Rhythmic control of AANAT translation by hnRNP Q in circadian melatonin production

Kim, Tae-Don; Woo, Kyung-Chul; Cho, Sungchan; Ha, Dae-Cheong; Jang, Sung Key; Kim, Kyong‐Tai

doi:10.1101/gad.1519507

Cited by 91 publications

(97 citation statements)

References 52 publications

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“…Despite the fact that HCV and FMDV viral RNA are never found in the nuclei of infected cells, complexes formed on IRES elements share components with the splicing machinery. Involvement of splicing factors and hnRNPs in internal initiation has been reported for SRp20 (49), SF2/ASF (50), PTB (hnRNP I) (37,43), hnRNP A1 (51), or hnRNP Q (52). Most of the identified ITAFs are very abundant and shuttle between the nucleus and the cytoplasm.…”

Section: Discussionmentioning

confidence: 92%

A novel role for Gemin5 in mRNA translation

Pacheco

Quinto

Ramajo

et al. 2008

Nucleic Acids Research

126

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In eukaryotic cells translation initiation occurs through two alternative mechanisms, a cap-dependent operating in the majority of mRNAs, and a 5′-end-independent driven by internal ribosome entry site (IRES) elements, specific for a subset of mRNAs. IRES elements recruit the translation machinery to an internal position in the mRNA through a mechanism involving the IRES structure and several trans-acting factors. Here, we identified Gemin5 protein bound to the foot-and-mouth disease virus (FMDV) and hepatitis C virus (HCV) IRES using two independent approaches, riboproteomic analysis and immunoprecipitation of photocroslinked factors. Functional analysis performed in Gemin5 shRNA-depleted cells, or in in vitro translation reactions, revealed an unanticipated role of Gemin5 in translation control as a down-regulator of cap-dependent and IRES-driven translation initiation. Consistent with this, pull-down assays showed that Gemin5 forms part of two distinct complexes, a specific IRES-ribonucleoprotein complex and an IRES-independent protein complex containing eIF4E. Thus, beyond its role in snRNPs biogenesis, Gemin5 also functions as a modulator of translation activity.

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Section: Discussionmentioning

confidence: 92%

A novel role for Gemin5 in mRNA translation

Pacheco

Quinto

Ramajo

et al. 2008

Nucleic Acids Research

126

View full text Add to dashboard Cite

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“…Fractionation of NIH3T3 cells into cytosolic extracts was performed as described (30). Immunoblot analyses were performed with monoclonal anti-14-3-3ξ (Santa Cruz), polyclonal antibody against hnRNP Q (Sigma-Aldrich).…”

Section: Methodsmentioning

confidence: 99%

hnRNP Q mediates a phase-dependent translation-coupled mRNA decay of mouse Period3

Kim

Kwak

Kim

et al. 2011

Nucleic Acids Research

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Daily mRNA oscillations of circadian clock genes largely depend on transcriptional regulation. However, several lines of evidence highlight the critical role of post-transcriptional regulation in the oscillations of circadian mRNA oscillations. Clearly, variations in the mRNA decay rate lead to changes in the cycling profiles. However, the mechanisms controlling the mRNA stability of clock genes are not fully understood. Here we demonstrate that the turnover rate of mouse Period3 (mPer3) mRNA is dramatically changed in a circadian phase-dependent manner. Furthermore, the circadian regulation of mPer3 mRNA stability requires the cooperative function of 5′- and 3′-untranslated regions (UTRs). Heterogeneous nuclear ribonucleoprotein Q (hnRNP Q) binds to both 5′- and 3′-UTR and triggers enhancement of translation and acceleration of mRNA decay. We propose the phase-dependent translation coupled mRNA decay mediated by hnRNP Q as a new regulatory mechanism of the rhythmically regulated decay of mPer3 mRNA.

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“…This results in elevation of adenosine monophosphate (5′-AMP) and increases the synthesis of the rate-limiting enzyme of melatonin synthesis, the arylalkylamine-N-acetyltransferase (Aanat) (reviewed in [32]). There is evidence that the synthesis of Aanat mRNA is regulated by the circadian clock by translational mechanisms [38]. Direct interaction of clock components with the E-box present in the promoter of the Aanat gene appears to modulate its expression also transcriptionally in a time-dependent fashion [29].…”

Section: Neurotransmitters Sleep and Circadian Rhythmsmentioning

confidence: 99%

Circadian rhythms and sleep—the metabolic connection

Albrecht

2011

Pflugers Arch - Eur J Physiol

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The circadian system coordinates mammalian physiology and behavior with the environmental light-dark cycle. It allocates sleep to the inactivity phase using various mechanisms involving neurotransmitters, nuclear receptors, and protein kinases. These pathways are related to metabolism, indicating that the circadian system and sleep are connected via metabolic parameters. This suggests that organs other than the brain may "sleep." A hypothetic view on this aspect is presented providing a different perspective on sleep regulation.

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Rhythmic control of AANAT translation by hnRNP Q in circadian melatonin production

Cited by 91 publications

References 52 publications

A novel role for Gemin5 in mRNA translation

A novel role for Gemin5 in mRNA translation

hnRNP Q mediates a phase-dependent translation-coupled mRNA decay of mouse Period3

Circadian rhythms and sleep—the metabolic connection

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