Ribonucleases in Leaves and Roots of Triticum durum and x Haynaldoticum sardoum Submitted to Low non Freezing Temperatures

SpanÒ, Larmelina; Urilli, Isa; Sbrana, Valeriano; Meletti, Paolo

doi:10.1016/s0176-1617(99)80149-7

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…RNase activity: Electrophoresis was performed according to Blank et al (1982) and Blank and McKeon (1991), with minor modifications (Spanò et al 1999). RNase activity analyses were carried out using 12.5 % SDS-PAGE gels containing 2.4 mg cm -3 Torula yeast RNA.…”

Section: Rnases Extraction and Enzymatic Assaymentioning

confidence: 99%

Dormancy and germination in wheat embryos: ribonucleases and hormonal control

Spanò¹,

Buselli²,

Grilli³

2008

Biologia plant.

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Acidic and neutral ribonucleases (RNases) were studied in embryos of Triticum durum cv. Cappelli and the effects of abscisic acid (ABA) and gibberellic acid (GA 3 ) were analysed. RNases activities increased during germination and were comparable in dormant and non-dormant embryos imbibed for 24 h. ABA generally inhibited ribonucleolytic activities, while GA 3 only affected dormant embryos. To assess whether changes in RNase activities during germination or following hormonal treatment required new transcriptional or translational action, cycloheximide or cordycepin were used. The action of inhibitors of acidic RNase activity was found only in non-dormant-embryos. Findings obtained in the present work concur with a change of the ribonucleolytic pattern in the shift from dormant to non dormant metabolism.

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Section: Rnases Extraction and Enzymatic Assaymentioning

confidence: 99%

Dormancy and germination in wheat embryos: ribonucleases and hormonal control

Spanò¹,

Buselli²,

Grilli³

2008

Biologia plant.

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“…Electrophoresis was performed according to Blank and McKeon (1991), and Blank et al (1982), with minor modifications (Spanò et al, 1999).…”

Section: Rnase and Nuclease Activity Gelmentioning

confidence: 99%

“…In the present study, RNases and nucleases were examined using SDS-PAGE and activity staining (Spanò et al, 1999), to verify whether they are subject to regulation during ageing. Both embryos and endosperms were used, as it is known from the literature that the two seed compartments age independently (Floris, 1970).…”

Section: Introductionmentioning

confidence: 99%

RNases and nucleases in embryos and endosperms from naturally aged wheat seeds stored in different conditions

Spanò

Buselli

Castiglione

et al. 2007

Journal of Plant Physiology

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Ribonuclease activity in roots of soybean seedlings subjected to chilling stress and recovery

Stępiński

2002

Acta Physiol Plant

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Many developmental and physiological changes, including alterations of enzyme activities, occur in plants under low temperature stress. In this study the total ribonuclease activity was determined in crude extracts from root tips of soybean seedlings germinated at 25 °C, subjected to chilling conditions (10 °C) and recovered at optimal temperature (25 °C). Measurements of RNase activity were performed every 24 hours starting from the third to the t 0-th day of growth. We found that chilling caused a considerable increase in ribonuclease activity (in comparison with the control), with an activity peak on the fourth day of the cold treatment. The enzyme activity in root extracts of the plants recovered after cold stress decreased along with the time of recovery.No differences were found in approximate molecular weight (35 kDa) and pH optimum (6.0) for fibonucleases extracted from control and chilled soybean roots.

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Ribonucleases in Leaves and Roots of Triticum durum and x Haynaldoticum sardoum Submitted to Low non Freezing Temperatures

Cited by 5 publications

References 11 publications

Dormancy and germination in wheat embryos: ribonucleases and hormonal control

Dormancy and germination in wheat embryos: ribonucleases and hormonal control

RNases and nucleases in embryos and endosperms from naturally aged wheat seeds stored in different conditions

Ribonuclease activity in roots of soybean seedlings subjected to chilling stress and recovery

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