Ribonucleic acid stimulation of mammalian liver nuclear-envelope nucleoside triphosphatase. A possible enzymic marker for the nuclear envelope

Agutter, Paul S.; Harris, J. Robin; Stevenson, I. H.

doi:10.1042/bj1620671

Cited by 64 publications

(36 citation statements)

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“…3(b) is more rapid than step IV (i.e. if RNA binding to structure S stimulates dephosphorylation), this scheme accounts for the stimulation of NTPase activity by RNA (Agutter et al, 1977). The effect of AMP + PP, on RNA release ( Fig.…”

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confidence: 99%

Influence of nucleotides, cations and nucleoside triphosphatase inhibitors on the release of ribonucleic acid from isolated rat liver nuclei

Agutter¹

1980

Biochemical Journal

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The reasons underlying reported discrepancies in the effects of ATP, ADP, adenosine 5'-[Ify-methyleneltriphosphate, AMP + PP1, p-chloromercuribenzoate and F-on RNA efflux from isolated rat liver nuclei and on nuclear-envelope nucleoside triphosphatase activity were investigated. The stimulatory effect of ADP was attributed to myokinase activity associated with the nuclei; this activity was eluted on repeated washing with nuclear incubation medium. In the absence of Ca2+ and Mn2+, ATP, adenosine 5'-[Iymethyleneltriphosphate and AMP+ PP1 were found to promote release of both DNA and RNA. In the presence of 0.5 mM-Ca2+ and 0.3 mM-Mn2 , only ATP promoted RNA efflux to a significant extent. In the absence of spermidine, Ca2+ and Mn2+, nuclei released large quantities of DNA and RNA into the medium; this effect was promoted by p-chloromercuribenzoate. In the presence of the three cations, however, pchloromercuribenzoate inhibited RNA efflux. F-caused a slight leakage of DNA from nuclei. The results are discussed in terms of models for the effects of ATP and analogues on RNA efflux and nuclear stability.The stimulation of RNA release from isolated nuclei by ATP and related compounds has been investigated in several laboratories and the effect has been interpreted in at least four different ways.(1) The ATP chelates bivalent metal ions in the medium, promoting release of heterogeneous nuclear RNA-protein complexes from the chromatin (Ishi-

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Influence of nucleotides, cations and nucleoside triphosphatase inhibitors on the release of ribonucleic acid from isolated rat liver nuclei

Agutter¹

1980

Biochemical Journal

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“…Clawson et al (1980) observed stimulation of nucleoside triphosphatase activity in calf and rat liver nuclear envelope by yeast RNA, and they suggested that the stimulatory effect of RNA may arise through a substrate effect if the nuclear-envelope enzyme requires the presence of an RNA chain. Lam & Casper (1979) and Agutter et al (1976and Agutter et al ( , 1977and Agutter et al ( , 1979 showed a preferential phosphorylation and dephosphorylation of a particular nuclear-membrane protein of 68 000 mol.wt. during RNA transport in vitro from rat liver nuclei.…”

Section: Discussionmentioning

confidence: 99%

“…A partial enrichment of the factors was obtained by (NH4)2S04 fractionation of the cytosol, and the 40-80%-satd.-(NH4)2SO4 fraction showed the maximum activity (results not shown). Agutter et al (1977) RNA transport of membrane-bound nucleoside triphosphatase, which may be stimulated by exogenous RNA. A similar involvement of alkaline phosphatase was reported by Cutler et al (1974).…”

