Ribgrass Mosaic Tobamovirus Occurred on Chinese Cabbage in Korea

Abstract

A tobamovirus, Ribgrass mosaic virus (RMV), was identified newly from chinese cabbage (Brassica campestris L. pekinensis) in Korea. Virus disease incidence of RMV on chinese cabbage was 37.9% in alpine area on August in 1993. RMV induced the symptoms of necrotic ring spots, necrotic streak on midrib and malformation. RMV, Ca1 and Ca3 isolate, could infect 35 species out of 45 plants including Chenopodium amaranticolor. Physical properties of RMV Ca1 isolate were very stable as 10 −8 over for dilution end point, 95 o C for temperature inactivation point and 18 weeks for longevity in vitro. RMV had the soil transmission rate of 75.0% for the chinese cabbages, 'Chunhawang' and 'Seoul' cultivars. The purified virions of RMV had the typical ultraviolet absorption spectrum of maximum at 260 nm and minimum at 247 nm. RMV of Ca1 isolate was related serologically with antisera of Tobacco mosaic virus (TMV)-Cym, TMV-O and Pepper mottle virus, but not related with antiserum of Odontoglossum ring spot virus. coat protein gene of RMV-Ca1, sized 473 nucleotides, encoded 158 amino acid residues. Nucleotide identity of RMV-Ca1 CP gene was 96.4% with RMV-Shanghai (GenBank accession No. of AF185272) from China and 96.0% with RMV-Impatiens (GenBank accession No. of AM040974) from Germany. Identity of amino acids between RMV-Ca1 and the two RMV isolates was 96.8%. Specific three primers were selected for rapid and easy genetic detection of RMV using Virion Captured (VC)/ RT-PCR method.

A hybrid simulation framework for predicting virus impacts on Chinese cabbage yields

“…Currently, virus diseases affecting Chinese cabbage include Cucumber mosaic virus, Turnip mosaic virus, and Ribgrass mosaic virus (Akram et al, 2021; Cho et al, 2002; J. S. Kim et al., 2003; J. S. Kim et al. 2010). It is well known that climate warming significantly affects the occurrence and occurrence period of agricultural virus diseases (Hu et al., 2019; Kapur, 2013; Sacks et al., 2010).…”

Brief report: genome sequence and construction of an infectious cDNA clone of Ribgrass mosaic virus from Chinese cabbage in Korea

“…In Korea, Chinese cabbage (Brassica rapa ssp. pekinensis) infected with RMV was reported in the fields of Pyungchang and Yeongam province, where average 45.7% of collected Chinese cabbages were infected by RMV [7]. In addition, RMV occurred as the co-infection rate of 63.4% with TuMV, of which exacerbated symptoms and growth of Chinese cabbage, although the two viruses are quite different in ecological characteristics of transmission manners, physical properties, and morphology of virus particles [8].…”