Rib and vertebral deformities in rainbow trout (Oncorhynchus mykiss) explained by a dominant-mutation mechanism

Gislason, Hannes; Karstensen, Helena; Christiansen, Debes H.; Hjelde, Kirsti; Helland, Ståle J.; Bæverfjord, Grete

doi:10.1016/j.aquaculture.2010.09.016

Cited by 16 publications

(10 citation statements)

References 19 publications

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“…In both groups, we observed that the vertebral arches were not fully ossified, but no significant differences were observed in their mineralization status (Table 3). Furthermore, no obvious presence of ectopic cartilage or remodelling of the notochord was observed in either deformed or normal fish, as previously reported in severe compression type deformities in other salmonids [24,96,108].…”

Section: Resultssupporting

confidence: 83%

RNA-Seq Transcriptome Analysis of Pronounced Biconcave Vertebrae: A Common Abnormality in Rainbow Trout (Oncorhynchus mykiss, Walbaum) Fed a Low-Phosphorus Diet

J¹,

MH²

2015

Next Generat Sequenc & Applic

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International audienceThe prevalence of bone deformities, particularly linked with mineral deficiency, is an important issue for fish production. Juvenile triploid rainbow trout (Oncorhynchus mykiss) were fed a low-phosphorus (P) diet for 27 weeks (60 to 630 g body mass). At study termination, 24.9% of the fish fed the low-P diet displayed homogeneous biconcave vertebrae (deformed vertebrae phenotype), while 5.5% displayed normal vertebral phenotypes for the entire experiment. The aim of our study was to characterize the deformed phenotype and identify the putative genes involved in the appearance of P deficiency-induced deformities. Both P status and biomechanical measurements showed that deformed vertebrae were significantly less mineralized (55.0 ± 0.4 and 59.4 ± 0.5,% ash DM, for deformed and normal vertebrae, respectively) resulting in a lower stiffness (80.3 ± 9.0 and 140.2 ± 6.3 N/mm, for deformed and normal phenotypes, respectively). The bone profiles based on μCT observations showed no difference in the osteoclastic resorption while no difference in matrix production was observed between deformed (total bone area 5442.0 ± 110.1 μm2) and normal vertebrae (total bone area 5931.2 ± 249.8 μm2) in this study. Consequently, the lower P content rather results from a reduced degree of mineralization in the deformed phenotype. Finally, we quantified differential gene expression between deformed vertebrae (pronounced biconcave) and normal phenotype by employing deep RNA-sequencing and mapping against a reference bone transcriptome for rainbow trout. In total, 1289 genes were differentially expressed. Among them, in deformed fish we observed that BGLAP, MGP and NOG, an inhibitor of BMP signalling pathway, were up-regulated while COL11a1 was down-regulated. These genes are central actors involved in the reduced degree of mineralization triggering vertebral deformities. These results will further the understanding of P deficiency-induced deformities; hence providing new tools for improved P management in production setting

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Section: Resultssupporting

confidence: 83%

RNA-Seq Transcriptome Analysis of Pronounced Biconcave Vertebrae: A Common Abnormality in Rainbow Trout (Oncorhynchus mykiss, Walbaum) Fed a Low-Phosphorus Diet

J¹,

MH²

2015

Next Generat Sequenc & Applic

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“…This difference in the percentage of incompletely hatched embryos could have several causes, e.g., the functionality of the hatching enzyme, the biochemical characteristics of the vitelline envelope or embryo movement (Kunz, 2004). Underlying these causes, variations in degree of heterozygosity or specific mutations could be involved (Boglione et al, 2014;Danzmann et al, 1986;Gislason et al, 2010). In particular, the incomplete hatching of embryos, with the head and yolk sac remaining encapsulated within the vitelline envelope, has been depicted as a consequence of acid stress by Sayer et al (1993), but here a stable pH was maintained throughout the experiment.…”

Section: Discussionmentioning

confidence: 99%

Thermal effects in rainbow trout (Oncorhynchus mykiss) F1 embryos (farmed female × wild thermal‐resistant male)

Crichigno

Orellana

Larraza

et al. 2021

Journal of Fish Biology

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The aim of this work was to investigate the response of rainbow trout embryos (Oncorhynchus mykiss) (i.e., survival, size at hatching, time to hatching, malformations) to four incubation temperatures (5.8, 8.9, 14.0 and 16.8 C), taking into account the origin of the male parental genome and comparing pure farmed and F1 embryos (farmed female × wild thermal-resistant male). Several consequences of thermal stress were observed: lower accumulated thermal units (ATU) at hatching at high temperatures, and lower survival, shorter hatched free embryos and less-consumed yolk sac at extreme temperatures. The effect of the thermal-adapted male parental genome was shown only in the lower percentage of incompletely hatched free embryos in the F1 families. It appears that to obtain greater modification of thermal performance during early development, the adapted genome of the wild thermalresistant population has to be included through maternal inheritance, thus producing a stabilized strain selected for domesticity, growth and thermal adaptation.

