Abstract-A comparative study of the application of Gaussian Mixture Model (GMM) and Radial Basis Function (RBF) in biometric recognition of voice has been carried out and presented. The application of machine learning techniques to biometric authentication and recognition problems has gained a widespread acceptance. In this research, a GMM model was trained, using Expectation Maximization (EM) algorithm, on a dataset containing 10 classes of vowels and the model was used to predict the appropriate classes using a validation dataset. For experimental validity, the model was compared to the performance of two different versions of RBF model using the same learning and validation datasets. The results showed very close recognition accuracy between the GMM and the standard RBF model, but with GMM performing better than the standard RBF by less than 1% and the two models outperformed similar models reported in literature. The DTREG version of RBF outperformed the other two models by producing 94.8% recognition accuracy. In terms of recognition time, the standard RBF was found to be the fastest among the three models.