Rhododenol-induced leukoderma in a mouse model mimicking Japanese skin

Abe, Yuko; Okamura, Ken; Kawaguchi, Masakazu; Hozumi, Yasukazu; Aoki, Hitomi; Kunisada, Takahiro; Itô, Shôsuke; Wakamatsu, Kazumasa; Matsunaga, Kayoko; Suzuki, Tamio

doi:10.1016/j.jdermsci.2015.10.011

Cited by 30 publications

(48 citation statements)

References 24 publications

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“…In the progressive cases, it has been proposed that the chemicals initially trigger auto‐immune responses against melanocytes These previous studies in cases of chemical leukoderma may indicate that there could be auto‐immune reactions in cases of extensive and irreversible type of RIL. Interestingly, another study with TEM using RIL model mice revealed a dilated rER in melanocytes, suggesting rER stress . Furthermore, a study using cultured melanocytes after rhododendrol treatment showed a decrease in melanosomes and a dramatic increase in lysosomes .…”

Section: Discussionmentioning

confidence: 97%

“…Ultrathin sections were examined in a JEM‐1400 transmission electron microscope (JEOL, Tokyo, Japan). In mouse experiments, the specimens were also processed for TEM routinely as previously described …”

Section: Methodsmentioning

confidence: 99%

“…To investigate the direct effects of morphological changes caused by rhododendrol, we examined specimens of the RIL mouse model ultrastructurally as previously described . All animal procedures were performed in accordance with the National Institutes of Health Guide for the Use of Laboratory Animals and were approved by the Animal Research Committee of Yamagata University . Briefly, the mice were prepared by daily application of 30% rhododendrol to the skin of hairless hk14‐SCF Tg mice .…”

Section: Methodsmentioning

confidence: 99%

“…In mouse experiments, the specimens were also processed for TEM routinely as previously described. 13…”

Section: Transmission Electron Microscopy Observationmentioning

confidence: 99%

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Leukoderma induced by rhododendrol is different from leukoderma of vitiligo in pathogenesis: A novel comparative morphological study

et al. 2018

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Background Rhododendrol (rhododenol), an inhibitor of tyrosinase activity, is used as a skin‐whitening component. Many cases of leukoderma after the application have been reported, termed rhododenol‐induced leukoderma (RIL). The aim of this study was to clarify the pathogenesis of RIL morphologically through comparison with vitiligo. Methods We examined 14 cases of RIL and 15 cases of vitiligo using routine histopathology and immunohistochemistry. Thirteen cases of RIL, six cases of vitiligo and specimens of the RIL mouse model were evaluated by electron microscopy. Results There were common findings in RIL and vitiligo at the light‐microscopic level: (a) vacuolar changes in the dermo‐epidermal junction, (b) melanophages in the papillary dermis, (c) perifollicular lymphocyte infiltration, (d) loss or decrease of basal melanin pigment and (e) decrease of melanocytes in the lesions. The ultrastructural observations showed specific findings of RIL: (a) remaining melanocytes in depigmented lesions, (b) inhomogeneous melanization in melanocytes and (c) degenerated melanosomes in melanocytes. Some of the findings were observed in a RIL mouse model. Furthermore, it is notable that cell organelles of melanocytes were intact in our RIL cases. Conclusion Morphological changes of RIL targeting melanosomes in melanocytes without degeneration of organelles reflect the reversible clinical course of most cases.

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Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…In mouse experiments, the specimens were also processed for TEM routinely as previously described. 13…”

Section: Transmission Electron Microscopy Observationmentioning

confidence: 99%

See 2 more Smart Citations

Leukoderma induced by rhododendrol is different from leukoderma of vitiligo in pathogenesis: A novel comparative morphological study

et al. 2018

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“…(a–d) Representative gross appearance of the back of hairless hk14‐ SCF transgenic mice. (a) Before the application of rhododendrol ( RD ), the skin color of the mice was darker than that of the albino mice from the same strain . (b) Almost complete depigmentation with spotty remaining pigments is induced by the application of a 30% RD solution for 14 days.…”

mentioning

confidence: 99%