Rhodnius prolixus supergene families of enzymes potentially associated with insecticide resistance

Schama, Renata; Pedrini, Nicolás; Juárez, M. Patricia; Nelson, David R.; Torres, André; Valle, Denise; Mesquita, Rafael D.

doi:10.1016/j.ibmb.2015.06.005

Cited by 65 publications

(83 citation statements)

References 103 publications

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“…Black star indicates the T. notatus GST PUT down-regulated in mirids feeding on ir AOC plants. Genes from A. pisum ( black ) were taken from [53] and genes from R. prolixus ( green ) from [54]. T. notatus contigs are in red …”

Section: Resultsmentioning

confidence: 99%

“…Redundant sequences were then merged to obtain a non-redundant list of putatively unique transcripts (PUTs), although it should be noted that we cannot exclude that some PUTs might be allelic variants of the same gene. PUTs were then translated into proteins and for each gene family, an amino acid alignment was constructed in MEGA v6 using genomically annotated sequences from other insect species: A. pisum [53] and Rhodnius prolixus P450s and GSTs [54], Bombyx mori [19] and D. melanogaster UGTs [55]. The alignments were manually edited and used to construct ML trees in MEGA v6 using 100 bootstrap pseudo-replicates.…”

Section: Methodsmentioning

confidence: 99%

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Transcriptome profiling reveals differential gene expression of detoxification enzymes in a hemimetabolous tobacco pest after feeding on jasmonate-silenced Nicotiana attenuata plants

2016

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BackgroundThe evolutionary arms race between plants and insects has driven the co-evolution of sophisticated defense mechanisms used by plants to deter herbivores and equally sophisticated strategies that enable phytophagous insects to rapidly detoxify the plant’s defense metabolites. In this study, we identify the genetic determinants that enable the mirid, Tupiocoris notatus, to feed on its well-defended host plant, Nicotiana attenuata, an outstanding model for plant-insect interaction studies.ResultsWe used an RNAseq approach to evaluate the global gene expression of T. notatus after feeding on a transgenic N. attenuata line which does not accumulate jasmonic acid (JA) after herbivory, and consequently accumulates very low levels of defense metabolites. Using Illumina sequencing, we generated a de novo assembled transcriptome which resulted in 63,062 contigs (putative transcript isoforms) contained in 42,610 isotigs (putative identified genes). Differential expression analysis based on RSEM-estimated transcript abundances identified 82 differentially expressed (DE) transcripts between T. notatus fed on wild-type and the defenseless plants. The same analysis conducted with Corset-estimated transcript abundances identified 59 DE clusters containing 85 transcripts. In both analyses, a larger number of DE transcripts were found down-regulated in mirids feeding on JA-silenced plants (around 70%). Among these down-regulated transcripts we identified seven transcripts possibly involved in the detoxification of N. attenuata defense metabolite, specifically, one glutathione-S-transferase (GST), one UDP-glucosyltransferase (UGT), five cytochrome P450 (P450s), and six serine proteases. Real-time quantitative PCR confirmed the down-regulation for six transcripts (encoding GST, UGT and four P450s) and revealed that their expression was only slightly decreased in mirids feeding on another N. attenuata transgenic line specifically silenced in the accumulation of diterpene glycosides, one of the many classes of JA-mediated defenses in N. attenuata.ConclusionsThe results provide a transcriptional overview of the changes in a specialist hemimetabolous insect associated with feeding on host plants depleted in chemical defenses. Overall, the analysis reveals that T. notatus responses to host plant defenses are narrow and engages P450 detoxification pathways. It further identifies candidate genes which can be tested in future experiments to understand their role in shaping the T. notatus-N. attenuata interaction.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3348-0) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Transcriptome profiling reveals differential gene expression of detoxification enzymes in a hemimetabolous tobacco pest after feeding on jasmonate-silenced Nicotiana attenuata plants

2016

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“…In the R. prolixus genome, 33 IRs have been identified. Other sensory-related genes annotated for this species include gustatory receptors (GRs = 28); odorant binding proteins (OBPs = 27); chemosensory proteins (CSPs = 19); sensory neuron membrane proteins (SNMPs = 1); secreted esterases belonging to the detoxification/pheromone hormone processing class (10); CYP4 clade members (22); transient receptor potential channels (TRPs = 9); and pickpocket receptors (PPKs = 6)1821.…”

