Rhodoligotrophos jinshengii sp. nov., isolated from activated sludge

Abstract

A Gram-stain-negative, non-spore-forming, non-motile, ovoid, aerobic bacterial strain, designated BUT-3 T , was isolated from activated sludge from the wastewater treatment facility of a herbicidemanufacturing plant in Kunshan city, Jiangsu province, PR China. Strain BUT-3 T grew between 15 and 40 6C, with optimum growth at 30 6C. The pH range for growth was between 5.0 and 10.0 (optimum pH 7.0). The range of NaCl concentrations for growth of strain BUT-3 T was 0-7.0 % (w/v), with an optimum of 1.5-3.0 % (w/v). A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strain BUT-3 T clustered closely with Rhodoligotrophos appendicifer 120-1 T (98.32 % similarity), with a bootstrap confidence level of 100 %. The major fatty acids (.5 % of total fatty acids) were C 19 : 0 cyclo v8c, C 18 : 1 v7c, C 16 : 0 , anteiso-C 15 : 0 and iso-C 15 : 0 . Strain BUT-3 T contained ubiquinone Q-10 as the predominant respiratory quinone. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, three unidentified aminolipids (AL1-3), two unknown phospholipids (PL1, 5), four unidentified glycolipids (GL1-4) and two unknown lipids (L1, 2). The G+C content of the genomic DNA was 67.7 mol%. The DNA-DNA relatedness between BUT-3 T and R. appendicifer 120-1 T was 44.1±0.6 %. Based on the polyphasic taxonomic data, strain BUT-3 T should be classified as a representative of a novel species of the genus Rhodoligotrophos, for which the name Rhodoligotrophos jinshengii sp. nov. is proposed. The type strain is BUT-3 T (5CCTCC AB2013083 T 5KACC 17220 T ).The genus Rhodoligotrophos, established recently by Fukuda et al. (2012), belongs to the class Alphaproteobacteria, order Rhizobiales, family Rhodobiaceae. The type strain, Rhodoligotrophos appendicifer 120-1 T , was isolated from samples of freshwater from the bottom of a freshwater lake in the Antarctica. It is an oligotroph partly due to the relatively low levels of nutrients in its ecological niche. In this study, we reported a Rhodoligotrophos-like bacterial strain, designated BUT-3 T , which was isolated from activated sludge. Taxonomic analyses demonstrated that the isolate should be placed into a novel species within the genus Rhodoligotrophos.R. appendicifer 120-1 T was used as a reference strain and was purchased from the Japan Collection of Micro-organisms. Unless indicated otherwise, experiments on morphological, physiological, molecular and chemotaxonomic characteristics were performed on cells grown on 0.256LB medium (l 21 :10.0 g tryptone, 5.0 g yeast extract and 5.0 g NaCl) under aerobic conditions at 30 u C.Strain BUT-3 T was isolated from a soil sample collected from Kunshan city, Jiangsu province, PR China, using the standard dilution plating technique at 30 uC on 0.256LB medium. Unlike R. appendicifer 120-1 T , strain BUT-3 T is a copiotroph, and can grow in LB medium, TSB medium (l 21 :15.0 g tryptone, 5.0 g soy peptone and 5.0 g NaCl), R2A medium (l 21 :0.5 g tryptone, 0.5 g yeast extrac...

Kaustia mangrovi gen. nov., sp. nov. isolated from Red Sea mangrove sediments belongs to the recently proposed Parvibaculaceae family within the order Rhizobiales

“…General features and genomic and phenotypic characteristics of R1DC25 T and closely related members of the genus RhodoligotrophosStrains: 1, K. mangrovi R1DC25 T (data from this study); 2, R. appendicifer JCM 16873 T (data from [89] 90); 3, R. jinshengii BUT-3 T (data from[91]); 4, R. defluvii lm1 T (data from [85] 60). +, Positive; −, negative; na, not available.…”

Contribution to the characterization of the seed endophyte microbiome of Argania spinosa across geographical locations in Central Morocco using metagenomic approaches

“…Furthermore, many bacterial endophytes that can be found on the seeds play beneficial roles. For instance, Rhodoligotrophos have a certain capacity for adaptation and survival in different environments, as well as a potentially important ecological role in the regulation of biogeochemical cycles in different ecosystems ( Fukuda et al, 2012 ; Deng et al, 2014 ; Liu et al, 2019 ). Although Rhodoligotrophos dominated with high percentages, we also observed the presence of other genera such as Pseudomonas, Paraburkholderia, Ralstonia, Bacillus, Corynebacterium, Streptococcus, Pelomonas, Staphylococcus, Acinetobacter, and Pantoea , with significant variations depending on the studied areas.…”

Taklimakanibacter deserti gen. nov., sp. nov. and Taklimakanibacter lacteus sp. nov., isolated from desert soil

“…Differential characteristics of strains SYSU D60010 T , SYSU D60012 T and their Strains: 1, SYSU D60010 T ; 2, SYSU D60012 T ; 3, Aestuariivirga litoralis SYSU M10001 T[5]; 4, Rhodoligotrophos defluvii lm1 T[42]; 5, Rhodoligotrophos appendicifer 120-1 T[40]; 6, Rhodoligotrophos jinshengii BUT-3 T[41]. +, Positive; −, negative; w, weakly positive; nd, no data available.…”

“…General genomic features of strains SYSU D60010 T , SYSU D60012 T , and their related type strains Strains: 1, SYSU D60010 T ; 2, SYSU D60012 T ; 3, Aestuariivirga litoralis SYSU M10001 T[5]; 4, Rhodoligotrophos defluvii lm1 T[40]; 5, Rhodoligotrophos appendicifer 120-1 T[41].…”