Riboflavin and ultraviolet light for pathogen reduction of murine cytomegalovirus in blood products

Abstract

These results suggest that using riboflavin and UV light treatment may be able to reduce the occurrence of transmission of human CMV from infectious PLTs and plasma units.

Inactivation of viruses in platelet and plasma products using a riboflavin‐and‐UV–based photochemical treatment

“…One potential way to provide an additional layer of safety to help prevent transfusion transmission of viruses is through the use of a broad‐spectrum pathogen reduction process. The Mirasol Pathogen Reduction Technology (PRT) System for Platelets and Plasma, a system that utilizes riboflavin as a photosensitizer in combination with a UV light illumination device (Terumo BCT, Lakewood, CO), has been developed to reduce the infectivity of a broad range of blood‐borne pathogens . The purpose of this study was to expand on the existing published viral reduction data and establish the effectiveness of this PRT system against a broad range of viral families.…”

“…The Mirasol Pathogen Reduction Technology (PRT) System for Platelets and Plasma, a system that utilizes riboflavin as a photosensitizer in combination with a UV light illumination device (Terumo BCT, Lakewood, CO), has been developed to reduce the infectivity of a broad range of blood-borne pathogens. [17][18][19][20][21][22][23] The purpose of this study was to expand on the existing published viral reduction data and establish the effectiveness of this PRT system against a broad range of viral families. In this study the reduction of encephalomyocarditis virus (EMC), hepatitis A virus (HAV), HCV, influenza A (FLUAV), La Crosse virus (LACV), pseudorabies virus (PRV), sindbis virus (SINV), and vesicular stomatitis virus (VSV) was evaluated in either plasma or PLT units using in vitro cell culture assay systems (see Table 1 for virus characteristics).…”

Interpretation of pathogen load in relationship to infectivity and pathogen reduction efficacy

“…A third example of lack of correlation between in vitro effects of NAT and infection transmission was reported by Keil et al who showed only a moderate reduction of cytomegalovirus (CMV) in vitro from riboflavin and ultraviolet light, (10 2.1 ) but complete prevention of CMV infection transmission from10 6 plaque forming units in an animal model. The diversity of data and way of expressing results in these examples illustrates the complex nature of this issue and the difficulty drawing simple conclusions regarding the likelihood of infection transmission.…”

Current strategies and future directions for the prevention of transfusion-transmitted cytomegalovirus

“…84 Both technologies have been shown to prevent the transmission of murine CMV by platelet transfusion in mouse models. 85,86 In addition to the benefit of preventing TT-CMV, pathogen inactivation also prevents the transmission of bacteria and many other viruses. It also decreases the risk of transfusion-associated graft versus host disease through the inactivation of donor leukocytes, possibly obviating the need for irradiation of blood products in at-risk patients.…”