Rhodobacteraceae on the marine brown alga Fucus spiralis are abundant and show physiological adaptation to an epiphytic lifestyle

Dogs, Marco; Wemheuer, Bernd; Wolter, Laura; Bergen, Nils; Daniel, Rolf; Simon, Meinhard; Brinkhoff, Thorsten

doi:10.1016/j.syapm.2017.05.006

Cited by 77 publications

(63 citation statements)

References 66 publications

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“…During stressful conditions, macroalgae may need increased levels of morphogens, and many ASVs identified in the study belonged to morphogen-producing genera. Finally, some of the recovered bacteria belong to known vitamin B 12 -producing bacteria including Loktanella, Octadecabacter, and Sulfitobacter (Dogs et al, 2017), which were present across all tissues, species, and treatments in our study, hypothetically making vitamin B 12 available to their algal hosts. Vitamin B 12 (cobalamin) is an essential vitamin for fucoid algae (Fries, 1993), and these genera may contribute to algal growth during thermal stress in the high zone.…”

Section: Discussionmentioning

confidence: 84%

“…The only described Schleiferia species was isolated from a hot spring (Albuquerque et al, 2011); possibly, the Schleiferia ASVs found in this study in the high zone may have similar tolerances. Some Rhodobacteraceae are associated with thermally stressed corals (Pootakham et al, 2019), their abundance was positively correlated with the incubation temperature of F. vesiculosus cultures (Stratil et al, 2013), and they were the most abundant family found on F. spiralis (Dogs et al, 2017). Here, the abundance of Rhodobacteraceae in holdfast communities was highest in the high zone and generally correlated with levels of intertidal stress.…”

Section: Discussionmentioning

confidence: 85%

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Bacterial Communities Show Algal Host (Fucus spp.)/Zone Differentiation Across the Stress Gradient of the Intertidal Zone

et al. 2020

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 85%

Bacterial Communities Show Algal Host (Fucus spp.)/Zone Differentiation Across the Stress Gradient of the Intertidal Zone

et al. 2020

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“…Some Sulfitobacter synthesize vitamin B 12 (Dogs et al. ), which might be used by Porphyra umbilicalis in its natural habitat (Brawley et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…Sulfitobacter (Rhodobacteraceae, Alphaproteobacteria) may be one of the most significant members of the microbiome of Pum1 and is in high relative abundance across cores (≥8.26%), including >26% relative abundance in the holdfast core. Some Sulfitobacter synthesize vitamin B 12 (Dogs et al 2017), which might be used by Porphyra umbilicalis in its natural habitat (Brawley et al 2017). Four strains of Sulfitobacter increase the cell division rate of the diatom Pseudo-nitzchia multiseries PC9, and Sulfitobacter SA11 was demonstrated to synthesize the plant growth regulator IAA (Amin et al 2015).…”

Section: Discussionmentioning

confidence: 99%

A common garden experiment with Porphyra umbilicalis (Rhodophyta) evaluates methods to study spatial differences in the macroalgal microbiome

Quigley

Morrison

Mendonça

et al. 2018

Journal of Phycology

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While macroalgal microbiomes are the focus of many recent studies, there is little information about microbial spatial diversity across the thallus. Reliance on field material makes it difficult to discern whether recovered microbiomes belong to the host or its epiphytes, and technical comparisons of macroalgal samples for microbial studies are needed. Here, we use a common garden approach that avoids the problem of epiphytes, particularly at holdfasts, to examine the microbiome of Porphyra umbilicalis (strain Pum1). We used the V6 hypervariable region of the 16S rDNA with Illumina HiSeq sequencing and developed PNA clamps to block recovery of organelle V6 sequences. The common garden approach allowed us to determine differences in the microbiome at the holdfast versus blade margin. We found a notable increase in the relative abundance of Planctomycetes and Alphaproteobacteria at the holdfast, particularly of the possible symbiont Sulfitobacter sp. Nonadjacent 1.5 cm samples of blade margin had microbiomes that were not statistically different. The most abundant phylum in the overall microbiome was Proteobacteria, followed by Bacteroidetes. Because phycologists often work in remote sites, we compared three stabilization and preparation techniques and found silica gel desiccation/bead-beating and flash-freezing/lyophilization/bead-beating to be interchangeable. Core taxa (≥0.1% of sequences) across treatments were similar and accounted for ≥95% of all sequences. Finally, statistical conclusions for all comparisons were the same, regardless of which microbial community analysis tool was used: mothur or minimum entropy decomposition.

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“…4b; Table S7) may be an example of this. Some members of Rhodobacteraceae are known to play a vital role as plant symbionts, such as synthesis of B 1 and B 12 vitamins (Minich et al 2018), growth factors (Dogs et al 2017), siderophores that enhance algal growth under iron deficiency, deterrents for pathogens and metabolism of algal osmolytes (Dogs et al 2017). This observation corresponds to the lower predicted bacterial activity of vitamin biosynthesis (thiamin salvage) in grazed G. firma in relative to its ungrazed counterpart (Fig.…”

Section: Discussionmentioning

confidence: 99%

The effect of grazing on the microbiome of two commercially important agarophytes, Gracilaria firma and G. salicornia (Gracilariaceae, Rhodophyta)

et al. 2020

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Grazing, which leads to losses in biomass and drastic declines in total crop production, is one of the main concerns in seaweed aquaculture. This is also thought to affect the composition of the associated bacterial communities which are believed to play a crucial role in determining the host's health and development. Apart from morphological impairment, studying changes in the prokaryotic microbiome composition and predicted functional responses to grazing will allow us to understand the underlying effects of grazing on the seaweed host. This study is the first report of the effect of grazing on the prokaryotic microbiome of two economically important agarophytes, Gracilaria firma and Gracilaria salicornia, by high-throughput sequencing targeting the V3-V4 variable region of the 16S rRNA gene. The results indicated that for G. firma, the microbiome composition of tissues grazed by marine herbivores had significantly more agarolytic bacteria Marinagarivorans sp. and Algisphaera sp. than in ungrazed tissues. The predictive functional metagenomics for this species revealed that grazing escalated the pathway activities related to nucleotide degradation, aromatic compound degradation and aerobic sugar metabolism, while pathways associated with cell wall synthesis, aerobic respiration, vitamin biosynthesis and amino acid biosynthesis were reduced. However, for G. salicornia, the bacterial communities were not significantly affected by grazing. Nevertheless, pathways relating to anaerobic respiration and amino acid, coenzyme and vitamin B-6 biosynthesis in this species were predicted to be more active in grazed tissues, whereas the microbiome of ungrazed tissues had higher activities in bacteriochlorophyll a, fatty acid, secondary metabolite and heme biosynthesis.

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Rhodobacteraceae on the marine brown alga Fucus spiralis are abundant and show physiological adaptation to an epiphytic lifestyle

Cited by 77 publications

References 66 publications

Bacterial Communities Show Algal Host (Fucus spp.)/Zone Differentiation Across the Stress Gradient of the Intertidal Zone

Bacterial Communities Show Algal Host (Fucus spp.)/Zone Differentiation Across the Stress Gradient of the Intertidal Zone

A common garden experiment with Porphyra umbilicalis (Rhodophyta) evaluates methods to study spatial differences in the macroalgal microbiome

The effect of grazing on the microbiome of two commercially important agarophytes, Gracilaria firma and G. salicornia (Gracilariaceae, Rhodophyta)

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