Ribodemes of Blastocystis hominis isolated in Japan.

Kaneda, Yoshimasa; Horiki, Noriyuki; Cheng, Xunjia; Fujita, Yoshiyuki; Maruyama, Masataka; Tachibana, Hiroshi

doi:10.4269/ajtmh.2001.65.393

Cited by 92 publications

(78 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results are similar to some other studies performed worldwide (14,15,21,(36)(37)(38). The frequency of subtype SB83 was more than subtypes SB227 and SB340 that is similar to some other researches (5,17,20).…”

Section: Discussionsupporting

confidence: 82%

A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

Sardarian

Hajilooi

Maghsood

et al. 2012

Jundishapur J Microbiol

View full text Add to dashboard Cite

Blastocystis hominis is a common protozoan parasite in the human and many other animals intestine. The parasite pathogenicity is still controversial. A few studies have been conducted on prevalence of B. hominis subtypes in Iran. The results of present study improve our knowledge on B. hominis subtypes in Iran and therefore we can do more investigations on pathogenicity potential of these subtypes in the future. Background:Blastocystis is a common protozoan parasite in mammals, birds, amphibians, reptiles, fish, arthropods, and annelids. This parasite has some subtypes, which pathogenicity status of them still remained controversial. Some of Blastocystis subtypes are potentially pathogenic to human. Objectives: This study has identified the Blastocystis hominis subtypes and their prevalence rates in Hamadan. Materials and Methods: During two months of summer 2011, a total number of 250 human fecal samples referred for parasitology examination to Beasat Hospital and a few clinical laboratories of Hamadan city were collected. The samples were examined by direct method and formalin-ether. 41 samples exhibited positive results for B. hominis thereby were cultured in Locke-egg medium. After the growth and in order to genotype identification, B. hominis isolates were amplified by PCR, using seven pairs of sequencestagged site primers. Results: In this study, three subtypes of B. hominis consisted of one [SB83], two [SB340] and three [SB227] were identified. The most dominant genotype was SB83 with 56.1 % frequency. The prevalence rate of genotype SB227 and SB340 were 22 % and 7.3 %, respectively. Coexistence of genotypes SB83 and SB227 was detected in 14.6 % of positive cases. Conclusions: This is the first study in Hamadan on genotyping of B. hominis, which may trigger other epidemiologic and zoonotic studies on different subtypes and hence control clinical manifestations of infection.

show abstract

Section: Discussionsupporting

confidence: 82%

A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

Sardarian

Hajilooi

Maghsood

et al. 2012

Jundishapur J Microbiol

View full text Add to dashboard Cite

show abstract

“…In many studies, pathogenic potential of subtype 3 has been reported (16,31,(33)(34)(35). Also, some investigations have demonstrated that other subtypes such as subtype 1 were associated with symptoms (20,31,34,36).…”

Section: Discussionmentioning

confidence: 99%

A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

Sardarian

Hajilooi

Maghsood

et al. 2012

Jundishapur J Microbiol

View full text Add to dashboard Cite

Blastocystis hominis is a common protozoan parasite in the human and many other animals intestine. the parasite pathogenicity is still controversial. A few studies have been conducted on prevalence of B.hominis subtypes in Iran. The results of present study improve our knowledge on B. hominis subtypes in Iran and therefore we can do more investigations on pathogenicity potential of these subtypes in the future. Background:Blastocystis is a common protozoan parasite in mammals, birds, amphibians, reptiles, fish, arthropods, and annelids. This parasite has some subtypes, which pathogenicity status of them still remained controversial. Some of Blastocystis subtypes are potentially pathogenic to human. Objectives: This study has identified the B. hominis subtypes and their prevalence rates in Hamadan. Patients and Methods: During two months of summer 2011, a total number of 250 human fecal samples referred for parasitology examination to Beasat Hospital and a few clinical laboratories of Hamadan city were collected. The samples were examined by direct method and formalin-ether. 41 samples exhibited positive results for B. hominis thereby were cultured in Locke-egg medium. After the growth and in order to genotype identification, B. hominis isolates were amplified by PCR, using seven pairs of sequencestagged site primers. . Results: In this study, three subtypes of B. hominis consisted of one [SB83], two [SB340] and three [SB227] were identified. The most dominant genotype was SB83 with 56.1% frequency. The prevalence rate of genotype SB227 and SB340 were 22% and 7.3%, respectively. Coexistence of genotypes SB83 and SB227 was detected in 14.6% of positive cases. Conclusions: This is the first study in Hamadan on genotyping of B. hominis, which may trigger other epidemiologic and zoonotic studies on different subtypes and hence control clinical manifestations of infection.

show abstract

“…Although these are routinely cited as a common human intestinal parasite or pathogen and have been suggested as an aetiological agent in many GI illness, including diarrhoea, inflammatory bowel disease and irritable bowel syndrome (Giacometti et al, 1999;Yakoob et al, 2004;Stark et al, 2007), it is believed that the potential pathogenicity of Blastocystis is subtype-dependent (Kaneda et al, 2001;Tan et al, 2006). Genetic and polymorphic variations of Blastocystis are well reported in the literature and classification schemes based on 18S rRNA genes and elongation factor genes have been used to infer phylogenetic relationships and discrete lineages (Ho et al, 2000;Abe, 2004;Yoshikawa et al, 2004), and the phylogenetic tree constructed from Blastocystis sequences obtained from different individuals analysed in this study falls into distinct phylogenetic clades or subtypes that have been previously defined (Arisue et al, 2003;Stensvold et al, 2007a).…”

Section: Resultsmentioning

confidence: 99%

Micro-eukaryotic diversity of the human distal gut microbiota: qualitative assessment using culture-dependent and -independent analysis of faeces

2008

View full text Add to dashboard Cite

Molecular ecological surveys of the human gut microbiota to date have focused on the prokaryotic fraction of the community and have revealed a remarkable degree of bacterial diversity and functionality. However, there is a dearth of information on the eukaryotic composition of the microbiota, and no culture-independent sequence-based surveys of human faeces are available. Culture-independent analyses based on DNA extraction and polymerase chain reaction targeting both the total eukaryotic 18S rRNA genes and fungal internal transcribed regions (ITS), together with culture-dependent analyses of fungi, were performed on a group of healthy volunteers. Temporal analysis was also included wherever possible. Collectively, the data presented in this study indicate that eukaryotic diversity of the human gut is low, largely temporally stable and predominated by different subtypes of Blastocystis. Specific analyses of the fungal populations indicate that a disparity exists between the cultivable fraction, which is dominated by Candida sp, and cultureindependent analysis, where sequences identical to members of the genera Gloeotinia/Paecilomyces and Galactomyces were most frequently retrieved from both fungal ITS profiles and subsequent clone libraries. Collectively, these results highlight the presence of unprecedented intestinal eukaryotic inhabitants whose functional roles are as yet unknown in healthy individuals. Furthermore, differences between results obtained from traditionally employed culture-based methods and those obtained from culture-independent techniques highlight similar anomalies to that encountered when first analysing the bacterial diversity of the human faecal microbiota using culture-independent surveys.

show abstract

Ribodemes of Blastocystis hominis isolated in Japan.

Cited by 92 publications

References 22 publications

A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

Micro-eukaryotic diversity of the human distal gut microbiota: qualitative assessment using culture-dependent and -independent analysis of faeces

Contact Info

Product

Resources

About