Riboflavin–citrate conjugate multicore SPIONs with enhanced magnetic responses and cellular uptake in breast cancer cells

Abstract

Breast cancer accounts up to 10% of the newly diagnosed cancer cases worldwide, making it the most common cancer found in women. The use of superparamagnetic iron oxide nanoparticles (SPIONs)...

Proteomic Analysis Reveals Distinct Protein Corona Compositions of Citrate- and Riboflavin-Coated SPIONs

“…At prolonged incubation, both kinds of corona formation showed a similar uptake pattern where the highest activity was observed from Rf-SPIONs, followed by C- and B-SPIONs, respectively. The results highlighted the properties of the Rf ligand in promoting the cellular uptake of SPIONs in the breast cancer cells as reported earlier . With no reduction of uptake levels in both cellular uptake processes, our hypotheses are that the corona proteins do not affect the activity of the Rf ligand in enhancing the cellular internalization or that there are key components in the corona influencing the uptake mechanism of the NPs.…”

“…In the past decades, superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively studied and utilized as contrasting agents in magnetic resonance imaging (MRI) and for treatment in magnetic hyperthermia (MH) due to their excellent biocompatibility and magnetic properties. To improve their stability in biological fluids and increase their specificity to target sites, several surface coatings have been introduced. , Different surface coatings can lead to different preferences of protein binding on the surface of nanoparticles (NPs) called “protein corona”, which ultimately determines its behavior in vitro and in vivo . The most evident outcome is in the interaction of NPs with macrophages which directly affect their circulation time. , Furthermore, formation of protein coronas can alter the toxicity of the NPs toward mammalian cells in both positive , and negative ways. , Therefore, the study of protein corona compositions, even though not sufficient for full prediction, could provide us a brief context of how the NPs would interact with their biological environments, which is important for their safe design and efficient biomedical applications.…”

“…Citric acid is one of the most commonly used anionic surface stabilizers for SPIONs . In addition, riboflavin (Rf) is also a promising ligand that has been increasingly used on several types of particles such as PLGA, dendrimer, or SPIONs due to its property of enhancing cellular uptake in several estrogen-related cancer cells. , It has been shown recently by our group that multicore SPIONs with both surface coatings have excellent properties as MRI contrast agents and MH agents . The SPIONs, having a clustered morphology, exhibit exceptionally high T 2 values, which can be considered as one of the highest T 2 responses among the SPIONs synthesized from co-precipitation methods.…”

Magneto-optical hyperthermia agents based on probiotic bacteria loaded with magnetic and gold nanoparticles

“…2,3 In particular, gold nanoparticles (AuNPs) and superparamagnetic maghemite/magne-tite nanoparticles (MNPs) are being studied in the frame of hyperthermia treatments applied to tumors. 4,5 Hyperthermia involves killing cells by heating them to temperatures higher than those which normally occur in the body, typically around 40-50 °C. 6 Heating small areas of the tumor causes cancer cell death by denaturing their proteins and damaging their membranes.…”

“…2,3 In particular, gold nanoparticles (AuNPs) and superparamagnetic maghemite/magnetite nanoparticles (MNPs) are being studied in the frame of hyperthermia treatments applied to tumors. 4,5…”