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confidence: 99%

The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features

Latorre-Estivalis

Robertson

Walden

et al. 2017

Sci Rep

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The triatomine bug Rhodnius prolixus is a main vector of Chagas disease, which affects several million people, mostly in Latin-America. Host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. The molecular bases of these sensory processes are largely unknown. The expression levels of genes transcribed in antennae were compared between 5th instar larvae, and female and male adults by means of RNA-Seq. The antennae of R. prolixus showed increased expression of several chemosensory-related genes in imaginal bugs, while both sexes had similar expression patterns for most target genes. Few cases suggest involvement of target genes in sexually dimorphic functions. Most odorant and ionotropic receptor genes seemed to be expressed in all libraries. OBPs and CSPs showed very high expression levels. Other sensory-related genes such as TRPs, PPKs and mechanoreceptors had consistent levels of expression in all libraries. Our study characterises most of the sensory gene repertoire of these insects, opening an avenue for functional genetics studies. The increase in expression of chemosensory genes suggests an enhanced role in adult bugs. This knowledge allows developing new behaviour interfering strategies, increasing the options for translational research in the vector control field.

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“…This could reflect the specificity of each isoenzyme of this large family involved in the resistance mechanism to each particular insecticide type (Schama et al 2016). Therefore, the specific enzyme family we evaluated by this test was not likely responsible for the resistance observed.…”

Section: Discussionmentioning

confidence: 99%

Toxicological, Enzymatic, and Molecular Assessment of the Insecticide Susceptibility Profile ofTriatoma infestans(Hemiptera: Reduviidae, Triatominae) Populations From Rural Communities of Santa Cruz, Bolivia

Santo-Orihuela¹,

Vassena

Carvajal³

et al. 2016

J Med Entomol

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A wide range of insecticide resistance profiles has been reported across Bolivian domestic and sylvatic populations of Triatoma infestans (Klug, 1834) (Hemiptera, Reduviidae), including some with levels proven to be a threat for vector control. In this work, the insecticide profile of domestic T. infestans was studied with standardized toxicological bioassays, in an area that has not undergone consistent vector control. F1 first-instarnymphs hatched in laboratory from bugs captured in three communities from the Santa Cruz Department were evaluated with different insecticides. Moreover, the enzymatic activity of esterases and cytochrome P450 monooxygenases was measured in individual insects to evaluate the possible mechanism of metabolic resistance to pyrethroids. In addition, the DNA sequence of sodium channel gene (kdr) was screened for two point mutations associated with pyrethroid resistance previously reported in T. infestans. All populations showed reduced susceptibility to deltamethrin and α-cypermethrin, albeit the RR50 values varied significantly among them. Increased P450 monooxygenases and permethrate esterases suggest the contribution, as detoxifying mechanisms, to the observed resistance to deltamethrin in all studied populations. No individuals presented either mutation associated to resistance in the kdr gene. The level of susceptibility to α-cypermethrin, the insecticide used by the local vector control program, falls within an acceptable range to continue its use in these populations. However, the observed RR50 values evidence the possibility of selection for resistance to pyrethroids, especially to deltamethrin. Consequently, the use of pyrethroid insecticides should be closely monitored in these communities, which should be kept under entomological surveillance and sustained interventions.

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Rhodnius prolixus supergene families of enzymes potentially associated with insecticide resistance

Cited by 65 publications

References 103 publications

Transcriptome profiling reveals differential gene expression of detoxification enzymes in a hemimetabolous tobacco pest after feeding on jasmonate-silenced Nicotiana attenuata plants

Transcriptome profiling reveals differential gene expression of detoxification enzymes in a hemimetabolous tobacco pest after feeding on jasmonate-silenced Nicotiana attenuata plants

The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features

Toxicological, Enzymatic, and Molecular Assessment of the Insecticide Susceptibility Profile ofTriatoma infestans(Hemiptera: Reduviidae, Triatominae) Populations From Rural Communities of Santa Cruz, Bolivia

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