Rhus semialata M. extract ameliorate para-phenylenediamine-induced toxicity in keratinocytes

Woo, Hyunju; Kim, Hayeon; Shin, Seoungwoo; Ryu, Dehun; Park, Deokhoon; Jung, Eunsun

doi:10.1016/j.toxrep.2020.12.020

Cited by 8 publications

(5 citation statements)

References 40 publications

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“…A number of studies have been documented the role of ROS in PPD-induced apoptosis. It has been suggested that ROS can cause collapse of the mitochondrial membrane potential and subsequent release of cytochrome c, which eventually lead to cleavage of caspase-3 and apoptotic cell death (Sang et al, 2012; Khan et al, 2012; Woo et al, 2021; Wang et al, 2022). We investigated such hypothesis in our study and observed the involvement of mitochondrial membrane potential collapse and activation of caspase cascade in PPD-induced human lymphocyte death.…”

Section: Discussionmentioning

confidence: 99%

Toxicity of para-phenylenediamine (PPD;1, 4 diaminobenzene) on isolated human lymphocytes: The key role of intracellular calcium enhancement in PPD-induced apoptosis

2023

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Para-phenylenediamine (PPD) is a derivative of benzene used as an ingredient in dyes, a photographic developing agent, and a component of engineered polymers. The carcinogenicity of PPD, which has been documented in several studies, may be related to its toxic effects on different compartments of the immune system. The main goal of this research was to evaluate the mechanism of the toxicity of PPD on human lymphocytes by exploiting the accelerated cytotoxicity mechanism screening (ACMS) technique. Lymphocytes were isolated from the blood of healthy persons using a Ficoll-Paque PLUS standard method. Assessment of cell viability was carried out 12 h following treatment of human lymphocytes with 0.25–1 mM PPD. For determination of cellular parameters, isolated human lymphocytes were incubated with 1/2 the IC50 (0.4 mM), the IC50 (0.8 mM), and twice the IC50 (1.6 mM) for 2, 4, and 6 h. Half maximal inhibitory concentration (IC50) is the concentration that reduces cell viability approximately 50% following treatment. The results of this study demonstrated that PPD-associated apoptosis in human lymphocytes was mainly through the enhancement of intracellular calcium, oxidative stress, and following adverse effect on lymphocyte organelles (like mitochondria and lysosomes). Lipid peroxidation, activation of caspase-3, and stimulation of cytokines (IL2, interferon-gamma (IFN-γ), and TNF-alpha) production were also observed in PPD-treated lymphocytes. Considering the results of this study, we can suggest an association between PPD carcinogenicity and its toxic effects on different compartments of the immune system.

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Section: Discussionmentioning

confidence: 99%

Toxicity of para-phenylenediamine (PPD;1, 4 diaminobenzene) on isolated human lymphocytes: The key role of intracellular calcium enhancement in PPD-induced apoptosis

2023

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“… 99 Importantly, another possible explanation for the reduced incidence of some cancers in atopic individuals is avoidance behavior, which is crucial to preventing episodes of hypersensitivity and certainly reduces the exposure of atopic individuals to allergens and irritants, some of which are considered mutagens. 100 …”

Section: Adaptive Immunity In Cancermentioning

confidence: 99%

Distinct T helper cell-mediated antitumor immunity: T helper 2 cells in focus

Silva¹,

Lopes²,

Correa³

2023

Cancer Pathogenesis and Therapy

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“…To analyze the changes induced by CGA in the mitochondrial membrane potential, we used staining with DiOC6, a lipophilic fluorescent dye that is sensitive to mitochondria in living cells at low concentrations [14] . Fig.…”

Section: Data Descriptionmentioning

confidence: 99%

Data on the cytotoxicity of chlorogenic acid in 3D cultures of HT-29 cells

Vélez,

Pedroza-Díaz,

Santa-González

2023

Data in Brief

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Rhus semialata M. extract ameliorate para-phenylenediamine-induced toxicity in keratinocytes

Cited by 8 publications

References 40 publications

Toxicity of para-phenylenediamine (PPD;1, 4 diaminobenzene) on isolated human lymphocytes: The key role of intracellular calcium enhancement in PPD-induced apoptosis

Toxicity of para-phenylenediamine (PPD;1, 4 diaminobenzene) on isolated human lymphocytes: The key role of intracellular calcium enhancement in PPD-induced apoptosis

Distinct T helper cell-mediated antitumor immunity: T helper 2 cells in focus

Data on the cytotoxicity of chlorogenic acid in 3D cultures of HT-29 cells

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