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“…Many of them have demonstrated that alterations on biotic and abiotic, physiological, xenobiotic, nutritional, and rearing factors can be responsible for the development of fish deformities at an early stage (Boglione et al, 2001;Bardon et al, 2009;Boglione and Costa, 2011;Prestinicola et al, 2013) or during their on-growing period (I. Lee-Montero, personal communication). Additionally, genetic factors are also possibly responsible for the prevalence of different skeletal deformities in different species: Atlantic salmon (Salmo salar; Gjerde, 2005), rainbow trout (Oncorhynchus mykiss; Kause et al, 2005;Gislason et al, 2010), Atlantic cod (Gadus morhua; Kolstad et al, 2006) and European seabass (Dicentrarchus labrax; Bardon et al, 2009, Karahan et al, 2013. However, in gilthead seabream, only a few studies have proposed a genetic origin for some types of deformities (Afonso et al, 2000;Astorga et al, 2004;I.…”

Section: Discussionmentioning

confidence: 99%

Inheritance of skeletal deformities in gilthead seabream (Sparus aurata) – lack of operculum, lordosis, vertebral fusion and LSK complex1

Negrín-Báez

Navarro

Lee-Montero

et al. 2015

Journal of Animal Science

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Morphological abnormalities in farmed gilthead seabream (Sparus aurata) are a major problem as it entails significant economic losses. In this study, 3 large scale experiments under different conditions of spawning, offspring handling and breeders phenotype were performed to analyze the inheritance of 4 types of deformities in this species: lack of operculum, lordosis, vertebral fusion, which are 3 of the most important skeletal deformities, and LSK, which is a consecutive repetition of lordosis/scoliosis/kyphosis. In Exp. [1] (mass spawning and fingerling sorting), 900 fish were analyzed at 509 d post-hatching: 846 fish that had been on-grown in a farm and 54 LSK-deformed fish that had been reared separately after being selected during the fingerling sorting process. A total of 89 families were represented. A statistically significant association between 5 of these families (from 6 breeders) and LSK-deformed fish was found. In Exp. [2] (mass spawning and no fingerling sorting), 810 fish were analyzed at 2 ages: 179 and 689 d post-hatching. Significant relationships between 2 of the breeders and 2 of the families with the lack of operculum prevalence of their descendants were found at 689 d but not at 179 d. Heritabilities: 0.09 ± 0.09 at 179 d and 0.17 ± 0.08 at 689 d. Column deformities prevalence was low and no association with family was observed. Family relationships were determined by microsatellites multiplex PCR in both experiments. In Exp. [3] (designed mating), sires suffering from lordosis or lack of operculum or vertebral fusion deformities were mated with non-deformed dams and a mass-spawning mating was considered as a control. After analyzing 11,503 offspring at 159 d post-hatching, a significant relationship between each deformity prevalence and the mating of breeders suffering from the same deformity was observed. In addition, a significant prevalence of lack of operculum in offspring from lordotic matings was observed. Heritabilities ranged from 0.34 to 0.46 for the 3 deformities. The results of the present study suggest that these deformities have a genetic origin. They also suggest that the sorting process is not recommended and that producers should consider these deformities in genetic breeding programs to significantly improve their fish morphological quality and to minimize farmed fish deformities incidence.

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Rib and vertebral deformities in rainbow trout (Oncorhynchus mykiss) explained by a dominant-mutation mechanism

Cited by 16 publications

References 19 publications

RNA-Seq Transcriptome Analysis of Pronounced Biconcave Vertebrae: A Common Abnormality in Rainbow Trout (Oncorhynchus mykiss, Walbaum) Fed a Low-Phosphorus Diet

RNA-Seq Transcriptome Analysis of Pronounced Biconcave Vertebrae: A Common Abnormality in Rainbow Trout (Oncorhynchus mykiss, Walbaum) Fed a Low-Phosphorus Diet

Thermal effects in rainbow trout (Oncorhynchus mykiss) F1 embryos (farmed female × wild thermal‐resistant male)

Inheritance of skeletal deformities in gilthead seabream (Sparus aurata) – lack of operculum, lordosis, vertebral fusion and LSK complex1

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