Section: Enzyme Activitiesmentioning

confidence: 99%

Effect of cytosol on transport of RNA in vitro during cardiac hypertrophy

Meenakshi¹,

Thirunavukkarasu²,

Rajamanickam³

1983

Biochemical Journal

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An enhanced RNA-transport activity was observed in vitro from nuclei obtained from animals with cardiac hypertrophy as compared with that of sham-operated controls. The 100lOOg supernatants obtained from hypertrophic hearts stimulated the RNA transport from nuclei of sham-operated controls, and this stimulation was maximum with 40% supernatant. Ca2+-and nucleic acid-dependent ATPase and alkaline phosphatase activities, which may be involved in an energy-dependent transport, were high in nuclei from hypertrophic hearts, and the nuclei of sham-operated animals showed higher activities of these enzymes after incubation with supernatant from hypertrophic hearts, which stimulates the sham-operated animals.Transcriptional and post-transcriptional regulation and flow of genetic information from nucleus to cytoplasm in eukaryotic systems have been widely studied (Drews et al., 1968;Stevenin et al., 1969;Levitan & Webb, 1970;Lichtenstein & Shapot, 1976). By cell fusion and nuclear-transplantation experiments it was found that cytoplasmic factors regulate the expression of genes through feedback controls (Gurdon & Uehlinger, 1966;Harris, 1970). These feedback-control mechanisms operate not only at the transcriptional level but also at the post-transcriptional level. The availability of a cell-free system that can support nearly normal RNA processing and transport has made it possible to study the transport of RNA in vitro. In the present paper we report the occurrence of induced transport of RNA in vitro from the nuclei obtained from hypertrophic hearts compared with that from normal hearts. Schumm & Webb (1972 and Schumm et al. (1973) buffer for 18h at 40C with two changes, and 4.5mg of non-diffusable material was used along with 1 ml of incubation medium. After incubation the nuclei were pelleted by centrifugation at 100000 g (rav.5.35 cm) for 1 h, and RNA was extracted from the supernatant by the method of Penman (1966). The percentage of RNA was calculated from the radioactivity (c.p.m.) in the RNA released into the supernatant per mg of cytosolic protein. Enzyme activitiesATPase activity was assayed by the method of Tzagoloff (1970)

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“…Furthermore, Agutter et al (1977) found that ATPase activity depends on RNA, suggesting the presence of the enzyme in the nucleocytoplasmic transport of RNPs.…”

Section: Mgmentioning

confidence: 99%

Mg 2+ -dependent ATPase activity in triatomine salivary glands (Heteroptera, Triatominae)

Anhê

Azeredo-Oliveira

2017

Iheringia, Sér. Zool.

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ABSTRACT. Mg 2+ -ATPase activity was detected in the three salivary glands of adult triatomines, males and females, of Triatoma infestans (Klug, 1834) and Panstrongylus megistus (Burmeister, 1835) (Heteroptera, Triatominae). A predominance of binucleated cells in D1 and D2 and mononucleated in D3 was observed, with bulky and polyploidy nuclei. ATPase activity was detected in the nuclei, possibly in euchromatin and nucleolus, where this enzyme probably acts in the transcription process. ATPase reaction was also evidenced in the nuclear membrane, which is probably associated with nuclearcytoplasmatic transport. These characteristics indicate a high metabolism and protein synthesis, which must be essential to saliva production as well as in maintaining the hematophagy of triatomines.KEYWORDS. Euchromatin, gland, nuclear membrane, nucleolus, phosphatase. RESUMO. Atividade da enzima ATPase dependente de MG 2+ em glândulas salivares de triatomíneos (Heteroptera, Triatominae). A atividade da enzima ATPase dependente de Mg2+ foi detectada nas três glândulas salivares de triatomíneos adultos, machos e fêmeas, de Triatoma infestans (Klug, 1834) e Panstrongylus megistus (Burmeister, 1835) (Heteroptera, Triatominae). Observou-se um predomínio de células binucleadas em D1 e D2, e mononucleadas em D3, com núcleos volumosos e poliplóides. Atividade da ATPase foi detectada no núcleo, provavelmente na eucromatina e no nucléolo, onde esta enzima atuaria no processo de transcrição. A atividade também foi evidenciada na membrana nuclear e, possivelmente, associada ao transporte núcleo-citoplasmático. Essas características indicam alto metabolismo e elevada síntese proteica, essenciais para a produção de saliva e manutenção da hematofagia de triatomíneos.

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Ribonucleic acid stimulation of mammalian liver nuclear-envelope nucleoside triphosphatase. A possible enzymic marker for the nuclear envelope

Cited by 64 publications

References 36 publications

Influence of nucleotides, cations and nucleoside triphosphatase inhibitors on the release of ribonucleic acid from isolated rat liver nuclei

Influence of nucleotides, cations and nucleoside triphosphatase inhibitors on the release of ribonucleic acid from isolated rat liver nuclei

Effect of cytosol on transport of RNA in vitro during cardiac hypertrophy

Mg 2+ -dependent ATPase activity in triatomine salivary glands (Heteroptera, Triatominae)

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