Ribonucleic acid and protein synthesis in ovary

Civen, Morton; Brown, Charlesta B.; Hilliard, Jessamine

doi:10.1016/0005-2787(66)90260-7

Cited by 13 publications

(2 citation statements)

References 6 publications

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“…Histochemical studies have suggested that gonadotrophins stimulate the general synthesis of nucleic acids (Jacoby 1962), and an increase in total RNA extracted by the method described by Schneider (1957) and determined by the orcinol assay (Drury 1948) has been observed in the prepubertal rat ovary after treatment with gonadotrophins (van Dyke and Katzman 1968). An increase in incorporation of labelled uridine into RNA of rabbit ovarian slices in uitro after pretreatment in U~D O with gonadotrophins has also been reported (Civen, Brown and Hilliard 1966). I n the last mentioned study, the incorporation of uridine was determined in ribonucleotides after treatment of the labelled RNA with pancreatic ribonuclease.…”

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Characterization of Ovarian RNA

Ahrén

Hamberger

Jarlstedt

et al. 1970

Acta Physiologica Scandinavica

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Ovaries were obtained from 24–25 day old Sprague‐Dawley rats. Groups of 20 ovaries were collected corresponding to a wet weight of 100–120 mg, and incubated for 20, 30, 60 and 240 min at 37° C in Krebs bicarbonate buffer containing 5.5 mM glucose and uridine‐3H. Following incubation, ovarian RNA was extracted with a modified phenol extraction method described in detail. Fractionation was made by composite agarose‐polyacrylamide gel electrophoresis which gave good separation and high resolution of the various RN.4 fractions. Ovarian RNA separated in four well‐defined fractions classified as 28S, 18S, 5s and 4s. This classification was based upon comparisons with simultaneously run liver RNA. In addition to the four main fractions. a weak band just before 28S and two smaller bands between 28S and 18S were consistently observed. Labelling of ovarian RNA with uridine‐3H showed a polydisperse pattern after the shorter incubation periods with most of the radioactivity concentrated to RNA fractions of molecular weights higher than 28S, but also clear labelling of the 5S—4S region. After 60 min, radioactivity peaks were also associated with the 28S and 18s fractions and with fractions between those, while after 240 min incubation, the radioactivity was concentrated to the four main fractions. The incorporation of radioactivity was found to be markedly decreased in presence of actinomycin D.

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confidence: 67%

Characterization of Ovarian RNA

Ahrén

Hamberger

Jarlstedt

et al. 1970

Acta Physiologica Scandinavica

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“…Samples were then taken from spleen, brain, testicles, ovaries, liver, kidney, leg mucles, and pectoral muscles. The proteins were isolated and weighed and their radioactivity was counted in a window gas flow counter according to the technique previously described by Civen et al (1966). The total nitrogen content of the samples was determined by the Kjeldahl method.…”

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Influence of Light on the Metabolism of 14 C Labelled Valine in Male and Female Broilers

1972

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The experiment involved using 2,750 male and female broilers to determine the effect of light on the metabolism of intravenously injected labelled valine. Control birds were subjected to 12.5 hours of natural daylight daily from the day of hatching to 70 days of age. Treated birds were subjected to continuous light from the day of hatching until 70 days of age (12.5 hours of natural daylight plus 11.5 hours of artificial light). Body weight, feed intake, feed conversion, and mortality were not affected by the treatment; however, metabolic activity was altered. The metabolic activity was investigated in 24 birds at 71 days of age. In general, respiratory M C0 2 production increased in the treated males by 29.8%. This difference was not significant. Respiratory 14 COj production decreased in the treated females by 35.4% (P < 0.01) as when compared with the control group.

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