosterol peroxide in a pig model of porcine deltacoronavirus (PDCoV) infection involves modulation of apoptosis and tight junction in the small intestine

Antiviral effects of ergosterol peroxide in a pig model of porcine deltacoronavirus (PDCoV) infection involves modulation of apoptosis and tight junction in the small intestine

An Updated Review of Porcine Deltacoronavirus in Terms of Prevalence, Pathogenicity, Pathogenesis and Antiviral Strategy

Antiviral Effect of Selenomethionine on Porcine Deltacoronavirus in Pig Kidney Epithelial Cells

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