1992
DOI: 10.1042/bst0200764
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Ribonuclease H-mediated inhibition of translation and reverse transcription by antisense oligodeoxynucleotides

Abstract: Biochemical Society Transactionslikely efficacy of antisense oligodeoxynucleotides as chemotherapeutic agents. Two major problems remain. The first of these, as mentioned earlier, is the stability of the ODNs in cell systems. Our present experiments suggest that the half life of these is between 1 and 3 h, which is unlikely to be sufficient for a clinical preparation. The second concerns the efficiency of uptake into the cells which, while we have not yet quantified it, we believe is unlikely to be greater tha… Show more

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Cited by 8 publications
(1 citation statement)
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“…Our AS-ODN was designed to hybridize to its specific mRNA target, which has been shown to create a conformational obstacle for protein translation (KretschmerKazemi Far and Sczakiel, 2003). Previous studies have demonstrated that AS-ODN could enter cells and stop protein translation by either blocking the translocation of ribosomes (Boiziau et al, 1992) or destroying the target mRNA through RNase H-mediated degradation (Sommer et al, 1998). Intracerebroventricular (i.c.v) infusion of AS-ODN caused a decrease in nNOS mRNA but not NOS enzymatic activity in rat hippocampus (Kolesnikov et al, 1997).…”
Section: Discussionmentioning
confidence: 99%
“…Our AS-ODN was designed to hybridize to its specific mRNA target, which has been shown to create a conformational obstacle for protein translation (KretschmerKazemi Far and Sczakiel, 2003). Previous studies have demonstrated that AS-ODN could enter cells and stop protein translation by either blocking the translocation of ribosomes (Boiziau et al, 1992) or destroying the target mRNA through RNase H-mediated degradation (Sommer et al, 1998). Intracerebroventricular (i.c.v) infusion of AS-ODN caused a decrease in nNOS mRNA but not NOS enzymatic activity in rat hippocampus (Kolesnikov et al, 1997).…”
Section: Discussionmentioning
confidence: 99%